Human CXCL16 EZ Set™ ELISA Kit (DIY Antibody Pairs)
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- Target Alle CXCL16 Produkte
- CXCL16 (Chemokine (C-X-C Motif) Ligand 16 (CXCL16))
- Bindungsspezifität
- AA 49-137
- Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Wirt
- Kaninchen
- Methodentyp
- Sandwich ELISA
- Applikation
- ELISA
- Verwendungszweck
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects CXCL16 in Human samples.
- Marke
- EZ-Set™
- Spezifität
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Expression system for standard: E.coli
Immunogen sequence: N49-P137 - Kreuzreaktivität (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Produktmerkmale
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Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.
Tissue Specificity: Expressed in T-cell areas. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow.
- Bestandteile
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
- Benötigtes Material
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1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
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- Kommentare
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The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
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Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. - Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
- Haltbarkeit
- 12 months
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- Target
- CXCL16 (Chemokine (C-X-C Motif) Ligand 16 (CXCL16))
- Andere Bezeichnung
- CXCL16 (CXCL16 Produkte)
- Synonyme
- CXCL16 Matched Antibody Pair, 0910001K24Rik Matched Antibody Pair, AV290116 Matched Antibody Pair, BB024863 Matched Antibody Pair, CXCL16v1 Matched Antibody Pair, CXCL16v2 Matched Antibody Pair, SR-PSOX Matched Antibody Pair, Zmynd15 Matched Antibody Pair, b2b498Clo Matched Antibody Pair, CXCLG16 Matched Antibody Pair, SRPSOX Matched Antibody Pair, C-X-C motif chemokine ligand 16 Matched Antibody Pair, chemokine (C-X-C motif) ligand 16 Matched Antibody Pair, CXCL16 Matched Antibody Pair, Cxcl16 Matched Antibody Pair
- Hintergrund
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Protein Function: Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. .
Background: Chemokine(C-X-C motif) ligand 16(CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines(with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T(NKT) cells. CXCL16 interacts with the chemokine receptorCXCR6, also known as Bonzo. The novel CXC-chemokine ligand 16(CXCL16) functions as transmembrane adhesion molecule on the surface of APCs and as a soluble chemoattractant for activated T cells. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The gene for human CXCL16 is located on chromosome 17.
Synonyms: C-X-C motif chemokine 16,Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein,SR-PSOX,Small-inducible cytokine B16,Transmembrane chemokine CXCL16,CXCL16,SCYB16, SRPSOX,UNQ2759/PRO6714,
Full Gene Name: C-X-C motif chemokine 16
Cellular Localisation: Cell membrane, Single-pass type I membrane protein . Secreted. Also exists as a soluble form. - UniProt
- Q9H2A7
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