anti-Maus CHEK1 Antikörper für Western Blotting

Recommended CHEK1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Checkpoint Kinase 1 (CHEK1) Antikörper
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CAMK/CAMKL/CHK1 protein kinase Chk1
  • checkpoint kinase 1 S homeolog
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1.S
  • Chek1
  • chek1
Epitop
Internal Region, pSer345
73
57
40
35
28
17
16
15
14
12
7
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
503
210
179
14
14
10
10
10
7
5
4
4
4
2
1
1
Wirt
Kaninchen
445
51
22
6
Klonalität
Polyklonal
Konjugat
Dieser CHEK1 Antikörper ist unkonjugiert
14
13
12
11
10
8
3
3
3
3
3
3
3
3
3
3
3
2
1
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
434
216
171
80
68
40
32
21
18
16
13
7
6
6
Optionen
Hersteller
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Bilder

Produktnummer ABIN2857048
$ 466.18
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
24.302582 ABIN6255160 ELISA ICC IF IHC WB Rabbit IgG pSer280 Anmelden zum Anzeigen Polyclonal 1
24.302582 ABIN6255201 ELISA ICC IF IHC WB Rabbit IgG pSer317 Anmelden zum Anzeigen Polyclonal 1
24.057352 ABIN969476 ELISA WB Mouse IgG1 Anmelden zum Anzeigen 2G1D5 2
19.802582 ABIN2779737 IHC WB Rabbit Middle Region Anmelden zum Anzeigen Polyclonal 1
19.802582 ABIN6256251 ELISA ICC IF IHC WB Rabbit IgG pSer296 Anmelden zum Anzeigen Polyclonal 0
19.802582 ABIN6255167 ELISA ICC IF IHC WB Rabbit IgG pSer286 Anmelden zum Anzeigen Polyclonal 0
19.802582 ABIN6256255 ELISA ICC IF IHC WB Rabbit IgG pSer301 Anmelden zum Anzeigen Polyclonal 0
19.557352 ABIN3209997 ELISA WB Mouse IgG1 Anmelden zum Anzeigen 2G1D5 0
19.557352 ABIN634171 IHC WB Rabbit Anmelden zum Anzeigen Polyclonal 0
16.557352 ABIN3181961 ELISA IHC WB Rabbit IgG pSer317 Anmelden zum Anzeigen Polyclonal 0
16.557352 ABIN3182434 ELISA IF WB Rabbit IgG pSer301 Anmelden zum Anzeigen Polyclonal 0
16.557352 ABIN3183900 ELISA IF WB Rabbit IgG Ser2481 Anmelden zum Anzeigen Polyclonal 0
16.557352 ABIN3183901 ELISA IF IHC WB Rabbit IgG Ser2484 Anmelden zum Anzeigen Polyclonal 0
16.557352 ABIN3183903 ELISA IHC WB Rabbit IgG Ser249 Anmelden zum Anzeigen Polyclonal 0
16.557352 ABIN634170 WB Rabbit Anmelden zum Anzeigen Polyclonal 0
14.5 ABIN6260808 ELISA ICC IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 1
13.802582 ABIN6260806 ELISA ICC IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
13.802582 ABIN3049475 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
12.754771 ABIN4901262 ELISA WB Mouse IgG1 Anmelden zum Anzeigen 2G1D5 0
12.754771 ABIN1842465 IHC WB Rabbit IgG pSer317 Anmelden zum Anzeigen Polyclonal 0

General

Antigen Checkpoint Kinase 1 (CHEK1) Antikörper
Epitop Internal Region, pSer345
(73), (57), (40), (35), (28), (17), (16), (15), (14), (12), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus
(503), (210), (179), (14), (14), (10), (10), (10), (7), (5), (4), (4), (4), (2), (1), (1)
Wirt Kaninchen
(445), (51), (22), (6)
Klonalität
Polyklonal
Konjugat Dieser CHEK1 Antikörper ist unkonjugiert
(14), (13), (12), (11), (10), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1)
Applikation Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(434), (216), (171), (80), (68), (40), (32), (21), (18), (16), (13), (7), (6), (6)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-CHEK1 Antikörper

Target Details CHEK1 Anwendungsinformationen Handhabung Bilder
Kreuzreaktivität Rind (Kuh), Ratte (Rattus), Xenopus laevis, Zebrafisch (Danio rerio)
Kreuzreaktivität (Details) Rat (100 %), Zebrafish (88 %), Xenopus laevis (88 %), Bovine (100 %)
Produktmerkmale Rabbit Polyclonal antibody to Chk1 (phospho Ser345) (CHK1 checkpoint homolog (S. pombe))
Chk1 (phospho Ser345) antibody [C1C2], Internal
Reinigung Purified by antigen-affinity chromatography.
Isotyp IgG
Plasmids, Primers & others

Target Details CHEK1

Produktdetails anti-CHEK1 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Chk1 (CHEK1 Antibody Abstract)
Hintergrund Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.

Cellular Localization: Nucleus , Cytoplasm
Molekulargewicht 54 kDa
Gen-ID 1111
Pathways p53 Signalweg, Apoptose, Zellzyklus

Anwendungsinformationen

Produktdetails anti-CHEK1 Antikörper Target Details CHEK1 Handhabung Bilder zurück nach oben
Applikations-hinweise Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Kommentare

Positive Control: HCT116 , HCT116 cells with 30uM cisplatin treatment for 24hr

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-CHEK1 Antikörper Target Details CHEK1 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Konservierungs-mittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Bilder

Produktdetails anti-CHEK1 Antikörper Target Details CHEK1 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Checkpoint Kinase 1 (CHEK1) (Internal Region), (pSer345) antibody (ABIN2857048) ICC/IF Image Chk1 (phospho Ser345) antibody [C1C2], Internal detects Chk1 (phospho Se...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (Internal Region), (pSer345) antibody (ABIN2857048) IHC-P Image Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using C...
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (Internal Region), (pSer345) antibody (ABIN2857048) WB Image Sample: 20 ug of HCT116 P53 whole cell lysate 10% SDS PAGE Chk1-phopho-S345 ...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 1 (CHEK1) (Internal Region), (pSer345) antibody (ABIN2857048) IHC-P Image Chk1 (phospho Ser345) antibody [C1C2], Internal detects Chk1 (phospho Ser...