Carboxypeptidase A2 Protein (AA 17-417) (His tag)

Produktnummer ABIN1096221

Produktdetails

Target: Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 17-417

Spezies: Human

Quelle: Human Cells

Aufreinigungstag / Konjugat: Dieses Carboxypeptidase A2 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Carboxypeptidase A2/CPA2 (C-6His)

Sequenz: LETFVGDQVL EIVPSNEEQI KNLLQLEAQE HLQLDFWKSP TTPGETAHVR VPFVNVQAVK VFLGSQGIAY SIMIEDVQVL LDKENEEMLF NRRRERSGNF NFGAYHTLEE ISQEMDNLVA EHPGLVSKVN IGSSFENRPM NVLKFSTGGD KPAIWLDAGI HAREWVTQAT ALWTANKIVS DYGKDPSITS ILDALDIFLL PVTNPDGYVF SQTKNRMWRK TRSKVSGSLC VGVDPNRNWD AGFGGPGASS NPCSDSYHGP SANSEVEVKS IVDFIKSHGK VKAFITLHSY SQLLMFPYGY KCTKLDDFDE LSEVAQKAAQ SLRSLHGTKY KVGPICSVIY QASGGSIDWS YDYGIKYSFA FELRDTGRYG FLLPARQILP TAEETWLGLK AIMEHVRDHP YVDHHHHHH

Produktmerkmale:

Recombinant Human Carboxypeptidase A2/CPA2 (C-6His)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 150mm NaCl, pH 7.5.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))

Andere Bezeichnung: Carboxypeptidase A2/CPA2 (CPA2 Produkte)

Synonyme: CPA2 Protein, zgc:92530 Protein, carboxypeptidase A2 Protein, carboxypeptidase A2 (pancreatic) Protein, Carboxypeptidase A2 Protein, carboxypeptidase A2, pancreatic Protein, CPA2 Protein, cpa2 Protein, CpipJ_CPIJ001743 Protein, cbpa2 Protein, Cpa2 Protein

Hintergrund: Recombinant Human Carboxypeptidase A2/CPA2 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Ser115-Tyr417) of Human CPA2 fused with a polyhistidine tag at the C-terminus.
Carboxypeptidase A2 (CPA) is a secreted pancreatic procarboxy-peptidase that cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic AAs in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition.

Molekulargewicht: 45.91 kDa

UniProt: P48052