Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G) (Active) protein (His tag)

Details zu Produkt Nr. ABIN1526013, Anbieter: Anmelden zum Anzeigen
Proteinname
  • AI385652
  • Fin13
  • PP2CG
  • PP2CGAMMA
  • PPP2CG
  • protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
  • protein phosphatase, Mg2+/Mn2+ dependent 1G
  • protein phosphatase, Mg2+/Mn2+ dependent, 1G
  • Ppm1g
  • PPM1G
Spezies
Human
19
1
Quelle
Escherichia coli (E. coli)
11
2
2
2
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses PPM1G Protein ist gelabelt mit His tag.
Applikation
SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Sequenz MGSSHHHHHH SSGLVPRGSH MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELD SETAMFSVYD GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGR PTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEA GPEDSTRETP SQENGPTAKA YTGFSSNSER GTEAGQVGEP GIPTGEAGPS CSSASDKLPR VAKSKFFEDS EDESDEAEEE EEDSEECSEE EDGYSSEEAE NEEDEDDTEE AEEDDEEEEE EMMVPGMEGK EEPGSDSGTT AVVALIRGKQ LIVANAGDSR CVVSEAGKAL DMSYDHKPED EVELARIKNA GGKVTMDGRV NGGLNLSRAI GDHFYKRNKN LPPEEQMISA LPDIKVLTLT DDHEFMVIAC DGIWNVMSSQ EVVDFIQSKI SQRDENGELR LLSSIVEELL DQCLAPDTSG DGTGCDNMTC IIICFKPRNT AELQPESGKR KLEEVLSTEG AEENGNSDKK KKAKRD
Produktmerkmale Molecule Information: 61.4 kDa (566 aa, 1-546 aa + NT His-Tag)
Reinheit ≥85 % by SDS-PAGE
Biological Activity Comment Specific activity: >5,000 units/mg.
Unit Definition One unit will hydrolyze 1 nmole of p-nitrophenylphosphatate (pNPP) per minute at 37°C, pH 7.5 using 10 mM of substrate.
Proteinname Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G)
Hintergrund PPM1G is part of the PP2C family of Ser/Thr protein phosphatases which are known to be negative regulators of cell stress response pathways. PPM1G is accountable for the dephosphorylation of Pre-mRNA splicing factors, an important factor for the formation of functional spliceosome. PPM1G regulates cell cycle progression. PPM1G mediates histone dephosphorylation/exchange in response to DNA damage or checkpoint recovery in higher eukaryotes. The degradation of p21/WAF1 induced by PPM1G is mediated in a proteasome-dependent manner. Protein phosphatase 1G regulates assembly and function of the beta-catenin degradation complex.

Alternative Names: Protein phosphatase 1G, PP2CG, PPP2CG, MGC1675, MGC2870, PP2CGAMMA.
Molekulargewicht 61.4 kDa
Gen-ID 5496
UniProt O15355
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer 1 mg/mL solution in 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 1 mM DTT, 0.1 M NaCl, and 0.1 mM PMSF.
Konservierungs-mittel Dithiothreitol (DTT)
Vorsichtsmaßnahmen This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Centrifuge the vial prior to opening.
Lagerung -80 °C
Haltbarkeit 12 months
Bilder des Herstellers
SDS-PAGE (SDS) image for Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G) (Active) protein (His tag) (ABIN1526013) Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G) (Active) protein (His tag)
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