anti-Human Progesterone Receptor Antikörper für Immunohistochemistry (Formalin-fixed Sections)

Recommended Progesterone Receptor Antibody

Antigen
Progesterone Receptor (PGR) Antikörper
  • NR3C3
  • PR
  • 9930019P03Rik
  • BB114106
  • ENSMUSG00000074510
  • PR-A
  • PR-B
  • XPR-1
  • pgr
  • xpr-2
  • LOC443444
  • PGR
  • pg
  • pr
  • gb:dq017620
  • progesterone receptor
  • progesterone receptor L homeolog
  • PGR
  • Pgr
  • pgr.L
  • pgr
Epitop
AA 412-526
53
48
39
27
25
21
19
18
16
16
15
12
10
10
10
9
9
8
8
8
7
7
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
661
113
110
25
10
9
7
6
5
3
3
3
2
2
1
1
1
1
Wirt
Kaninchen
379
291
3
1
Klonalität
Monoklonal
Konjugat
Dieser Progesterone Receptor Antikörper ist unkonjugiert
28
17
15
12
12
12
11
11
11
11
11
11
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
1
Applikation
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
448
203
163
127
93
66
50
28
26
21
18
9
5
3
2
2
1
1
1
Optionen

Bilder

Produktnummer ABIN1027517
$ 1,643.25
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Clonality References Details
16.49087 ABIN1027518 IHC (f) IHC (p) Rabbit IgG AA 412-526 SP42 0
4 ABIN6167045 IHC (f) APC Mouse IgG1, kappa PR484 0
4 ABIN6167052 IHC (f) PerCP Mouse IgG1, kappa PR484 0
4 ABIN6167053 IHC (f) PE Mouse IgG1, kappa PR484 0
4 ABIN6167128 IHC (f) WB PerCP Mouse IgG1, kappa PR501 0
4 ABIN6167129 IHC (f) WB PE Mouse IgG1, kappa PR501 0
4 ABIN6167083 IHC (f) APC Mouse IgG1, kappa PR500 0
4 ABIN6167090 IHC (f) PerCP Mouse IgG1, kappa PR500 0
4 ABIN6167091 IHC (f) PE Mouse IgG1, kappa PR500 0
4 ABIN6167121 IHC (f) WB APC Mouse IgG1, kappa PR501 0
4 ABIN6167056 IHC (f) Mouse IgG1, kappa PR484 0
4 ABIN6167132 IHC (f) WB Mouse IgG1, kappa PR501 0
4 ABIN6167094 IHC (f) Mouse IgG1, kappa PR500 0
1 ABIN6167019 IHC (f) CF®405S Mouse IgG1, kappa PR484 0
1 ABIN6167021 IHC (f) CF405M Mouse IgG1, kappa PR484 0
1 ABIN6167041 IHC (f) CF®488A Mouse IgG1, kappa PR484 0
1 ABIN6167025 IHC (f) CF®543 Mouse IgG1, kappa PR484 0
1 ABIN6167029 IHC (f) CF®555 Mouse IgG1, kappa PR484 0
1 ABIN6167033 IHC (f) CF®568 Mouse IgG1, kappa PR484 0
1 ABIN6167043 IHC (f) CF®594 Mouse IgG1, kappa PR484 0

General

Antigen Progesterone Receptor (PGR) Antikörper
Epitop AA 412-526
(53), (48), (39), (27), (25), (21), (19), (18), (16), (16), (15), (12), (10), (10), (10), (9), (9), (8), (8), (8), (7), (7), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(661), (113), (110), (25), (10), (9), (7), (6), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Wirt Kaninchen
(379), (291), (3), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser Progesterone Receptor Antikörper ist unkonjugiert
(28), (17), (15), (12), (12), (12), (11), (11), (11), (11), (11), (11), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1)
Applikation Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(448), (203), (163), (127), (93), (66), (50), (28), (26), (21), (18), (9), (5), (3), (2), (2), (1), (1), (1)
Hersteller

Produktdetails anti-Progesterone Receptor Antikörper

Target Details Progesterone Receptor Anwendungsinformationen Handhabung Bilder
Verwendungszweck This antibody is designed for the specific localization of human PR using IHC techniques in formalin-fixed, paraffin- embedded tissue sections.
Produktmerkmale The determination of steroid hormone receptors is of great importance for therapy of patients with hormone receptor expressing tumours. Progesterone and estrogen receptor positive breast carcinoma patients have demonstrated a better response to endocrine therapy than receptor negative patients. Expression of progesterone receptors in tumour cells of human breast carcinoma points to an intact estrogen receptor and to sensitivity of this tumour to endocrine therapy. Several investigations have shown that progesterone receptor is a better prognostic marker than estrogen receptor. Immunohistochemistry (IHC) is a complex technique in which immunological and histological detection methods are combined. In general, the manipulation and processing of tissues before immunostaining, especially different types of tissue fixation and embedding, as well as the nature of the tissues themselves may cause inconsistent results (Nadji and Morales, 1983). Endogenous pseudoperoxidase and peroxidase activity or endogenous biotin and alkaline phosphatase activity can cause non-specific staining results depending on the detection system used. Tissues that contain Hepatitis B surface antigen (HBsAg) can produce false positives when using HRP detection systems (Omata et al, 1980). Insufficient contrast staining and/or improper mounting of the sample may influence the interpretation of results.
Reinigung Ready-to-use, Prediluted, obtained from culture supernatant
Benötigtes Material All reagents, materials, and laboratory equipment for IHC procedures are not provided with this antibody. This includes adhesive slides and cover slips, positive and negative control tissues, Xylene or adequate substitute, ethanol, distilled H2O, heat pretreatment equipment (pressure cooker, steamer, microwave), pipettes, Coplin jars, glass jars, moist chamber, histological baths, negative control reagents, counter-staining solution, mounting materials, and microscope.
Immunogen Recombinant protein corresponding to amino acid residues 412-526 of human PR.
Klon SP42
Isotyp IgG
Plasmids, Primers & others

Target Details Progesterone Receptor

Produktdetails anti-Progesterone Receptor Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Progesterone Receptor (PGR Antibody Abstract)
Pathways Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Smooth Muscle Cell Migration

Anwendungsinformationen

Produktdetails anti-Progesterone Receptor Antikörper Target Details Progesterone Receptor Handhabung Bilder zurück nach oben
Kommentare

Staining pattern: Nuclear. The interpretation of the stain results is the full responsibility of the user. Any experimental result must be confirmed by a medically established diagnostic product or procedure.
Positive control: Tissue sample from breast carcinoma.
External Negative Control: Tissue sample homologous to the test sample incubated with an antibody isotype not specific for PR.

Protokoll Principles of the procedure: The demonstrations of antigens by IHC is a sequential procedure with several steps involving first the application of a specific antibody for the antigen of interest (primary antibody), then a secondary antibody which joins to the first, an enzyme complex, and the addition of a chromogenic substrate. The sample is washed between each step. Enzymatic activation of the chromogenic substrate creates a visible product where the antigen is located. The results are interpreted using a light microscope. The primary antibody can be used both in manual IHC and with automated immunostainers.

Paraffin-embedded tissue samples should be used. The antibody is also useful for immunostaining frozen tissue samples. Western blot techniques are not recommended.
Testdurchführung Antigen retrieval: HIER Citrate Buffer pH 6.5
Working dilution: (only for concentrates) 1:50 – 1:200
Incubation: 30 min, RT
Control Tissue: Breast carcinoma
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Progesterone Receptor Antikörper Target Details Progesterone Receptor Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Buffer The preparation contains saline buffer, stabilising and carriers proteins. (pH 7.4)
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store at 2-8 °C until the expiration date printed on product label. Do not use after the expiration date. If fresh solutions are required, these must be prepared immediately prior to use, and will be stable for at least one day at room temperature (20-25°C). Unused portion of antibody preparation should be discarded after one day.If the product is stored under different conditions from those stipulated in these technical indications, the new conditions must be verified by the user. The validity period of the ready to use products when opened, is the same as the expiration date indicated on the label of intact product.

Bilder

Produktdetails anti-Progesterone Receptor Antikörper Target Details Progesterone Receptor Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Progesterone Receptor (PGR) (AA 412-526) antibody (ABIN1027517) 10 × 20 × IHC of Progesterone Receptor (clone SP42) on FFPE breast carcinoma tissue. ...