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General |
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Antigen | Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antikörper |
Epitop | AA 511-525 Alternativen |
Reaktivität | Fledermaus, Huhn, Rind (Kuh), Hund, Ziege, Hamster, Pferd, Human, Affe, Maus, Schwein, Kaninchen, Ratte (Rattus) Alternativen |
Wirt | Kaninchen Alternativen |
Klonalität | |
Konjugat | Dieser RPS6KB1 Antikörper ist unkonjugiert Alternativen |
Applikation |
Immunoprecipitation (IP), Western Blotting (WB)
Alternativen
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Hersteller | Anmelden zum Anzeigen |
Produktdetails anti-RPS6KB1 AntikörperTarget Details RPS6KB1 Anwendungsinformationen Handhabung Bilder |
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Spezifität | Recognizes rat p70 S6 kinase at 64kD. Species cross-reactivity: human and mouse. |
Homologie | Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%) Xenopus (93%) Stickleback, Zebrafish (87%). |
Reinigung | Protein A purified |
Immunogen |
16 residue synthetic peptide (C-FPMISKRPEHLRMNL) corresponding to residues 511-525 of rat S6 kinase. The immunizing peptide is identical to the human and rabbit sequence. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%), Xenopus (93%), Stickleback, Zebrafish (87%). Type of Immunogen: Synthetic peptide |
Isotyp | IgG |
Target Details RPS6KB1Produktdetails anti-RPS6KB1 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben |
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Antigen | |
Andere Bezeichnung | RPS6KB1 / P70S6K / S6K (RPS6KB1 Antibody Abstract) |
Hintergrund |
Name/Gene ID: RPS6KB1 Subfamily: P70 RSK Family: Protein Kinase Synonyms: RPS6KB1, 70 kDa S6 kinase, p70 S6KA, p70-alpha, p70-S6K, p70 S6 kinase alpha, p70s6 kinase, S6K-beta-1, STK14A, p70 ribosomal S6 kinase, p70 S6 kinase, alpha 1, p70 S6 kinase, alpha 2, p70 S6K-alpha, p70-S6K 1, p70S6K, p70S6K1, PS6K, Ribosomal protein S6 kinase I, S6K, S6K1, p70 ribosomal S6 kinase alpha, p70(S6K)-alpha |
Gen-ID | 6198 |
UniProt | P23443 |
Pathways | PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma |
AnwendungsinformationenProduktdetails anti-RPS6KB1 Antikörper Target Details RPS6KB1 Handhabung Bilder zurück nach oben |
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Applikationshinweise |
Approved: IP, WB (0.5 - 2 μg/mL) Usage: Suitable for use in Western Blot, Immunoprecipitation. Western Blot: 0.5-2.0 μg/mL detected S6 Kinase in RIPA lysates from mouse 3T3 cells. Additional bands above 64kD may be observed at higher concentrations of the antibody. Previous lots detected S6 Kinase in RIPA lysates from human A431 and rat L6 cells. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-p70 S6 kinase (1 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: Not recommended. |
Beschränkungen | Nur für Forschungszwecke einsetzbar |
HandhabungProduktdetails anti-RPS6KB1 Antikörper Target Details RPS6KB1 Anwendungsinformationen Bilder zurück nach oben |
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Format | Liquid |
Konzentration | Lot specific |
Buffer | 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol. |
Konservierungsmittel | Sodium azide |
Vorsichtsmaßnahmen | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |