MDM2 Protein (AA 1-491) (GST tag)
(1 reference)-
- Target Alle MDM2 Proteine anzeigen
- MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-491
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Spezies
- Human
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Quelle
- Wheat germ
- Aufreinigungstag / Konjugat
- Dieses MDM2 Protein ist gelabelt mit GST tag.
- Applikation
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Verwendungszweck
- MDM2 (Human) Recombinant Protein (P01)
- Sequenz
- MCNTNMSVPT DGAVTTSQIP ASEQETLVRP KPLLLKLLKS VGAQKDTYTM KEVLFYLGQY IMTKRLYDEK QQHIVYCSND LLGDLFGVPS FSVKEHRKIY TMIYRNLVVV NQQESSDSGT SVSENRCHLE GGSDQKDLVQ ELQEEKPSSS HLVSRPSTSS RRRAISETEE NSDELSGERQ RKRHKSDSIS LSFDESLALC VIREICCERS SSSESTGTPS NPDLDAGVSE HSGDWLDQDS VSDQFSVEFE VESLDSEDYS LSEEGQELSD EDDEVYQVTV YQAGESDTDS FEEDPEISLA DYWKCTSCNE MNPPLPSHCN RCWALRENWL PEDKGKDKGE ISEKAKLENS TQAEEGFDVP DCKKTIVNDS RESCVEENDD KITQASQSQE SEDYSQPSTS SSIIYSSQED VKEFEREETQ DKEESVESSL PLNAIEPCVI CQGRPKNGCI VHGKTGHLMA CFTCAKKLKK RNKPCPVCRQ PIQMIVLTYF P
- Produktmerkmale
- Human MDM2 full-length ORF ( ENSP00000258149, 1 a.a. - 491 a.a.) recombinant protein with GST-tag at N-terminal.
- Aufreinigung
- in vitro wheat germ expression system
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Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 1-118) protein (His tag)
MDM2 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 1-300) protein (His tag)MDM2 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 101-200) protein (GST tag)MDM2 Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 1-489) protein (Strep Tag)Crystallography grade MDM2 Spezies: Maus Wirt: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 1-491) protein (Strep Tag)Crystallography grade MDM2 Spezies: Human Wirt: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 1-489), (full length) protein (His tag)MDM2 Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 90 % SDS
Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 1-218) (Active) protein (His tag)MDM2 Spezies: Human Wirt: Escherichia coli (E. coli) > 95 % AcA, CC Active
Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 1-489), (full length) protein (His tag)MDM2 Spezies: Maus Wirt: Hefe Recombinant > 90 % SDS
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handhabung
- Aliquot to avoid repeated freezing and thawing.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Best use within three months from the date of receipt of this protein.
-
-
: "LC-MS/MS-based targeted proteomics quantitatively detects the interaction between p53 and MDM2 in breast cancer." in: Journal of proteomics, Vol. 152, pp. 172-180, (2016) (PubMed).
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: "LC-MS/MS-based targeted proteomics quantitatively detects the interaction between p53 and MDM2 in breast cancer." in: Journal of proteomics, Vol. 152, pp. 172-180, (2016) (PubMed).
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- Target
- MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
- Andere Bezeichnung
- MDM2 (MDM2 Produkte)
- Synonyme
- ACTFS Protein, HDMX Protein, hdm2 Protein, zgc:109834 Protein, 1700007J15Rik Protein, AA415488 Protein, Mdm-2 Protein, MDM2 proto-oncogene Protein, MDM2 proto-oncogene S homeolog Protein, Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) Protein, MDM2 oncogene, E3 ubiquitin protein ligase Protein, transformed mouse 3T3 cell double minute 2 Protein, MDM2 Protein, mdm2.S Protein, Mdm2 Protein, mdm2 Protein
- Hintergrund
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Full Gene Name: Mdm2 p53 binding protein homolog (mouse)
Synonyms: HDMX,MGC71221,hdm2 - Gen-ID
- 4193
- Pathways
- p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Autophagie, Ubiquitin Proteasome Pathway
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