ATG10 Protein (AA 1-190) (His tag,T7 tag)

Produktnummer ABIN1096062

Dieses Recombinant ATG10-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für ATG10 Protein (AA 1-190) (His tag,T7 tag) (ABIN1096062)

Target: ATG10 (Autophagy Related 10 (ATG10))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 1-190

Aufreinigungstag / Konjugat: Dieses ATG10 Protein ist gelabelt mit His tag,T7 tag.

Verwendungszweck: Recombinant Human Autophagy Related 10 Homolog/ATG10 (C-6His, N-T7 tag)

Sequenz: MASMTGGQQM GRGSMEEDEF IGEKTFQRYC AEFIKHSQQI GDSWEWRPSK DCSDGYMCKI HFQIKNGSVM SHLGASTHGQ TCLPMEEAFE LPLDDCEVIE TAAASEVIKY EYHVLYSCSY QVPVLYFRAS FLDGRPLTLK DIWEGVHECY KMRLLQGPWD TITQQEHPIL GQPFFVLHPC KTNEFMTPVL KNSQKINKNV NYITLEHHHH HH

Produktmerkmale:

Recombinant Human Autophagy Related 10 Homolog/ATG10 (C-6His, N-T7 tag)

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 8.0.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: -80 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: ATG10 (Autophagy Related 10 (ATG10))

Andere Bezeichnung: Ubiquitin-Like-Conjugating Enzyme ATG10/ATG10

Hintergrund: Recombinant Human Ubiquitin-Like-Conjugating Enzyme ATG10/ATG10 produced by E. coli expression system. The target protein is expressed with sequence (Met1-Thr190) of Human ATG10 fused with a 6His tag at the C-terminus.
Ubiquitin-Like-Conjugating Enzyme ATG10 (ATG10) is a ubiquitous 28 kDa member of the ATG10 family protein. ATG10 acts as an E2-like enzyme, catalyzes the transfer of ATG12 to ATG5 during in the initial stages of autophagesome formation. The heterodimer of ATG5 and ATG12 subsequntly associates non-covalently with an ATG16 multimer to generate an antophagosome. ATG10 plays a role in the conjugation of ATG12 to ATG5 by interaction with MAP1LC3A. In addition, ATG10 can diretly interact with ATG5 or ATG7.

Molekulargewicht: 24.5 kDa

UniProt: Q9H0Y0

Pathways: ER-Nucleus Signaling, Autophagie