When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
ARPC2
(Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))
Synonyme
arc34 Peptide, p34-arc Peptide, pnas-139 Peptide, pro2446 Peptide, Arpc2 Peptide, Arc-p34 Peptide, 2210023N03Rik Peptide, 34kDa Peptide, p34-Arc Peptide, fk84a07 Peptide, wu:fa22f07 Peptide, wu:fk84a07 Peptide, zgc:77769 Peptide, ARC34 Peptide, PNAS-139 Peptide, actin related protein 2/3 complex subunit 2 Peptide, actin related protein 2/3 complex, subunit 2, 34kDa Peptide, actin related protein 2/3 complex, subunit 2 Peptide, actin related protein 2/3 complex subunit 2 S homeolog Peptide, ARPC2 Peptide, arpc2 Peptide, Arpc2 Peptide, arpc2.S Peptide