anti-Human MDM2 Antikörper für Immunofluorescence

Recommended MDM2 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) Antikörper
  • ACTFS
  • HDMX
  • hdm2
  • zgc:109834
  • 1700007J15Rik
  • AA415488
  • Mdm-2
  • MDM2 proto-oncogene
  • MDM2 proto-oncogene S homeolog
  • Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse)
  • MDM2 oncogene, E3 ubiquitin protein ligase
  • transformed mouse 3T3 cell double minute 2
  • MDM2
  • mdm2.S
  • Mdm2
  • mdm2
Epitop
Center
45
42
36
29
26
24
18
15
15
15
12
8
8
7
7
6
5
5
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
397
153
112
14
8
6
4
2
2
2
2
2
1
1
1
1
Wirt
Kaninchen
326
75
14
2
Klonalität
Polyklonal
Konjugat
Dieser MDM2 Antikörper ist unkonjugiert
13
13
13
10
10
10
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
285
142
110
83
70
40
39
24
12
12
12
12
4
4
2
2
1
1
1
1
Optionen
Hersteller
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Western Blotting (WB) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782)
Immunohistochemistry (IHC) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782)
Immunofluorescence (IF) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782)
Western Blotting (WB) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782)
Western Blotting (WB) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782)
Chromatin Immunoprecipitation (ChIP) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782)
Produktnummer ABIN2854782
$ 358.60
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
11.720334 ABIN2704976 IC IF IHC WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0
11.720334 ABIN2706519 IC IF IP IHC WB Rabbit pSer166 Anmelden zum Anzeigen Polyclonal 0
11.720334 ABIN2957662 IC IF IP IHC WB Rabbit Anmelden zum Anzeigen Polyclonal 0
11.720334 ABIN1532360 IF ELISA WB Rabbit IgG AA 132-181 Anmelden zum Anzeigen Polyclonal 0
11.720334 ABIN1532743 IF IHC ELISA Rabbit IgG AA 151-200 Anmelden zum Anzeigen Polyclonal 0
11.720334 ABIN1533341 IF IHC ELISA WB Rabbit IgG AA 391-440 Anmelden zum Anzeigen Polyclonal 0
11.720334 ABIN1531340 IF IHC ELISA WB Rabbit IgG AA 132-181, pSer166 Anmelden zum Anzeigen Polyclonal 0
11.720334 ABIN1531714 IF ELISA Rabbit IgG AA 151-200, pSer186 Anmelden zum Anzeigen Polyclonal 0
11.720334 ABIN388067 IF IHC (p) WB Rabbit Ig Fraction AA 141-176, Ser166 Anmelden zum Anzeigen Polyclonal 2
11.720334 ABIN1870422 IF IHC WB Rabbit IgG pSer166 Anmelden zum Anzeigen Polyclonal 0
11.720334 ABIN1870425 IF IHC WB Rabbit IgG pSer166 Anmelden zum Anzeigen Polyclonal 0
11.720334 ABIN2854822 ICC IF IHC (p) Rabbit IgG Center Anmelden zum Anzeigen Polyclonal 0
11.720334 ABIN2425119 IF IHC WB Rabbit IgG pSer166 Anmelden zum Anzeigen Polyclonal 0
11.720334 ABIN3031734 IF IHC ELISA WB Rabbit Ig Fraction AA 141-176 Anmelden zum Anzeigen Polyclonal 0
11.720334 ABIN3020052 IF IHC WB Rabbit pSer166 Anmelden zum Anzeigen Polyclonal 0
1 ABIN401610 IF IHC (p) WB Rabbit pSer166 Anmelden zum Anzeigen Polyclonal 3
1 ABIN3185503 ELISA IF IHC WB Rabbit IgG Ser880 Anmelden zum Anzeigen Polyclonal 0
1 ABIN319417 IF IHC (p) WB Rabbit Ser166 Anmelden zum Anzeigen Polyclonal 3
1 ABIN363126 IF IHC Rabbit IgG AA 164-168 Anmelden zum Anzeigen Polyclonal 5
1 ABIN362482 IF IHC WB Rabbit IgG pSer166 Anmelden zum Anzeigen Polyclonal 5

General

Antigen Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) Antikörper
Epitop Center
(45), (42), (36), (29), (26), (24), (18), (15), (15), (15), (12), (8), (8), (7), (7), (6), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus
(397), (153), (112), (14), (8), (6), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Wirt Kaninchen
(326), (75), (14), (2)
Klonalität
Polyklonal
Konjugat Dieser MDM2 Antikörper ist unkonjugiert
(13), (13), (13), (10), (10), (10), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(285), (142), (110), (83), (70), (40), (39), (24), (12), (12), (12), (12), (4), (4), (2), (2), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-MDM2 Antikörper

Target Details MDM2 Anwendungsinformationen Handhabung Bilder
Kreuzreaktivität Katze, Hund, Schwein
Kreuzreaktivität (Details) Cat (95 %), Dog (91 %), Pig (93 %)
Produktmerkmale Rabbit Polyclonal antibody to MDM2 (Mdm2 p53 binding protein homolog)
MDM2 antibody
Reinigung Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human MDM2. The exact sequence is proprietary.
Isotyp IgG

Target Details MDM2

Produktdetails anti-MDM2 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung MDM2 (MDM2 Antibody Abstract)
Hintergrund This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.

Cellular Localization: Nucleus , nucleoplasm , Cytoplasm , nucleolus
Molekulargewicht 55 kDa
Gen-ID 4193
Pathways p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Autophagie

Anwendungsinformationen

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Handhabung Bilder zurück nach oben
Applikationshinweise Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:1000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:1000*
Kommentare

Positive Control: H1299 , HCT116 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , 293T , mouse MDM2-transfected 293T
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 0.74 mg/mL
Buffer 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Konservierungsmittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Bilder

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) WB Image MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 μg 293T w...
Immunohistochemistry (IHC) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) IHC-P Image Immunohistochemical analysis of paraffin-embedded human lung cancer, usin...
Immunofluorescence (IF) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MDM2, ...
Western Blotting (WB) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) WB Image MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 μg Neuro2...
Western Blotting (WB) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) WB Image MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 μg H1299 ...
Chromatin Immunoprecipitation (ChIP) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) ChIP Image Cross-linked ChIP was performed with PC-3 chromatin extract and 5 μg of ei...
Immunoprecipitation (IP) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) IP Image MDM2 antibody immunoprecipitates MDM2 protein in IP experiments. IP samples:...