reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20/-80°C
Buffer
the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Lagerung
-20 °C
Rogowsky, Powell, Shirasu, Lin, Morel, Zyprian, Steck, Kado: "Molecular characterization of the vir regulon of Agrobacterium tumefaciens: complete nucleotide sequence and gene organization of the 28.63-kbp regulon cloned as a single unit." in: Plasmid, Vol. 23, Issue 2, pp. 85-106, (1990) (PubMed).
Hagiya, Close, Tait, Kado: "Identification of pTiC58 plasmid-encoded proteins for virulence in Agrobacterium tumefaciens." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 9, pp. 2669-73, (1985) (PubMed).