Human HPRG / HRG ELISA Pair Set

Details zu Produkt Nr. ABIN2212586, Anbieter: Anmelden zum Anzeigen
Antigen
  • GDF-15
  • MIC-1
  • MIC1
  • NAG-1
  • PDF
  • PLAB
  • PTGFB
  • 6030402G23Rik
  • ARIA
  • D230005F13Rik
  • GGF
  • GGFII
  • HRG
  • HRGalpha
  • Hgl
  • NDF
  • SMDF
  • GGF2
  • HGL
  • HRG1
  • HRGA
  • MST131
  • NRG-1
  • heregulin
  • nrg1-A
  • pro-neuregulin-1
  • NRG1
  • wu:fm70e01
  • DKFZp459A1838
  • AI265597
  • AW413091
  • D16jh2
  • D18020
  • Hprg
  • Hrgp
  • growth differentiation factor 15
  • neuregulin 1
  • neuregulin 1 L homeolog
  • histidine-rich glycoprotein
  • GDF15
  • Nrg1
  • NRG1
  • nrg1.L
  • nrg1
  • Hrg
Reaktivität
Human
Wirt
Maus
Konjugat
HRP
Methodentyp
Sandwich ELISA
Applikation
ELISA
Optionen
Hersteller
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Verwendungszweck The human HPRG / HRG ELISA Pair Set is for the quantitative determination of human HPRG / HRG.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
Nachweismethode Colorimetric
Sensitivität 18.75 pg/mL
Produktmerkmale Capture Antibody: 0.4 mg/mL of mouse anti-HPRG monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating.

Detection Antibody: 0.2 mg/mL rabbit anti-HPRG polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-protector, pH 7.4). Dilute to working concentration of 0.7 μg/mL in detection antibody dilution buffer before use.

Standard: Each vial contains 10 ng of recombinant HPRG. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 °C to -80 °C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1.2 ng/mL is recommended.

Sensitivity: The minimum detectable dose of human HPRG / HRG was determined to be approximately 18.75 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Bestandteile
  • Capture Ab
  • Detection Ab
  • Standard
Andere Bezeichnung HRG
Applikationshinweise Optimal working dilution should be determined by the investigator.
Protokoll This ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for HPRG / HRGcoated on a 96-well plate. Standards and samples are added to the wells, andany HPRG / HRG present binds to the immobilized antibody. The wells are washedand a horseradish peroxidase conjugated rabbit anti-HPRG / HRG polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". Thewells are again washed and TMB substrate solution is loaded, which producescolor in proportion to the amount of HPRG / HRG present in the sample. To endthe enzyme reaction, the stop solution is added and absorbances of themicrowell are read at 450 nm.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung 4 °C,-20 °C,-80 °C
Informationen zur Lagerung Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!

Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
Bilder des Herstellers
ELISA image for Human HPRG / HRG ELISA Pair Set (ABIN2212586) Human HPRG / HRG ELISA Pair Set
Produkt verwendet in: Deuschle, Birkel, Hambruch, Hornberger, Kinzel, Perovi?-Ottstadt, Schulz, Hahn, Burnet, Kremoser: "The nuclear bile acid receptor FXR controls the liver derived tumor suppressor histidine-rich glycoprotein." in: International journal of cancer. Journal international du cancer, Vol. 136, Issue 11, pp. 2693-704, 2015 (PubMed).