MMP1 Protein (AA 20-469) (His tag)

Produktnummer ABIN1097152

Produktdetails

Target: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 20-469

Spezies: Human

Quelle: Human Cells

Aufreinigungstag / Konjugat: Dieses MMP1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Matrix Metalloproteinase-1/MMP-1 (C-6His)

Sequenz: FPATLETQEQ DVDLVQKYLE KYYNLKNDGR QVEKRRNSGP VVEKLKQMQE FFGLKVTGKP DAETLKVMKQ PRCGVPDVAQ FVLTEGNPRW EQTHLTYRIE NYTPDLPRAD VDHAIEKAFQ LWSNVTPLTF TKVSEGQADI MISFVRGDHR DNSPFDGPGG NLAHAFQPGP GIGGDAHFDE DERWTNNFRE YNLHRVAAHE LGHSLGLSHS TDIGALMYPS YTFSGDVQLA QDDIDGIQAI YGRSQNPVQP IGPQTPKACD SKLTFDAITT IRGEVMFFKD RFYMRTNPFY PEVELNFISV FWPQLPNGLE AAYEFADRDE VRFFKGNKYW AVQGQNVLHG YPKDIYSSFG FPRTVKHIDA ALSEENTGKT YFFVANKYWR YDEYKRSMDP GYPKMIAHDF PGIGHKVDAV FMKDGFFYFF HGTRQYKFDP KTKRILTLQK ANSWFNCRKN VDHHHHHH

Produktmerkmale: Recombinant Human Matrix Metalloproteinase-1/MMP-1 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Phe20-Asn469) of Human MMP-1 fused with a polyhistidine tag at the C-terminus.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM MES, 150 mM NaCl, 0.05 % Brij35, pH 5.5.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: -80 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Andere Bezeichnung: mmp-1 (MMP1 Produkte)

Sub Type: Fusionprotein

Hintergrund: Matrix Metalloproteinase-1 (MMP-1) is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP1 contains several distinct domains: a prodomain that is cleaved upon activation, a catalytic domain containing the zinc binding site, a short hinge region, and a carboxyl terminal (hemopexin like) domain. MMP-1 can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin, alpha1 antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL1, IGFBP3, IGFBP5, pro-MMP2, and pro-MMP9. A significant role of MMP1 is the degradation of fibrillar collagens in extracellular matrix remodeling, characterized by the cleavage of the interstitial collagen triple helix into 3/4, 1/4 fragments. MMP1 may also be involved in enzyme cascades, cytokine regulation and cell surface molecule modulation.
Alternative Names: Interstitial Collagenase, Fibroblast Collagenase, Matrix Metalloproteinase-1, MMP-1, MMP1, CLG

Molekulargewicht: 52.88 kDa

UniProt: P03956