IL12B Protein

Produktnummer ABIN1305061

Produktdetails

Target: IL12B (Interleukin 12 beta (IL12B))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: ELISA

Marke: BD Pharmingen™

Produktmerkmale: Recombinant human IL-12 p40 protein was purified by immunoaffinity chromatography. The recombinant is > 95% pure, as detemined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Aufreinigung: Immunoaffinity Chromatography
Recombinant human IL-12 p40 protein was purified by immunoaffinity chromatography. The recombinant is > 95 % pure, as detemined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.This recombinant protein is routinely tested by ELISA. Other applications were tested during product development only or reported in the literature.

Reinheit: ≥ 95 %

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: The endotoxin level is ≤ 0.1 ng/µg of human IL-12 p40, as measued in a chromogenic LAL assay.

Anwendungsinformationen

Applikationshinweise: ELISA Standard: This recombinant human IL-12 p40 protein is useful as a quantitative standard for an IL-12 p40 sandwich ELISA. For this assay, the purified C8.3 capture antibody (Cat. No. 551227) can be paired with the biotinylated C8.6 detection antibody (Cat. No. 554660).
Note 1: The ELISA antibody pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring human IL-12p40 in serum or plasma our Human IL-12p40 OptEIA™ Set (Cat. No. 555171 ) or OptEIA Kit II (Cat. No. 551116) is specially formulated and recommended.
Note 2: Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

Kommentare:

BD Pharmingen™ Recombinant Human IL-12 p40

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Frozen

Konzentration: 100 μg/mL

Buffer: 100 μg/mL Frozen aqueous buffered solution containing BSA.

Konservierungsmittel: Azide free

Handhabung: Interleukin-12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. The IL-12 p40 monomer shares amino acid sequence homology with the IL-6 receptor. It has been reported that activated PBMC produce a many fold excess of IL-12 p40 monomer over the bioactive p70 heterodimer. The IL-12 p40 monomer has been reported to inhibit binding of IL-12 p70 to the IL-12 receptor, but with 20X less effectiveness than the IL-12 p70 homodimer.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. Recombinant human IL-12 p40 monomer is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution (pH 7.4) and containing at least 1.0 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 1.0 mg/ml carrier protein** such as human or bovine albumin, aliquoted and stored at -80°C. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.

Referenzen

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Ling, Gately, Gubler, Stern, Lin, Hollfelder, Su, Pan, Hakimi: "Human IL-12 p40 homodimer binds to the IL-12 receptor but does not mediate biologic activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 116-27, (1995) (PubMed).

DAndrea, Aste-Amezaga, Valiante, Ma, Kubin, Trinchieri: "Interleukin 10 (IL-10) inhibits human lymphocyte interferon gamma-production by suppressing natural killer cell stimulatory factor/IL-12 synthesis in accessory cells." in: The Journal of experimental medicine, Vol. 178, Issue 3, pp. 1041-8, (1993) (PubMed).

DAndrea, Rengaraju, Valiante, Chehimi, Kubin, Aste, Chan, Kobayashi, Young, Nickbarg: "Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells." in: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1387-98, (1992) (PubMed).

Schoenhaut, Chua, Wolitzky, Quinn, Dwyer, McComas, Familletti, Gately, Gubler: "Cloning and expression of murine IL-12." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3433-40, (1992) (PubMed).

Antigendetails

Target: IL12B (Interleukin 12 beta (IL12B))

Andere Bezeichnung: IL-12 (p40) (IL12B Produkte)

Synonyme: CLMF Protein, CLMF2 Protein, IL-12B Protein, NKSF Protein, NKSF2 Protein, p40 Protein, Il-12b Protein, Il12p40 Protein, Il-12p40 Protein, Il12 Protein, IL-12p40 Protein, IL-12 Protein, IL-12 p40 Protein, il12b Protein, il12p40.b Protein, zgc:152789 Protein, IL-12.p40 Protein, IL12p40 Protein, IL12B Protein, CLMF p40 Protein, interleukin 12B Protein, interleukin 12b Protein, interleukin 12Ba Protein, IL12B Protein, Il12b Protein, il12ba Protein

Hintergrund: Interleukin-12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. The IL-12 p40 monomer shares amino acid sequence homology with the IL-6 receptor. It has been reported that activated PBMC produce a many fold excess of IL-12 p40 monomer over the bioactive p70 heterodimer. The IL-12 p40 monomer has been reported to inhibit binding of IL-12 p70 to the IL-12 receptor, but with 20X less effectiveness than the IL-12 p70 homodimer.

Pathways: JAK-STAT Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation