SUMO1 Protein (AA 1-101) (His tag)

Produktnummer ABIN1097601

Produktdetails

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-101

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses SUMO1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Small Ubiquitin-Related Modifier 1/SUMO1 (N-6His)

Sequenz: MGSSHHHHHH SSGLVPRGSH MSDQEAKPST EDLGDKKEGE YIKLKVIGQD SSEIHFKVKM TTHLKKLKES YCQRQGVPMN SLRFLFEGQR IADNNTPKEL GMEEEDVIEV YQEQTGGHST V

Produktmerkmale: Recombinant Human Small Ubiquitin-Related Modifier 1/SUMO1 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1­Val101) of Human SUMO1.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 50 mM TrisHCl, 100 mM NaCl, 1 mM DTT, pH 8.5 .

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 3 months

Antigendetails

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Andere Bezeichnung: Small Ubiquitin-Related Modifier 1/SUMO1 (SUMO1 Produkte)

Hintergrund: Small Ubiquitin-Related Modifier 1 (SUMO1) is an Ubiquitin-like protein that belongs to the ubiquitin family with SUMO subfamily. It is a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation. SUMO1 functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. SUMO1 is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. SUMO1 is not active until the last four amino acids of the carboxy-terminus are cleaved off. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins and may also regulate a network of genes involved in palate development.
Alternative Names: Small Ubiquitin-Related Modifier 1, SUMO-1, GAP-Modifying Protein 1, GMP1, SMT3 Homolog 3, Sentrin, Ubiquitin-Homology Domain Protein PIC1, Ubiquitin-Like Protein SMT3C, Smt3C, Ubiquitin-Like Protein UBL1, SUMO1, SMT3C, SMT3H3, UBL1

Molekulargewicht: 13.7 kDa

UniProt: P63165

Pathways: M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway