anti-Ratte (Rattus) CHEK1 Antikörper für Western Blotting

Recommended CHEK1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Checkpoint Kinase 1 (CHEK1) Antikörper
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CAMK/CAMKL/CHK1 protein kinase Chk1
  • checkpoint kinase 1 S homeolog
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1.S
  • Chek1
  • chek1
Epitop
pSer317
73
57
41
36
28
17
16
15
14
13
7
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
513
211
179
14
14
10
10
10
7
5
5
5
4
2
1
1
Wirt
Kaninchen
453
54
22
7
Klonalität
Polyklonal
Konjugat
Dieser CHEK1 Antikörper ist unkonjugiert
14
13
12
11
10
8
3
3
3
3
3
3
3
3
3
3
3
2
1
1
Applikation
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
446
220
171
80
71
40
32
21
21
16
13
7
6
6
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN6255201
$ 390.77
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
24.303753 ABIN6255160 ELISA ICC IF IHC WB Rabbit IgG pSer280 Anmelden zum Anzeigen Polyclonal 1
20.056355 ABIN634171 IHC WB Rabbit Anmelden zum Anzeigen Polyclonal 0
19.803753 ABIN2779737 IHC WB Rabbit Middle Region Anmelden zum Anzeigen Polyclonal 1
19.803753 ABIN6256251 ELISA ICC IF IHC WB Rabbit IgG pSer296 Anmelden zum Anzeigen Polyclonal 0
19.803753 ABIN6256255 ELISA ICC IF IHC WB Rabbit IgG pSer301 Anmelden zum Anzeigen Polyclonal 0
19.803753 ABIN6255167 ELISA ICC IF IHC WB Rabbit IgG pSer286 Anmelden zum Anzeigen Polyclonal 0
17.056355 ABIN3181961 ELISA IHC WB Rabbit IgG pSer317 Anmelden zum Anzeigen Polyclonal 0
17.056355 ABIN3182434 ELISA IF WB Rabbit IgG pSer301 Anmelden zum Anzeigen Polyclonal 0
17.056355 ABIN3183900 ELISA IF WB Rabbit IgG Ser2481 Anmelden zum Anzeigen Polyclonal 0
17.056355 ABIN3183903 ELISA IHC WB Rabbit IgG Ser249 Anmelden zum Anzeigen Polyclonal 0
17.056355 ABIN3183901 ELISA IF IHC WB Rabbit IgG Ser2484 Anmelden zum Anzeigen Polyclonal 0
17.056355 ABIN634170 WB Rabbit Anmelden zum Anzeigen Polyclonal 0
16.252602 ABIN1589998 ELISA WB Goat Internal Region Anmelden zum Anzeigen Polyclonal 0
14.5 ABIN6260808 ELISA ICC IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 1
13.803753 ABIN3049475 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
11.5 ABIN757072 IF (p) IHC (p) WB Rabbit IgG AA 280-330, pSer317 Anmelden zum Anzeigen Polyclonal 1
10.803753 ABIN2705867 IC IF IHC WB Rabbit Center Anmelden zum Anzeigen Polyclonal 0
10.803753 ABIN2705868 IC IF IHC WB Rabbit Center Anmelden zum Anzeigen Polyclonal 0
10.803753 ABIN2972901 IF/ICC IHC WB KLH Rabbit IgG Center, pSer345 Anmelden zum Anzeigen Polyclonal 0
10.803753 ABIN6571262 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0

General

Antigen Checkpoint Kinase 1 (CHEK1) Antikörper
Epitop pSer317
(73), (57), (41), (36), (28), (17), (16), (15), (14), (13), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(513), (211), (179), (14), (14), (10), (10), (10), (7), (5), (5), (5), (4), (2), (1), (1)
Wirt Kaninchen
(453), (54), (22), (7)
Klonalität
Polyklonal
Konjugat Dieser CHEK1 Antikörper ist unkonjugiert
(14), (13), (12), (11), (10), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1)
Applikation ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(446), (220), (171), (80), (71), (40), (32), (21), (21), (16), (13), (7), (6), (6)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-CHEK1 Antikörper

Target Details CHEK1 Anwendungsinformationen Handhabung Referenzen für anti-CHEK1 Antikörper (ABIN6255201) Bilder
Spezifität Phospho-Chk1 (Ser317) Antibody detects endogenous levels of Chk1 only when phosphorylated at Serine 317
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human Chk1 around the phosphorylation site of Serine 317
Isotyp IgG
Plasmids, Primers & others

Target Details CHEK1

Produktdetails anti-CHEK1 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-CHEK1 Antikörper (ABIN6255201) Bilder zurück nach oben
Antigen
Andere Bezeichnung Chk1 (CHEK1 Antibody Abstract)
Hintergrund

Description: Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a role in replication fork maintenance through regulation of PCNA. May regulate the transcription of genes that regulate cell-cycle progression through the phosphorylation of histones. Phosphorylates histone H3.1 (to form H3T11ph), which leads to epigenetic inhibition of a subset of genes. May also phosphorylate RB1 to promote its interaction with the E2F family of transcription factors and subsequent cell cycle arrest.

Gene: CHEK1

Molekulargewicht 56kDa
Gen-ID 1111
UniProt O14757
Pathways p53 Signalweg, Apoptose, Zellzyklus

Anwendungsinformationen

Produktdetails anti-CHEK1 Antikörper Target Details CHEK1 Handhabung Referenzen für anti-CHEK1 Antikörper (ABIN6255201) Bilder zurück nach oben
Applikations-hinweise WB 1:500-1:2000 IHC 1:50-1:500, IF/ICC 1:100-1:500
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-CHEK1 Antikörper Target Details CHEK1 Anwendungsinformationen Referenzen für anti-CHEK1 Antikörper (ABIN6255201) Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months

Referenzen für anti-CHEK1 Antikörper (ABIN6255201)

Produktdetails anti-CHEK1 Antikörper Target Details CHEK1 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Jiang, Wang, Xing, Shen, Lian, Yi, Zhang, Yang, Liu, Zhang: "Sterigmatocystin-induced checkpoint adaptation depends on Chk1 in immortalized human gastric epithelial cells in vitro." in: Archives of toxicology, Vol. 91, Issue 1, pp. 259-270, 2017

Bilder

Produktdetails anti-CHEK1 Antikörper Target Details CHEK1 Anwendungsinformationen Handhabung Referenzen für anti-CHEK1 Antikörper (ABIN6255201) zurück nach oben
Bilder des Herstellers
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) ABIN6267224 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized...
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) Western blot analysis of Chk1 phosphorylation expression in MCF7 whole cell lysates,T...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) ABIN6267224 at 1/100 staining mouse spleen tissue sections by IHC-P. The tissue was f...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) ABIN6267224 at 1/100 staining mouse brain tissue sections by IHC-P. The tissue was fo...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) ABIN6267224 at 1/100 staining human vascular cancer tissue sections by IHC-P. The tis...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) ABIN6267224 at 1/100 staining human lymphoid tissue sections by IHC-P. The tissue was...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) ABIN6267224 at 1/100 staining human appendiceal tissue sections by IHC-P. The tissue ...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) ABIN6267224 at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was for...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) ABIN6267224 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was form...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody (ABIN6255201) ABIN6267224 at 1/100 staining rat spleen tissue sections by IHC-P. The tissue was for...