CHEK1 Proteine (CHEK1)

Human Checkpoint Kinase 1 (CHEK1) Interaktionspartner

1. inhibition of Chk1 can potentiate activity of nucleoside analogs in TP53 (zeige TP53 Proteine)-mutated B-lymphoid cells

2. Data show that protein phosphatase-1 (zeige PPP1CB Proteine) alpha (PP1alpha (zeige PPP1CA Proteine)) is required to maintain checkpoint kinase 1 (CHK1) in a dephosphorylated state and for the accelerated replication fork progression in Spi1 (zeige SPI1 Proteine)/PU.1 transcription factor-overexpressing cells.

3. Following DNA damage, addition of the TLK1 (zeige TLK1 Proteine) inhibitor, THD, or overexpression of NEK1 (zeige NEK1 Proteine)-T141A mutant impaired ATR (zeige ANTXR1 Proteine) and Chk1 activation, indicating the existence of a TLK1 (zeige TLK1 Proteine)>NEK1 (zeige NEK1 Proteine)>ATR (zeige ANTXR1 Proteine)>Chk1 pathway. Indeed, overexpression of the NEK1 (zeige NEK1 Proteine)-T141A mutant resulted in an altered cell cycle response after exposure of cells to oxidative stress, including bypass of G1 arrest and implementation of an intra S-phase checkpoint.

4. Suggest that activation of ATR (zeige ANTXR1 Proteine)/CHK1 signaling pathway is key for Epstein Barr virus-induced B-cell transformation.

5. Histone deacetylases 1 and 2 cooperate in regulating BRCA1, CHK1, and RAD51 (zeige RAD51 Proteine) expression in acute myeloid leukemia (zeige BCL11A Proteine) cells.

6. Chk1 inhibition with GDC (zeige SLC25A16 Proteine)-0425 in combination with gemcitabine was tolerated with manageable bone marrow suppression. The observed preliminary clinical activity warrants further investigation of this chemopotentiation strategy

7. Data show that the checkpoint kinase 1/2 (Chk1/Chk2 (zeige CHEK2 Proteine)) inhibitor prexasertib (LY2606368) inhibits cell viability in B-/T-ALL cell lines.

8. we demonstrate that CHK1 mRNA is overexpressed in two independent cohorts of medulloblastoma patient samples in comparison to normal cerebellum

9. results suggest a Chk1-OGT (zeige OGT Proteine)-vimentin (zeige VIM Proteine) pathway that regulates the intermediate filament network during cytokinesis

10. CHEK1-mediated DNA damage checkpoint has a role in the ESR2 (zeige ESR2 Proteine)-NCF1 (zeige NCF1 Proteine)-ROS (zeige ROS1 Proteine) pathway sensitization of esophageal cancer cells to 5-fluorouracil-induced cell death

Xenopus laevis Checkpoint Kinase 1 (CHEK1) Interaktionspartner

1. Inhibition of Drf1 (zeige DBF4B Proteine) is the primary mechanism by which Chk1 blocks cell-cycle progression in the early embryo and is an essential function of Chk1 at the blastula-to-gastrula stage of development.

2. The study analyzes the role of Chk1 in the replication program in sperm nuclei replicating in Xenopus egg extracts by a combination of experimental and modelling approaches.

3. ATR-Chk1 DDR pathway appears to be dispensable for the preferential association of REV1 to MMC-damaged chromatin.

4. Results show that Chk1 negatively regulates the Treslin-mediated loading of Cdc45 (zeige CDC45 Proteine) onto chromatin and thereby serves to antagonize the initiation of replication.

5. The MRN complex, in particular the nuclease (zeige DCLRE1C Proteine) activity of Mre11 (zeige MRE11A Proteine), plays an important role in the activation of Chk1 in response to stalled replication forks.

6. DNA polymerase kappa (zeige POLK Proteine)-dependent DNA synthesis at stalled replication forks is important for CHK1 activation.

7. APE2 (zeige APEX2 Proteine) associates with Chk1; a serine residue (S86) in the Chk1-binding motif of APE2 (zeige APEX2 Proteine) is essential for Chk1 phosphorylation, indicating a Claspin-like but distinct role for APE2 (zeige APEX2 Proteine) in ATR-Chk1 signaling.

8. Data show that the death pathway is independent of ERK (zeige MAPK1 Proteine) but relies on activating Bad phosphorylation through the control of both kinases Cdk1 (zeige CDK1 Proteine) and JNK (zeige MAPK8 Proteine).

9. Findings reveal that XH2AX has a specific role in anterior neural formation of Xenopus, which is mediated through phosphorylation of XH2AX at Thr (zeige TRH Proteine)(16) by Chk1.

10. mechanism of Chk1 activation at the DNA replication checkpoint

Mouse (Murine) Checkpoint Kinase 1 (CHEK1) Interaktionspartner

1. During neurogenesis, cortical neurons became protected from S-phase Chk1 pathway activation by the DNA methyltransferase (zeige DNMT1 Proteine) Dnmt1 (zeige DNMT1 Proteine), and underwent cell death after S-phase progression.

2. work reveals that simulated microgravity promotes the apoptotic response through a combined modulation of the Uev1A/TICAM/TRAF (zeige TRAF1 Proteine)/NF-kappaB (zeige NFKB1 Proteine)-regulated apoptosis and the p53 (zeige TP53 Proteine)/PCNA (zeige PCNA Proteine)- and ATM (zeige ATM Proteine)/ATR-Chk1/2-controlled DNA-damage response pathways.

3. Chk1(-/-) MEFs exhibited the absence of double-strand breaks, yet cells showed delayed DNA damage recovery with pan-nuclear immunostaining pattern of Histone H2AX.

4. RAD9 (zeige RAD9A Proteine) has a prominent role in the ATR-Chk1 pathway that is necessary for successful formation of the damage-sensing complex and DNA damage checkpoint signaling.

5. Acute inactivation of E4F1 (zeige E4F1 Proteine) in these cells results in CHK1-dependent checkpoint deficiency and multiple mitochondrial dysfunctions that lead to increased ROS (zeige ROS1 Proteine) production, energy stress, and inhibition of de novo pyrimidine synthesis

6. Altogether, our results classify E4F1 (zeige E4F1 Proteine) as a master regulator of CHK1 activity that ensures high fidelity of DNA replication, thus safeguarding genome stability.

7. Heterozygous loss of Chk1 in a Wnt (zeige WNT2 Proteine)-driven background resulted in an increase in DNA damage and apoptosis and accelerated both tumour development and progression.

8. PAR, supplied by PARP1, interacts with Chk1 via a novel PAR-binding regulatory (PbR) motif in Chk1, independent of ATR and its activity

9. ATM (zeige ATM Proteine) (pSer-1981)-Chk1 (pSer-345) cascade might have mediated G2/M cell cycle arrest to allowed time to facilitate sperm-derived DNA damage repair in mouse zygotes fertilized with oxygen-stressed sperm.

10. High level of phospho-Chk1 is associated with skin tumors.

Cow (Bovine) Checkpoint Kinase 1 (CHEK1) Interaktionspartner

1. The Chk1 directly phosphorylates eNOS (zeige NOS3 Proteine) Ser (zeige SIGLEC1 Proteine)(1179) in response to UV irradiation, which is dependent on Hsp90 (zeige HSP90 Proteine) interaction.