Recombinant Human EGF-Like Repeat and Discoidin I-Like Domain-Containing Protein 3/EDIL3 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Val17-Glu480) of Human EDIL3 fused with a polyhistidine tag at the C-terminus.
Reinheit
> 95 % as determined by reducing SDS-PAGE.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
Crystallography grade
DEL1
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, ELISA, SDS, Crys
DEL1
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Handhabung
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Lagerung
4 °C/-20 °C/-80 °C
Informationen zur Lagerung
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Haltbarkeit
3 months
Target
EDIL3 (DEL1)
(EGF like repeats and discoidin domains 3 (DEL1))
si:dkey-84i7.1 Protein, Del1 Protein, XDel1 Protein, DEL1 Protein, Del-1 Protein, EGF-like repeats and discoidin I-like domains 3a Protein, EGF like repeats and discoidin domains 3 L homeolog Protein, EGF like repeats and discoidin domains 3 Protein, EGF-like repeats and discoidin I-like domains 3 Protein, edil3a Protein, edil3.L Protein, EDIL3 Protein, Edil3 Protein
Sub Type
Fusionprotein
Hintergrund
EGF-Like Repeat and Discoidin I-Like Domain-Containing Protein 3 (EDIL3) is a 52 kDa extracellular matrix protein that is expressed by endothelial tissues during embryonic vascular development. EDIL3 becomes quiescent at the time of birth, and is no longer expressed in normal adult tissues. EDIL3 has been found to be re-expressed in a number of human tumors as well as in ischemic muscles and ischemic brain tissue, which may play an important role in adult angiogenesis. EDIL3 promotes adherence and migration of endothelial cells, and acts as an endothelial cell survival agent through upregulation of Bcl-2 expression. EDIL3 has also been shown to be an endogenous inhibitor of inflammatory cell recruitment by interfering with the integrin LFA-1-dependent leukocyte-endothelial adhesion. Human EDIL3 is synthesized as a precursor with a 16 amino acid signal sequence and a 464 amino acid mature chain. Alternative Names: EGF-Like Repeats and Discoidin I-Like Domains 3, EDIL3