anti-Maus NR0B2 Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended NR0B2 Antibody

Antigen
Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) Antikörper
  • SHP
  • SHP1
  • SHP-1
  • Shp1
  • Shp
  • NR0B2-A
  • Nr0b2
  • SHP-A
  • gb:bc058069
  • nuclear receptor subfamily 0 group B member 2
  • nuclear receptor subfamily 0, group B, member 2
  • nuclear receptor subfamily 0, group B, member 2a
  • NR0B2
  • Nr0b2
  • nr0b2a
Epitop
Ligand Binding Domain
17
11
8
7
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Reaktivität
Hamster, Human, Affe, Maus, Kaninchen
87
20
18
6
6
5
3
2
2
2
1
Wirt
Kaninchen
69
16
4
Klonalität
Polyklonal
Konjugat
Dieser NR0B2 Antikörper ist unkonjugiert
4
3
3
2
2
2
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
72
37
32
25
15
12
12
6
3
1
1
1
1
Optionen

Bilder

Produktnummer ABIN1049128
$ 484.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Clonality References Details
1 ABIN1387415 IF (p) IHC (p) WB Rabbit IgG AA 45-75 Polyclonal 0
-0.80611944 ABIN271164 IHC (p) Rabbit Polyclonal 2

General

Antigen Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) Antikörper
Epitop Ligand Binding Domain
(17), (11), (8), (7), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Hamster, Human, Affe, Maus, Kaninchen
(87), (20), (18), (6), (6), (5), (3), (2), (2), (2), (1)
Wirt Kaninchen
(69), (16), (4)
Klonalität
Polyklonal
Konjugat Dieser NR0B2 Antikörper ist unkonjugiert
(4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(72), (37), (32), (25), (15), (12), (12), (6), (3), (1), (1), (1), (1)
Hersteller

Produktdetails anti-NR0B2 Antikörper

Target Details NR0B2 Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human NR0B2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Rabbit (100%) Rat, Bovine, Bat (94%) Elephant, Panda, Horse, Pig, Opossum (88%) Dog (81%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 16 amino acid peptide from ligand-binding domain of human NR0B2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Rabbit (100%), Rat, Bovine, Bat (94%), Elephant, Panda, Horse, Pig, Opossum (88%), Dog (81%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details NR0B2

Produktdetails anti-NR0B2 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung NR0B2 / SHP (NR0B2 Antibody Abstract)
Hintergrund Name/Gene ID: NR0B2
Subfamily: NR0 Knirps-like
Family: NHR

Synonyms: NR0B2, Short heterodimer partner, SHP, Nuclear receptor SHP, Orphan nuclear receptor SHP, Small heterodimer partner, SHP1
Gen-ID 8431
Pathways Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway

Anwendungsinformationen

Produktdetails anti-NR0B2 Antikörper Target Details NR0B2 Handhabung Bilder zurück nach oben
Applikations-hinweise Approved: ICC, IF, IHC, IHC-P (4 μg/mL)
Kommentare

Target Species of Antibody: Human

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-NR0B2 Antikörper Target Details NR0B2 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-NR0B2 Antikörper Target Details NR0B2 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) (Ligand Binding Domain) antibody (ABIN1049128) Anti-NR0B2 antibody ABIN1049128 IHC staining of human liver, hepatocytes. Immunohist...
Immunohistochemistry (IHC) image for anti-Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) (Ligand Binding Domain) antibody (ABIN1049128) Anti-NR0B2 antibody IHC of human liver, hepatocytes. Immunohistochemistry of formalin...