HIF1A Protein (AA 1-735) (GST tag)

Produktnummer ABIN1306618

Produktdetails

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-735

Spezies: Human

Quelle: Wheat germ

Aufreinigungstag / Konjugat: Dieses HIF1A Protein ist gelabelt mit GST tag.

Applikation: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Verwendungszweck: HIF1A (Human) Recombinant Protein (P01)

Sequenz: MEGAGGANDK KKISSERRKE KSRDAARSRR SKESEVFYEL AHQLPLPHNV SSHLDKASVM RLTISYLRVR KLLDAGDLDI EDDMKAQMNC FYLKALDGFV MVLTDDGDMI YISDNVNKYM GLTQFELTGH SVFDFTHPCD HEEMREMLTH RNGLVKKGKE QNTQRSFFLR MKCTLTSRGR TMNIKSATWK VLHCTGHIHV YDTNSNQPQC GYKKPPMTCL VLICEPIPHP SNIEIPLDSK TFLSRHSLDM KFSYCDERIT ELMGYEPEEL LGRSIYEYYH ALDSDHLTKT HHDMFTKGQV TTGQYRMLAK RGGYVWVETQ ATVIYNTKNS QPQCIVCVNY VVSGIIQHDL IFSLQQTECV LKPVESSDMK MTQLFTKVES EDTSSLFDKL KKEPDALTLL APAAGDTIIS LDFGSNDTET DDQQLEEVPL YNDVMLPSPN EKLQNINLAM SPLPTAETPK PLRSSADPAL NQEVALKLEP NPESLELSFT MPQIQDQTPS PSDGSTRQSS PEPNSPSEYC FYVDSDMVNE FKLELVEKLF AEDTEAKNPF STQDTDLDLE MLAPYIPMDD DFQLRSFDQL SPLESSSASP ESASPQSTVT VFQQTQIQEP TANATTTTAT TDELKTVTKD RMEDIKILIA SPSPTHIHKE TTSATSSPYR DTQSRTASPN RAGKGVIEQT EKSHPRSPNV LSVALSQRTT VPEEELNPKI LALQNAQRKR KMEHDGSLFQ AVGII

Produktmerkmale: Human HIF1A full-length ORF ( NP_851397.1, 1 a.a. - 735 a.a.) recombinant protein with GST-tag at N-terminal.

Aufreinigung: in vitro wheat germ expression system

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handhabung: Aliquot to avoid repeated freezing and thawing.

Lagerung: -80 °C

Informationen zur Lagerung: Best use within three months from the date of receipt of this protein.

Referenzen

Vadivel, Alphonse, Etches, van Haaften, Collins, OReilly, Eaton, Thébaud: "Hypoxia-inducible factors promote alveolar development and regeneration." in: American journal of respiratory cell and molecular biology, Vol. 50, Issue 1, pp. 96-105, (2014) (PubMed).

Yang, Li, Yin, Huang, Sun, Li: "A new method to assay hypoxia-inducible factor-1 based on small molecule binding DNA." in: Analytica chimica acta, Vol. 838, pp. 31-6, (2014) (PubMed).

Yang, Wang, Humphries, Hogan, OShea, Moynagh: "The E3 ubiquitin ligase Pellino3 protects against obesity-induced inflammation and insulin resistance." in: Immunity, Vol. 41, Issue 6, pp. 973-87, (2014) (PubMed).

Antigendetails

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Andere Bezeichnung: HIF1A (HIF1A Produkte)

Synonyme: hif1a Protein, AA959795 Protein, HIF1alpha Protein, MOP1 Protein, bHLHe78 Protein, HIF-1A Protein, HIF-1alpha Protein, HIF1 Protein, HIF1-ALPHA Protein, PASD8 Protein, HIF1-alpha Protein, hif-1a Protein, hypoxia inducible factor 1 alpha Protein, hypoxia inducible factor 1, alpha subunit Protein, hypoxia inducible factor 1 alpha subunit Protein, HIF (Hypoxia Inducible Factor) homologa Protein, hif-1a Protein, Hif1a Protein, HIF1A Protein, hif1a Protein, hif-1 Protein

Hintergrund: Full Gene Name: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Synonyms: HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78

Gen-ID: 3091

NCBI Accession: NM_181054

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effekt