BDH1 Protein (AA 1-343) (His tag)

Produktnummer ABIN1691949

Produktdetails

Target: BDH1 (3-hydroxybutyrate Dehydrogenase, Type 1 (BDH1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-343

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses BDH1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human D-β-Hydroxybutyrate Dehydrogenase Mitochondrial/BDH1 (N-6His)

Sequenz: MGSSHHHHHH SSGLVPRGSH MLATRLSRPL SRLPGKTLSA CDRENGARRP LLLGSTSFIP IGRRTYASAA EPVGSKAVLV TGCDSGFGFA LAKHLHSKGF LVFAGCLMKD KGHDGVKELD SLNSDRLRTV QLNVCSSEEV EKVVEIVRSS LKDPEKGMWG LVNNAGISTF GEVEFTSLET YKQVAEVNLW GTVRMTKSFL PLIRRAKGRV VNISSMLGRM ANPARSPYCI TKFGVEAFSD CLRYEMYPLG VKVSVVEPGN FIAATSLYSP ESIQAIAKKM WEELPEVVRK DYGKKYFDEK IAKMETYCSS GSTDTSPVID AVTHALTATT PYTRYHPMDY YWWLRMQIMT HLPGAISDMI YIR

Produktmerkmale: Recombinant Human D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial/BDH1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Arg343) of Human BDH1 fused with a 6His tag at the N-terminus.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM Tris, 1 mM DTT, pH 7.5.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: -80 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: BDH1 (3-hydroxybutyrate Dehydrogenase, Type 1 (BDH1))

Andere Bezeichnung: D-beta-Hydroxybutyrate Dehydrogenase Mitochondrial/BDH1 (BDH1 Produkte)

Sub Type: Fusionprotein

Hintergrund: D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial (BDH) is a member of the short-chain dehydrogenases/reductases (SDR) family. BDH is localized in the mitochondrion matrix. BDH forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific necessity for phosphatidylcholine for optimal enzymatic activity. BDH catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the 2 main ketone bodies formed during fatty acid catabolism.
Alternative Names: D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial, BDH, 3-Hydroxybutyrate Dehydrogenase, BDH

Molekulargewicht: 40.3 kDa

UniProt: Q02338

Pathways: Response to Growth Hormone Stimulus