IL-13 Protein

Produktnummer ABIN1305069

Produktdetails

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func)

Marke: BD Pharmingen™

Spezifität: Activity Assay: Measured by proliferation using TF-1 indicator cells
Specific Activity Range: 0.6 - 5 x 10^7 Units /mg
ED50: 0.2 - 1.5 ng/ml
Observed dose response relationship: 50 fold
Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation.

Produktmerkmale: Recombinant mouse IL-13 is supplied as a frozen aqueous buffered solution containing 1.0 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant mouse IL-13 is ≥95% pure, as determined by SDS-PAGE, and an absorbance assay based on the Beers- Lambert law.

Aufreinigung: Recombinant mouse IL-13 is ≥95 % pure, as determined by SDS-PAGE, and an absorbance assay based on the Beers- Lambert law.

Reinheit: ≥ 95 %

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: The endotoxin level is ≤ 0.1 ng per μg of mouse IL-13, as measured in a chromogenic LAL assay.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

BD Pharmingen™ Recombinant Mouse IL-13

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: Recombinant mouse IL-13 is supplied as a frozen aqueous buffered solution containing 1.0 mg/mL biotechnology grade, low endotoxin bovine serum albumin, with no preservatives.

Konservierungsmittel: Azide free

Handhabung: Rapidly thaw and quick-spin product prior to use. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, prepare stock solutions diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine serum albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 5 µg/ml for long term storage.
Note: Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. Rapidly thaw and quick-spin product prior to use. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, prepare stock solutions diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine serum albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 5 μg/ml for long term storage. Note: Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

Referenzen

Doherty, Kastelein, Menon, Andrade, Coffman: "Modulation of murine macrophage function by IL-13." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 12, pp. 7151-60, (1994) (PubMed).

Zurawski, de Vries: "Interleukin 13, an interleukin 4-like cytokine that acts on monocytes and B cells, but not on T cells." in: Immunology today, Vol. 15, Issue 1, pp. 19-26, (1994) (PubMed).

Kitamura, Tange, Terasawa, Chiba, Kuwaki, Miyagawa, Piao, Miyazono, Urabe, Takaku: "Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin." in: Journal of cellular physiology, Vol. 140, Issue 2, pp. 323-34, (1989) (PubMed).

Antigendetails

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Andere Bezeichnung: IL-13 (IL13 Produkte)

Synonyme: IL13 Protein, IL-13 Protein, P600 Protein, Il-13 Protein, L13 Protein, interleukin 13 Protein, IL13 Protein, Il13 Protein

Hintergrund: Interleukin-13 (IL-13) is a cytokine produced by activated T lymphocytes which regulates the function of mouse macrophages and shares some biological functions with IL-4. Administration of mouse IL-13 to cultured mouse macrophages induces morphological changes (flattening, and development of processes), leads to increases in class II MHC antigen expression, and inhibits the synthesis of several proinflammatory cytokines (including IL-1β, IL-6, TNF, and IL-12). In contrast to human IL-13, mouse IL-13 does not appear to have activity for mouse B cells. Recombinant mouse IL-13 (mIL-13) has multiple glycosylated forms ranging in molecular weights from 11.3 to 15 kD, as detected by SDS-PAGE analysis.

Pathways: JAK-STAT Signalweg, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport