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Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validierenRelevance Score | ABIN | Application | Konjugat | Host | Isotype | Epitope | Hersteller | Clonality | References | Details |
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11.720334 | ABIN151622 | ICC IF IHC IP WB | Mouse | IgG1 | Anmelden zum Anzeigen | 5C2 | 26 | |||
11.720334 | ABIN2668604 | ICC IF | Mouse | IgG1 kappa | pSer1981 | Anmelden zum Anzeigen | 10H11-E-12 | 3 | ||
11.720334 | ABIN1869997 | IF IHC WB | Rabbit | IgG | pSer1981 | Anmelden zum Anzeigen | Polyclonal | 0 | ||
11.720334 | ABIN389888 | DB IF | Rabbit | Ig Fraction | pSer1981 | Anmelden zum Anzeigen | Polyclonal | 2 | ||
11.720334 | ABIN513576 | IF ELISA | Mouse | IgG2a kappa | AA 1-138, full length | Anmelden zum Anzeigen | 2C10 | 0 | ||
11.720334 | ABIN5075177 | ICC IF | Rabbit | IgG | Anmelden zum Anzeigen | Polyclonal | 0 | |||
11.720334 | ABIN3030033 | DB IF ELISA | Rabbit | Ig Fraction | pSer1981 | Anmelden zum Anzeigen | Polyclonal | 0 | ||
1 | ABIN151775 | ChIP ELISA FACS ICC IF IHC IHC (p) IP WB | Mouse | IgG1 | full length | Anmelden zum Anzeigen | 2C1 | 114 | ||
1 | ABIN151772 | ICC IF IHC (p) IP IHC WB | Mouse | IgG1 kappa | pSer1981 | Anmelden zum Anzeigen | 10H11-E12 | 20 | ||
1 | ABIN319313 | IF WB | Rabbit | Ser1981 | Anmelden zum Anzeigen | Polyclonal | 4 | |||
1 | ABIN152102 | ICC IF IHC (p) IP IHC PLA WB | Rabbit | AA 2550-2600 | Anmelden zum Anzeigen | Polyclonal | 2 | |||
1 | ABIN363380 | ICC FACS IF IP IHC ELISA WB | Rabbit | C-Term | Anmelden zum Anzeigen | Polyclonal | 0 | |||
1 | ABIN362751 | IF WB | Rabbit | IgG | AA 1979-1983 | Anmelden zum Anzeigen | Polyclonal | 5 | ||
1 | ABIN1882297 | IF WB | Rabbit | IgG | Anmelden zum Anzeigen | Polyclonal | 0 | |||
1 | ABIN1721771 | ICC IF IHC WB | Mouse | IgG1 kappa | pSer1981 | Anmelden zum Anzeigen | 10H11-E12 | 0 | ||
1 | ABIN5689237 | IF WB | Rabbit | AA 1979-1983 | Anmelden zum Anzeigen | Polyclonal | 0 | |||
1 | ABIN4282187 | ELISA FACS ICC IF | APC | Mouse | IgG1 | full length | Anmelden zum Anzeigen | 2C1 | 0 | |
1 | ABIN5499237 | IF IHC (p) IP WB | Mouse | IgG1 | AA 2577-3056 | Anmelden zum Anzeigen | 2C1 | 0 | ||
1 | ABIN2882455 | IF IHC WB | Rabbit | IgG | pSer1981 | Anmelden zum Anzeigen | Polyclonal | 0 | ||
1 | ABIN4282171 | ICC IF IHC IHC (p) WB | DyLight 488 | Mouse | IgG1 kappa | pSer1981 | Anmelden zum Anzeigen | 10H11-E12 | 0 |
General |
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Antigen | Ataxia Telangiectasia Mutated (ATM) Antikörper |
Epitop | C-Term Alternativen |
Reaktivität | Human, Maus Alternativen |
Wirt | Kaninchen Alternativen |
Klonalität | |
Konjugat | Dieser ATM Antikörper ist unkonjugiert Alternativen |
Applikation |
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Alternativen
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77 Publikationen vorhanden |
Hersteller | Anmelden zum Anzeigen |
Produktdetails anti-ATM AntikörperTarget Details ATM Anwendungsinformationen Handhabung Referenzen für anti-ATM Antikörper (ABIN151030) Bilder |
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Reinigung | Immunogen affinity purified |
Immunogen | A fragment of the human ATM protein corresponding to the C-terminus (within the last third of the protein sequence). [UniProt# Q13315] |
Target Details ATMProduktdetails anti-ATM Antikörper Anwendungsinformationen Handhabung Referenzen für anti-ATM Antikörper (ABIN151030) Bilder zurück nach oben |
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Antigen | |
Andere Bezeichnung | ATM (ATM Antibody Abstract) |
Hintergrund | Gene Symbol: ATM |
Molekulargewicht | Theoretical MW: 350 kDa |
Gen-ID | 472 |
UniProt | Q13315 |
Pathways | p53 Signalweg, Apoptose, DNA Reparatur, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus |
AnwendungsinformationenProduktdetails anti-ATM Antikörper Target Details ATM Handhabung Referenzen für anti-ATM Antikörper (ABIN151030) Bilder zurück nach oben |
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Applikationshinweise | Western Blot 1:500-1:1000, Flow Cytometry, ELISA, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100This ATM antibody can be used for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, it detects a band at ~350 kDa, representing ATM. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Kommentare |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
Protokoll |
Immunohistochemistry-FFPE sections protocol specific for ATM Antibody Immunohistochemistry- FFPE sectionsDeparaffinization 1. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. . Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.Quench Endogenous Peroxidase . Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation. . Place slides in distilled water: 2 changes for 2 minutes each.Retrieve Epitopes . Preheat Citrate |
Beschränkungen | Nur für Forschungszwecke einsetzbar |
HandhabungProduktdetails anti-ATM Antikörper Target Details ATM Anwendungsinformationen Referenzen für anti-ATM Antikörper (ABIN151030) Bilder zurück nach oben |
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Format | Liquid |
Konzentration | 2.5 mg/mL |
Buffer |
PBS and 50 % Glycerol Buffer contains: 0.05 % Sodium Azide |
Konservierungsmittel | Sodium azide |
Vorsichtsmaßnahmen | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handhabung | Avoid freeze-thaw cycles |
Lagerung | -80 °C,-20 °C |
Informationen zur Lagerung | Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles. |
Referenzen für anti-ATM Antikörper (ABIN151030)Produktdetails anti-ATM Antikörper Target Details ATM Anwendungsinformationen Handhabung Bilder zurück nach oben |
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Produkt verwendet in: |
Murata, Hashimoto, Urushihara, Shiga, Takeda, Jingu, Hosoi: "Knockdown of AMPKα decreases ATM expression and increases radiosensitivity under hypoxia and nutrient starvation in an SV40-transformed human fibroblast cell line, LM217." in: Biochemical and biophysical research communications, Vol. 495, Issue 4, pp. 2566-2572, 2018 Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human). Li, Li, Kong, Yan, Ahmad, Liu: "Plk1 Phosphorylation of Mre11 Antagonizes the DNA Damage Response." in: Cancer research, Vol. 77, Issue 12, pp. 3169-3180, 2017 Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human). Wu, Wang, Chen, Chao, Chang, Chen, Hung: "Early Passage Mesenchymal Stem Cells Display Decreased Radiosensitivity and Increased DNA Repair Activity." in: Stem cells translational medicine, Vol. 6, Issue 6, pp. 1504-1514, 2017 Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human). Qi, Qiu, Gu, Tian, Zhang: "ATM mediates spermidine-induced mitophagy via PINK1 and Parkin regulation in human fibroblasts." in: Scientific reports, Vol. 6, pp. 24700, 2016 (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence Nagy, Kalousi, Furst, Koch, Fischer, Soutoglou: "Tankyrases Promote Homologous Recombination and Check Point Activation in Response to DSBs." in: PLoS genetics, Vol. 12, Issue 2, pp. e1005791, 2016 Von den Autoren verwendete Methode: Western Blotting (WB) Parplys, Zhao, Sharma, Groesser, Liang, Maranon, Leung, Grundt, Dray, Idate, Østvold, Schild, Sung, Wiese: "NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability." in: Nucleic acids research, Vol. 43, Issue 20, pp. 9817-34, 2015 (Probematerial (Species): Human). Weitere Details: Western Blotting Murata, Uehara, Hosoi: "Activation of mTORC1 under nutrient starvation conditions increases cellular radiosensitivity in human liver cancer cell lines, HepG2 and HuH6." in: Biochemical and biophysical research communications, Vol. 468, Issue 4, pp. 684-90, 2015 (Probematerial (Species): Human). Weitere Details: Western Blotting Yoon, Ishdorj, Graham, Johnston, Gibson: "Valproic acid enhances fludarabine-induced apoptosis mediated by ROS and involving decreased AKT and ATM activation in B-cell-lymphoid neoplastic cells." in: Apoptosis : an international journal on programmed cell death, Vol. 19, Issue 1, pp. 191-200, 2014 (Probematerial (Species): Human). Weitere Details: Western Blotting Morita, Tanimoto, Murakami, Morinaga, Hosoi: "Mitochondria are required for ATM activation by extranuclear oxidative stress in cultured human hepatoblastoma cell line Hep G2 cells." in: Biochemical and biophysical research communications, Vol. 443, Issue 4, pp. 1286-90, 2014 (Probematerial (Species): Human). Weitere Details: Western Blotting Silva, Stambaugh, Yokomori, Shah, Berns: "DNA damage to a single chromosome end delays anaphase onset." in: The Journal of biological chemistry, Vol. 289, Issue 33, pp. 22771-84, 2014 Brinley, Theriot, Nelman-Gonzalez, Crucian, Stowe, Barrett, Pierson: "Characterization of Epstein-Barr virus reactivation in a modeled spaceflight system." in: Journal of cellular biochemistry, Vol. 114, Issue 3, pp. 616-24, 2013 (Probematerial (Species): Human). Weitere Details: Flow Cytometry Gautam, Bridge: "The kinase activity of ataxia-telangiectasia mutated interferes with adenovirus E4 mutant DNA replication." in: Journal of virology, Vol. 87, Issue 15, pp. 8687-96, 2013 (Probematerial (Species): Human). Weitere Details: Western Blotting Rankin, Sarkar, Faller: "Mechanism of T-oligo-induced cell cycle arrest in Mia-PaCa pancreatic cancer cells." in: Journal of cellular physiology, Vol. 227, Issue 6, pp. 2586-94, 2012 (Probematerial (Species): Human). Weitere Details: Western Blotting Jacquemin, Rieunier, Jacob, Bellanger, dEnghien, Laugé, Stoppa-Lyonnet, Stern: "Underexpression and abnormal localization of ATM products in ataxia telangiectasia patients bearing ATM missense mutations." in: European journal of human genetics : EJHG, Vol. 20, Issue 3, pp. 305-12, 2012 (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence Nguyen, Possemato, Bauerlein, Xie, Scully, Hahn: "Nek4 regulates entry into replicative senescence and the response to DNA damage in human fibroblasts." in: Molecular and cellular biology, Vol. 32, Issue 19, pp. 3963-77, 2012 (Probematerial (Species): Human). Weitere Details: Western Blotting Xie, Li, Xing, Tian, Yin, He, Zhang: "ATM-mediated NuSAP phosphorylation induces mitotic arrest." in: Biochemical and biophysical research communications, Vol. 404, Issue 1, pp. 413-8, 2011 (Probematerial (Species): Human). Weitere Details: Western Blotting Wang, Wu, Wei, Hong, Chiang: "Inactivation of ataxia telangiectasia mutated gene can increase intracellular reactive oxygen species levels and alter radiation-induced cell death pathways in human glioma cells." in: International journal of radiation biology, Vol. 87, Issue 4, pp. 432-42, 2011 (Probematerial (Species): Human). Weitere Details: Flow Cytometry Dixon, Henry, Siroky, Chu, Groen, Bissler: "Cell cycle control and DNA damage response of conditionally immortalized urothelial cells." in: PLoS ONE, Vol. 6, Issue 1, pp. e16595, 2011 (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting Du, Kayali, Bertoni, Fike, Hu, Iversen, Gatti: "Arginine-rich cell-penetrating peptide dramatically enhances AMO-mediated ATM aberrant splicing correction and enables delivery to brain and cerebellum." in: Human molecular genetics, Vol. 20, Issue 16, pp. 3151-60, 2011 (Probematerial (Species): Human). Weitere Details: Western Blotting Yang, Chou, Pan, Chen, Tsou, Yeh, Cheung, Chen, Chang: "Enhancement of non-homologous end joining DNA repair capacity confers cancer cells resistance to the novel selenophene compound, D-501036." in: Cancer letters, Vol. 309, Issue 1, pp. 110-8, 2011 (Probematerial (Species): Human). Weitere Details: Western Blotting |
BilderProduktdetails anti-ATM Antikörper Target Details ATM Anwendungsinformationen Handhabung Referenzen für anti-ATM Antikörper (ABIN151030) zurück nach oben |
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Bilder des Herstellers |
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