anti-Human ATM Antikörper für Immunofluorescence

Recommended ATM Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Ataxia Telangiectasia Mutated (ATM) Antikörper
  • ATM
  • Atm
  • CG6535
  • Dmel\\CG6535
  • Tefu
  • atm
  • atm/tefu
  • dATM
  • tef
  • Xatm
  • at1
  • atdc
  • tel1
  • telo1
  • AT1
  • ATA
  • ATC
  • ATD
  • ATDC
  • ATE
  • TEL1
  • TELO1
  • AI256621
  • C030026E19Rik
  • telomere fusion
  • ATM serine/threonine kinase L homeolog
  • ATM serine/threonine kinase
  • ataxia telangiectasia mutated
  • ataxia telangiectasia mutated (atm)
  • serine/threonine-protein kinase ATM
  • tefu
  • atm.L
  • atm
  • ATM
  • EDI_100660
  • CpipJ_CPIJ001772
  • BDBG_08252
  • PAAG_02532
  • MCYG_05088
  • VDBG_06833
  • ACLA_015700
  • LOC5565620
  • MGYG_07634
  • PGTG_14279
  • Atm
Epitop
C-Term
89
34
17
16
16
15
8
8
6
6
6
6
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
352
154
78
27
8
1
1
1
1
Wirt
Kaninchen
225
100
17
9
3
Klonalität
Polyklonal
Konjugat
Dieser ATM Antikörper ist unkonjugiert
12
11
9
8
8
6
5
5
5
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Applikation
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
216
120
110
85
81
64
53
43
27
22
13
6
4
4
2
2
2
1
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN151030
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
11.720334 ABIN151622 ICC IF IHC IP WB Mouse IgG1 Anmelden zum Anzeigen 5C2 26
11.720334 ABIN2668604 ICC IF Mouse IgG1 kappa pSer1981 Anmelden zum Anzeigen 10H11-E-12 3
11.720334 ABIN1869997 IF IHC WB Rabbit IgG pSer1981 Anmelden zum Anzeigen Polyclonal 0
11.720334 ABIN389888 DB IF Rabbit Ig Fraction pSer1981 Anmelden zum Anzeigen Polyclonal 2
11.720334 ABIN513576 IF ELISA Mouse IgG2a kappa AA 1-138, full length Anmelden zum Anzeigen 2C10 0
11.720334 ABIN5075177 ICC IF Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
11.720334 ABIN3030033 DB IF ELISA Rabbit Ig Fraction pSer1981 Anmelden zum Anzeigen Polyclonal 0
1 ABIN151775 ChIP ELISA FACS ICC IF IHC IHC (p) IP WB Mouse IgG1 full length Anmelden zum Anzeigen 2C1 114
1 ABIN151772 ICC IF IHC (p) IP IHC WB Mouse IgG1 kappa pSer1981 Anmelden zum Anzeigen 10H11-E12 20
1 ABIN319313 IF WB Rabbit Ser1981 Anmelden zum Anzeigen Polyclonal 4
1 ABIN152102 ICC IF IHC (p) IP IHC PLA WB Rabbit AA 2550-2600 Anmelden zum Anzeigen Polyclonal 2
1 ABIN363380 ICC FACS IF IP IHC ELISA WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN362751 IF WB Rabbit IgG AA 1979-1983 Anmelden zum Anzeigen Polyclonal 5
1 ABIN1882297 IF WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN1721771 ICC IF IHC WB Mouse IgG1 kappa pSer1981 Anmelden zum Anzeigen 10H11-E12 0
1 ABIN5689237 IF WB Rabbit AA 1979-1983 Anmelden zum Anzeigen Polyclonal 0
1 ABIN4282187 ELISA FACS ICC IF APC Mouse IgG1 full length Anmelden zum Anzeigen 2C1 0
1 ABIN5499237 IF IHC (p) IP WB Mouse IgG1 AA 2577-3056 Anmelden zum Anzeigen 2C1 0
1 ABIN2882455 IF IHC WB Rabbit IgG pSer1981 Anmelden zum Anzeigen Polyclonal 0
1 ABIN4282171 ICC IF IHC IHC (p) WB DyLight 488 Mouse IgG1 kappa pSer1981 Anmelden zum Anzeigen 10H11-E12 0

General

Antigen Ataxia Telangiectasia Mutated (ATM) Antikörper
Epitop C-Term
(89), (34), (17), (16), (16), (15), (8), (8), (6), (6), (6), (6), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus
(352), (154), (78), (27), (8), (1), (1), (1), (1)
Wirt Kaninchen
(225), (100), (17), (9), (3)
Klonalität
Polyklonal
Konjugat Dieser ATM Antikörper ist unkonjugiert
(12), (11), (9), (8), (8), (6), (5), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Applikation Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(216), (120), (110), (85), (81), (64), (53), (43), (27), (22), (13), (6), (4), (4), (2), (2), (2), (1), (1)
Pubmed 77 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-ATM Antikörper

Target Details ATM Anwendungsinformationen Handhabung Referenzen für anti-ATM Antikörper (ABIN151030) Bilder
Reinigung Immunogen affinity purified
Immunogen A fragment of the human ATM protein corresponding to the C-terminus (within the last third of the protein sequence). [UniProt# Q13315]

Target Details ATM

Produktdetails anti-ATM Antikörper Anwendungsinformationen Handhabung Referenzen für anti-ATM Antikörper (ABIN151030) Bilder zurück nach oben
Antigen
Andere Bezeichnung ATM (ATM Antibody Abstract)
Hintergrund Gene Symbol: ATM
Molekulargewicht Theoretical MW: 350 kDa
Gen-ID 472
UniProt Q13315
Pathways p53 Signalweg, Apoptose, DNA Reparatur, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus

Anwendungsinformationen

Produktdetails anti-ATM Antikörper Target Details ATM Handhabung Referenzen für anti-ATM Antikörper (ABIN151030) Bilder zurück nach oben
Applikationshinweise Western Blot 1:500-1:1000, Flow Cytometry, ELISA, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100This ATM antibody can be used for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, it detects a band at ~350 kDa, representing ATM. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Immunohistochemistry-FFPE sections protocol specific for ATM Antibody Immunohistochemistry- FFPE sectionsDeparaffinization
1. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.
. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.Quench Endogenous Peroxidase
. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation.
. Place slides in distilled water: 2 changes for 2 minutes each.Retrieve Epitopes
. Preheat Citrate
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-ATM Antikörper Target Details ATM Anwendungsinformationen Referenzen für anti-ATM Antikörper (ABIN151030) Bilder zurück nach oben
Format Liquid
Konzentration 2.5 mg/mL
Buffer PBS and 50 % Glycerol
Buffer contains: 0.05 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung -80 °C,-20 °C
Informationen zur Lagerung Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Referenzen für anti-ATM Antikörper (ABIN151030)

Produktdetails anti-ATM Antikörper Target Details ATM Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Murata, Hashimoto, Urushihara, Shiga, Takeda, Jingu, Hosoi: "Knockdown of AMPKα decreases ATM expression and increases radiosensitivity under hypoxia and nutrient starvation in an SV40-transformed human fibroblast cell line, LM217." in: Biochemical and biophysical research communications, Vol. 495, Issue 4, pp. 2566-2572, 2018 Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

Li, Li, Kong, Yan, Ahmad, Liu: "Plk1 Phosphorylation of Mre11 Antagonizes the DNA Damage Response." in: Cancer research, Vol. 77, Issue 12, pp. 3169-3180, 2017 Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

Wu, Wang, Chen, Chao, Chang, Chen, Hung: "Early Passage Mesenchymal Stem Cells Display Decreased Radiosensitivity and Increased DNA Repair Activity." in: Stem cells translational medicine, Vol. 6, Issue 6, pp. 1504-1514, 2017 Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

Qi, Qiu, Gu, Tian, Zhang: "ATM mediates spermidine-induced mitophagy via PINK1 and Parkin regulation in human fibroblasts." in: Scientific reports, Vol. 6, pp. 24700, 2016 (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence

Nagy, Kalousi, Furst, Koch, Fischer, Soutoglou: "Tankyrases Promote Homologous Recombination and Check Point Activation in Response to DSBs." in: PLoS genetics, Vol. 12, Issue 2, pp. e1005791, 2016 Von den Autoren verwendete Methode: Western Blotting (WB)

Parplys, Zhao, Sharma, Groesser, Liang, Maranon, Leung, Grundt, Dray, Idate, Østvold, Schild, Sung, Wiese: "NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability." in: Nucleic acids research, Vol. 43, Issue 20, pp. 9817-34, 2015 (Probematerial (Species): Human). Weitere Details: Western Blotting

Murata, Uehara, Hosoi: "Activation of mTORC1 under nutrient starvation conditions increases cellular radiosensitivity in human liver cancer cell lines, HepG2 and HuH6." in: Biochemical and biophysical research communications, Vol. 468, Issue 4, pp. 684-90, 2015 (Probematerial (Species): Human). Weitere Details: Western Blotting

Yoon, Ishdorj, Graham, Johnston, Gibson: "Valproic acid enhances fludarabine-induced apoptosis mediated by ROS and involving decreased AKT and ATM activation in B-cell-lymphoid neoplastic cells." in: Apoptosis : an international journal on programmed cell death, Vol. 19, Issue 1, pp. 191-200, 2014 (Probematerial (Species): Human). Weitere Details: Western Blotting

Morita, Tanimoto, Murakami, Morinaga, Hosoi: "Mitochondria are required for ATM activation by extranuclear oxidative stress in cultured human hepatoblastoma cell line Hep G2 cells." in: Biochemical and biophysical research communications, Vol. 443, Issue 4, pp. 1286-90, 2014 (Probematerial (Species): Human). Weitere Details: Western Blotting

Silva, Stambaugh, Yokomori, Shah, Berns: "DNA damage to a single chromosome end delays anaphase onset." in: The Journal of biological chemistry, Vol. 289, Issue 33, pp. 22771-84, 2014

Brinley, Theriot, Nelman-Gonzalez, Crucian, Stowe, Barrett, Pierson: "Characterization of Epstein-Barr virus reactivation in a modeled spaceflight system." in: Journal of cellular biochemistry, Vol. 114, Issue 3, pp. 616-24, 2013 (Probematerial (Species): Human). Weitere Details: Flow Cytometry

Gautam, Bridge: "The kinase activity of ataxia-telangiectasia mutated interferes with adenovirus E4 mutant DNA replication." in: Journal of virology, Vol. 87, Issue 15, pp. 8687-96, 2013 (Probematerial (Species): Human). Weitere Details: Western Blotting

Rankin, Sarkar, Faller: "Mechanism of T-oligo-induced cell cycle arrest in Mia-PaCa pancreatic cancer cells." in: Journal of cellular physiology, Vol. 227, Issue 6, pp. 2586-94, 2012 (Probematerial (Species): Human). Weitere Details: Western Blotting

Jacquemin, Rieunier, Jacob, Bellanger, dEnghien, Laugé, Stoppa-Lyonnet, Stern: "Underexpression and abnormal localization of ATM products in ataxia telangiectasia patients bearing ATM missense mutations." in: European journal of human genetics : EJHG, Vol. 20, Issue 3, pp. 305-12, 2012 (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence

Nguyen, Possemato, Bauerlein, Xie, Scully, Hahn: "Nek4 regulates entry into replicative senescence and the response to DNA damage in human fibroblasts." in: Molecular and cellular biology, Vol. 32, Issue 19, pp. 3963-77, 2012 (Probematerial (Species): Human). Weitere Details: Western Blotting

Xie, Li, Xing, Tian, Yin, He, Zhang: "ATM-mediated NuSAP phosphorylation induces mitotic arrest." in: Biochemical and biophysical research communications, Vol. 404, Issue 1, pp. 413-8, 2011 (Probematerial (Species): Human). Weitere Details: Western Blotting

Wang, Wu, Wei, Hong, Chiang: "Inactivation of ataxia telangiectasia mutated gene can increase intracellular reactive oxygen species levels and alter radiation-induced cell death pathways in human glioma cells." in: International journal of radiation biology, Vol. 87, Issue 4, pp. 432-42, 2011 (Probematerial (Species): Human). Weitere Details: Flow Cytometry

Dixon, Henry, Siroky, Chu, Groen, Bissler: "Cell cycle control and DNA damage response of conditionally immortalized urothelial cells." in: PLoS ONE, Vol. 6, Issue 1, pp. e16595, 2011 (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Du, Kayali, Bertoni, Fike, Hu, Iversen, Gatti: "Arginine-rich cell-penetrating peptide dramatically enhances AMO-mediated ATM aberrant splicing correction and enables delivery to brain and cerebellum." in: Human molecular genetics, Vol. 20, Issue 16, pp. 3151-60, 2011 (Probematerial (Species): Human). Weitere Details: Western Blotting

Yang, Chou, Pan, Chen, Tsou, Yeh, Cheung, Chen, Chang: "Enhancement of non-homologous end joining DNA repair capacity confers cancer cells resistance to the novel selenophene compound, D-501036." in: Cancer letters, Vol. 309, Issue 1, pp. 110-8, 2011 (Probematerial (Species): Human). Weitere Details: Western Blotting

Bilder

Produktdetails anti-ATM Antikörper Target Details ATM Anwendungsinformationen Handhabung Referenzen für anti-ATM Antikörper (ABIN151030) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Western Blot: ATM Antibody [ABIN151030] - Detection of ATM in HeLa whole cell lysate ...
Immunofluorescence (IF) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Immunohistochemical staining using ABIN151030.
Western Blotting (WB) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Detection of ATM in HeLa whole cell lysate using ABIN151030. (30 second exposure)
Western Blotting (WB) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Western Blot: ATM Antibody [ABIN151030] - Detection of ATM in Raji whole cell lysate.
Flow Cytometry (FACS) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Flow Cytometry: ATM Antibody [ABIN151030] - Analysis of PE conjugate of ABIN151030. A...
Immunohistochemistry (IHC) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Immunohistochemistry: ATM Antibody [ABIN151030] - Staining of Testis, Seminiferous Tu...
Immunofluorescence (IF) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Immunocytochemistry/Immunofluorescence: ATM Antibody [ABIN151030] - HeLa cells were f...