USP14 Protein (AA 91-494, C-Term) (His tag)

Produktnummer ABIN1097772

Produktdetails

Target: USP14 (Ubiquitin Specific Peptidase 14 (USP14))

Protein-Typ: Recombinant

Proteineigenschaft: AA 91-494, C-Term

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses USP14 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase 14/USP14 (N-6His)

Sequenz: MGSSHHHHHH SSGLVPRGSH MDMTEEQLAS AMELPCGLTN LGNTCYMNAT VQCIRSVPEL KDALKRYAGA LRASGEMASA QYITAALRDL FDSMDKTSSS IPPIILLQFL HMAFPQFAEK GEQGQYLQQD ANECWIQMMR VLQQKLEAIE DDSVKETDSS SASAATPSKK KSLIDQFFGV EFETTMKCTE SEEEEVTKGK ENQLQLSCFI NQEVKYLFTG LKLRLQEEIT KQSPTLQRNA LYIKSSKISR LPAYLTIQMV RFFYKEKESV NAKVLKDVKF PLMLDMYELC TPELQEKMVS FRSKFKDLED KKVNQQPNTS DKKSSPQKEV KYEPFSFADD IGSNNCGYYD LQAVLTHQGR SSSSGHYVSW VKRKQDEWIK FDDDKVSIVT PEDILRLSGG GDWHIAYVLL YGPRRVEIME EESEQ

Produktmerkmale: Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase 14/USP14 is produced by our E. coli expression system. The target protein is expressed with sequence (Asp91-Gln494) of Human USP14.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 20 % Glycerol, pH 8.0.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: -80 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: USP14 (Ubiquitin Specific Peptidase 14 (USP14))

Andere Bezeichnung: usp14 (USP14 Produkte)

Synonyme: TGT Protein, DDBDRAFT_0202821 Protein, DDBDRAFT_0237695 Protein, DDB_0202821 Protein, DDB_0237695 Protein, 2610005K12Rik Protein, 2610037B11Rik Protein, AW107924 Protein, C78769 Protein, ax Protein, zgc:55949 Protein, wu:fk63d09 Protein, MGC81945 Protein, tgt Protein, MGC69343 Protein, USP14 Protein, ubiquitin specific peptidase 14 Protein, peptidase C19 family protein Protein, ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) Protein, ubiquitin specific protease 14 Protein, ubiquitin specific peptidase 14 S homeolog Protein, universal stress protein Protein, Ubiquitin carboxyl-terminal hydrolase 14 Protein, USP14 Protein, usp14 Protein, Usp14 Protein, Bm1_28765 Protein, usp14.S Protein, RR_RS06480 Protein, usp-14 Protein

Hintergrund: Ubiquitin Carboxyl-Terminal Hydrolase 14 (USP14) belongs to the ubiquitin-specific processing (USP) family which is a deubiquitinating enzyme (DUB) with His and Cys domains. USP14 located in the cytoplasm is a proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. USP14 acts also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. In addition, USP14 is indispensable for synaptic development and function at neuromuscular junctions, required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis.
Alternative Names: Ubiquitin Carboxyl-Terminal Hydrolase 14, Deubiquitinating Enzyme 14, Ubiquitin Thioesterase 14, Ubiquitin-Specific-Processing Protease 14, USP14, TGT

Molekulargewicht: 48.45 kDa

UniProt: P54578