NMRAL1 Protein (His tag)

Produktnummer ABIN1098305

Produktdetails

Target: NMRAL1 (NmrA-Like Family Domain Containing 1 (NMRAL1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses NMRAL1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Aufreinigung: purified by chromatography

Reinheit: > 95 % by SDS - PAGE

Anwendungsinformationen

Kommentare:

Synonyms: NmrA-like family domain containing 1, HSCARG, SDR48A1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.15 M NaCl

Lagerung: 4 °C

Informationen zur Lagerung: Avoid repeated freezing and thawing cycles.

Antigendetails

Target: NMRAL1 (NmrA-Like Family Domain Containing 1 (NMRAL1))

Andere Bezeichnung: NMRAL1 (NMRAL1 Produkte)

Synonyme: HSCARG Protein, SDR48A1 Protein, 1110025F24Rik Protein, AI256624 Protein, RGD1311451 Protein, NmrA like redox sensor 1 Protein, NmrA-like family domain containing 1 Protein, NMRAL1 Protein, Nmral1 Protein

Hintergrund: NMRAL1 is a redox sensor protein that undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP+ levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. Under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. Recombinant human NMRAL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 35.9kDa(323aa) confirmed by MALDI-TOF

NCBI Accession: NP_065728