PCBD1 Protein (AA 2-104) (His tag)

Produktnummer ABIN1097328

Produktdetails

Target: PCBD1 (Pterin-4 alpha-Carbinolamine Dehydratase/dimerization Cofactor of Hepatocyte Nuclear Factor 1 alpha (PCBD1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-104

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PCBD1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Pterin-4-α-Carbinolamine Dehydratase/PHS/PCBD1 (N-6His)

Sequenz: MGSSHHHHHH SSGLVPRGSH MAGKAHRLSA EERDQLLPNL RAVGWNELEG RDAIFKQFHF KDFNRAFGFM TRVALQAEKL DHHPEWFNVY NKVHITLSTH ECAGLSERDI NLASFIEQVA VSMT

Produktmerkmale: Recombinant Human Pterin-4-alpha-Carbinolamine Dehydratase/PCBD1 is produced by our E. coli expression system. The target protein is expressed with sequence (Ala2-Thr104) of Human PCBD1 fused with a His tag at the N-terminus.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 1 mM DTT, pH 8.0.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: -80 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: PCBD1 (Pterin-4 alpha-Carbinolamine Dehydratase/dimerization Cofactor of Hepatocyte Nuclear Factor 1 alpha (PCBD1))

Andere Bezeichnung: PHS/PCBD1 (PCBD1 Produkte)

Synonyme: BcDNA:LP07248 Protein, CG1963 Protein, Dmel\\CG1963 Protein, PCD Protein, pcbd Protein, zgc:73389 Protein, Xdcoh Protein, dcoh Protein, pcd Protein, phs Protein, DDBDRAFT_0204977 Protein, DDBDRAFT_0305045 Protein, DDB_0204977 Protein, DDB_0305045 Protein, Dcoh Protein, Pcbd Protein, DCOH Protein, PHS Protein, PCBD Protein, DCoH Protein, TCF1 Protein, pterin-4a-carbinolamine dehydratase Protein, pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha Protein, pterin-4-alpha-carbinolamine dehydratase Protein, pterin 4 alpha carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) 1 Protein, pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha L homeolog Protein, pterin-4 alpha-carbinolamine dehydratase 1 Protein, Pcd Protein, pcbd1 Protein, Tb11.02.4890 Protein, pcbd Protein, Fbal_2579 Protein, Ftrac_2004 Protein, Celal_2911 Protein, Isop_1133 Protein, Celly_0291 Protein, Pcbd1 Protein, pcbd1.L Protein, PCBD1 Protein

Sub Type: Fusionprotein

Hintergrund: Pterin-4-alpha-Carbinolamine Dehydratase (PCBD1) is the founding member of the Pterin-4-alpha-Carbinolamine Dehydratase Family. PCBD1 is involved in Tetrahydrobiopterin biosynthesis. It seems to prevent the formation of 7-Pterins and accelerate the formation of Quinonoid-BH2. Furthermore, PCBD1 regulates the homodimerization of the transcription factor Hepatocyte Nuclear Factor 1 (HNF1) and enhances its transcriptional activity. Defects in PCBD1 are the cause of BH4-Deficient Hyperphenylalaninemia Type D (HPABH4D). HPABH4D is characterized by the excretion of 7-substituted Pterins in the urine of affected patients.
Alternative Names: Pterin-4-Alpha-Carbinolamine Dehydratase, PHS, 4-Alpha-Hydroxy-Tetrahydropterin Dehydratase, Dimerization Cofactor of Hepatocyte Nuclear Factor 1-Alpha, DCoH, Dimerization Cofactor of HNF1, Phenylalanine Hydroxylase-Stimulating Protein, Pterin Carbinolami

Molekulargewicht: 14.2 kDa

UniProt: P61457