UBE2A Protein (AA 1-152) (GST tag,His tag)

Produktnummer ABIN1096837

Produktdetails

Target: UBE2A (ube2a) (Ubiquitin-Conjugating Enzyme E2A (ube2a))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-152

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses UBE2A Protein ist gelabelt mit GST tag,His tag.

Verwendungszweck: Recombinant Human Ubiquitin-Conjugating Enzyme E2 A/UBE2A (N-GST, 6His)

Sequenz: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGDDDDKSPM GSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED GTFKLTIEFT EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWR DC

Produktmerkmale: Recombinant Human Ubiquitin-Conjugating Enzyme E2 A/UBE2A is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Cys152) of Human UBE2A fused with a GST tag and a His tag at the N-terminus.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10 % Glycerol, pH 7.5.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: -80 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: UBE2A (ube2a) (Ubiquitin-Conjugating Enzyme E2A (ube2a))

Andere Bezeichnung: Ubiquitin-Conjugating Enzyme E2 A/UBE2A (ube2a Produkte)

Synonyme: BHR6A Protein, HR6A Protein, hhr6a Protein, rad6a Protein, ubc2 Protein, mp:zf637-2-000771 Protein, si:bz46j2.4 Protein, wu:fa01h11 Protein, HHR6A Protein, MRXS30 Protein, MRXSN Protein, RAD6A Protein, UBC2 Protein, Mhr6a Protein, ubiquitin conjugating enzyme E2 A Protein, ubiquitin conjugating enzyme E2 A L homeolog Protein, ubiquitin-conjugating enzyme E2A (RAD6 homolog) Protein, ubiquitin-conjugating enzyme E2A Protein, UBE2A Protein, ube2a.L Protein, ube2a Protein, Ube2a Protein

Sub Type: Fusionprotein

Hintergrund: Ubiquitin-Conjugating Enzyme E2 (UBE2A) is a member of the E2 Ubiquitin-Conjugating Enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2A catalyzes the covalent attachment of ubiquitin to other proteins. UBE2A is required for postreplication repair of UV-damaged DNA. UBE2A Interacts with RAD18 and WAC.
Alternative Names: Ubiquitin-Conjugating Enzyme E2 A, RAD6 Homolog A, HR6A, hHR6A, Ubiquitin Carrier Protein A, Ubiquitin-Protein Ligase A, UBE2A, RAD6A

Molekulargewicht: 49 kDa

UniProt: P49459