Recombinant Human Uracil phosphoribosyltransferase homolog/UPRT is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Asp309) of Human UPRT fused with a 6His tag at the N-terminus.
Reinheit
> 95 % as determined by reducing SDS-PAGE.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl pH 8.0.
Handhabung
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Lagerung
-80 °C
Informationen zur Lagerung
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Gm774 Protein, FUR1 Protein, UPP Protein, uracil phosphoribosyltransferase Protein, uracil phosphoribosyltransferase homolog Protein, Uprt Protein, UPRT Protein
Sub Type
Fusionprotein
Hintergrund
UPRT is a cytoplasmic enzyme which belongs to the UPRTase family. UPRT is highly expressed in leukocytes, liver, spleen and thymus, with lower expression in brain, lung and skeletal muscle. UPRTcatalyzes the conversion of uracil and 5-phosphoribosyl-1-R-diphosphate to uridine monophosphate (UMP). This reaction is an important part of nucleotide metabolism, specifically the pyrimidine salvage pathway. UPRT is a potential target for rational design of drugs to treat parasitic infections and cancer Alternative Names: Uracil phosphoribosyltransferase homolog, UPP, FUR1, UPRT