S100A5 Protein (His tag)

Produktnummer ABIN1098260

Produktdetails

Target: S100A5 (S100 Calcium Binding Protein A5 (S100A5))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses S100A5 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Aufreinigung: purified by chromatography

Reinheit: > 95 % by SDS - PAGE

Anwendungsinformationen

Kommentare:

Synonyms: S100 calcium binding protein A5, S100D9

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer, pH 8.0, 40% glycerol, 3mM DTT, 200 mM NaCl

Lagerung: 4 °C

Informationen zur Lagerung: Avoid repeated freezing and thawing cycles.

Antigendetails

Target: S100A5 (S100 Calcium Binding Protein A5 (S100A5))

Andere Bezeichnung: S100A5 (S100A5 Produkte)

Synonyme: S100D Protein, S100D9 Protein, S100A5 Protein, S100 calcium binding protein A5 Protein, S100A5 Protein, S100a5 Protein

Hintergrund: S100 calcium binding protein A5, also known as S100A5, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A5 has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. It is expressed in very restricted regions of the adult brain. Recombinant mouse S100A5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 13.4kDa (117aa), confirmed by MALDI-TOF

NCBI Accession: NP_035442

Pathways: S100 Proteine