anti-Human PDPK1 Antikörper für Enzyme Immunoassay

Recommended PDPK1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antikörper
  • pdpk1
  • MGC82080
  • PDK1
  • PDPK2
  • PRO0461
  • Pdk1
  • ATP5A
  • si:zfos-979f1.3
  • 1210
  • BG02759
  • CG1201
  • CG1210
  • DSTPK61
  • Dmel\\CG1210
  • Dstpk61
  • PDK
  • PDK-1
  • PDK1/Pk61C
  • PK61C
  • Pk61C
  • Pk61C/PDK1
  • dPDK-1
  • dPDK1
  • dSTPK61
  • pk61c
  • 3'-phosphoinositide-dependent protein kinase 1
  • 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1
  • ATPDK1
  • AtPDK1
  • T32M21.110
  • T32M21_110
  • 3-phosphoinositide dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase 1 L homeolog
  • 3-phosphoinositide dependent protein kinase-1
  • ATP synthase, H+ transporting, mitochondrial Fo complex subunit C2 (subunit 9)
  • 3-phosphoinositide-dependent protein kinase 1
  • pyruvate dehydrogenase kinase, isozyme 1
  • pyruvate dehydrogenase kinase 1
  • Phosphoinositide-dependent kinase 1
  • 3'-phosphoinositide-dependent protein kinase 1
  • PDPK1
  • pdpk1.L
  • pdpk1
  • Pdpk1
  • ATP5G2
  • LOC100380750
  • pdk1
  • PDK1
  • Pdk1
  • pdk-1
Epitop
pSer396
58
32
28
27
26
26
16
15
15
15
13
13
8
7
7
6
6
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
464
237
230
21
13
12
11
9
6
5
4
3
2
1
1
1
1
1
1
Wirt
Kaninchen
385
73
17
Klonalität
Polyklonal
Konjugat
Dieser PDPK1 Antikörper ist unkonjugiert
18
17
15
11
11
7
7
7
7
7
7
7
7
7
5
5
4
3
3
3
3
3
3
2
2
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA)
321
171
130
91
65
65
44
35
26
11
10
5
3
2
1
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN358390
$ 390.50
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN359095 EIA WB Rabbit Ig Fraction N-Term Anmelden zum Anzeigen Polyclonal 3
1 ABIN954076 EIA IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN1108608 EIA FACS IF IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen 4A11 3
1 ABIN303441 EIA IHC (p) Rabbit AA 236-248, pSer241 Anmelden zum Anzeigen Polyclonal 0
1 ABIN2728601 EIA Rabbit IgG Anmelden zum Anzeigen Polyclonal 0

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antikörper
Epitop pSer396
(58), (32), (28), (27), (26), (26), (16), (15), (15), (15), (13), (13), (8), (7), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(464), (237), (230), (21), (13), (12), (11), (9), (6), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Wirt Kaninchen
(385), (73), (17)
Klonalität
Polyklonal
Konjugat Dieser PDPK1 Antikörper ist unkonjugiert
(18), (17), (15), (11), (11), (7), (7), (7), (7), (7), (7), (7), (7), (7), (5), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Applikation Enzyme Immunoassay (EIA)
(321), (171), (130), (91), (65), (65), (44), (35), (26), (11), (10), (5), (3), (2), (1), (1)
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-PDPK1 Antikörper

Target Details PDPK1 Anwendungsinformationen Handhabung Referenzen für anti-PDPK1 Antikörper (ABIN358390) Bilder
Spezifität This antibody detects PDPK1 pSer396.
Reinigung Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S396 of human PDPK1.
Isotyp Ig Fraction
Plasmids, Primers & others

Target Details PDPK1

Produktdetails anti-PDPK1 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-PDPK1 Antikörper (ABIN358390) Bilder zurück nach oben
Antigen
Andere Bezeichnung PDPK1 (PDPK1 Antibody Abstract)
Hintergrund PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.Synonyms: 3-phosphoinositide-dependent proteinkinase 1, PDK-1, PDK1, PDPK1, hPDK1
Molekulargewicht 63151 Da
Gen-ID 5170, 9606
UniProt O15530
Pathways PI3K-Akt Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Anwendungsinformationen

Produktdetails anti-PDPK1 Antikörper Target Details PDPK1 Handhabung Referenzen für anti-PDPK1 Antikörper (ABIN358390) Bilder zurück nach oben
Applikations-hinweise ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-PDPK1 Antikörper Target Details PDPK1 Anwendungsinformationen Referenzen für anti-PDPK1 Antikörper (ABIN358390) Bilder zurück nach oben
Format Liquid
Konzentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Referenzen für anti-PDPK1 Antikörper (ABIN358390)

Produktdetails anti-PDPK1 Antikörper Target Details PDPK1 Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Nilsen, Slagsvold, Skjerpen, Brech, Stenmark, Olsnes: "Peroxisomal targeting as a tool for assaying potein-protein interactions in the living cell: cytokine-independent survival kinase (CISK) binds PDK-1 in vivo in a phosphorylation-dependent manner." in: The Journal of biological chemistry, Vol. 279, Issue 6, pp. 4794-801, 2004

Collins, Deak, Arthur, Armit, Alessi: "In vivo role of the PIF-binding docking site of PDK1 defined by knock-in mutation." in: The EMBO journal, Vol. 22, Issue 16, pp. 4202-11, 2003

Egawa, Maegawa, Shi, Nakamura, Obata, Yoshizaki, Morino, Shimizu, Nishio, Suzuki, Kashiwagi et al.: "Membrane localization of 3-phosphoinositide-dependent protein kinase-1 stimulates activities of Akt and atypical protein kinase C but does not stimulate glucose transport and glycogen synthesis in ..." in: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38863-9, 2002

Sato, Fujita, Tsuruo: "Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39360-7, 2002

Scott, Ingram, Ball: "PDK1-dependent activation of atypical PKC leads to degradation of the p21 tumour modifier protein." in: The EMBO journal, Vol. 21, Issue 24, pp. 6771-80, 2002

Bilder

Produktdetails anti-PDPK1 Antikörper Target Details PDPK1 Anwendungsinformationen Handhabung Referenzen für anti-PDPK1 Antikörper (ABIN358390) zurück nach oben
Bilder des Herstellers
 image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer396) antibody (ABIN358390) anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer396) antibody