anti-Maus NR0B2 Antikörper für Immunohistochemistry (Paraffin-embedded Sections)
Recommended NR0B2 Antibody
- Antigen
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Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) Antikörper
Synonyme für dieses Antigen anzeigen- SHP
- SHP1
- SHP-1
- Shp1
- Shp
- NR0B2-A
- Nr0b2
- SHP-A
- gb:bc058069
- nuclear receptor subfamily 0 group B member 2
- nuclear receptor subfamily 0, group B, member 2
- nuclear receptor subfamily 0, group B, member 2a
- NR0B2
- Nr0b2
- nr0b2a
- Epitop
-
Ligand Binding Domain
Alternativen - Reaktivität
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Hamster, Human, Affe, Maus, Kaninchen
Alternativen - Wirt
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Kaninchen
Alternativen - Klonalität
- Konjugat
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Dieser NR0B2 Antikörper ist unkonjugiert
Alternativen - Applikation
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Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Alternativen - Optionen
Produktnummer ABIN1049128
$ 484.00
Zzgl. Versandkosten $45.00
Zzgl. Versandkosten $45.00
Relevance Score | ABIN | Application | Konjugat | Host | Isotype | Epitope | Clonality | References | Details |
---|---|---|---|---|---|---|---|---|---|
1 | ABIN1387415 | IF (p) IHC (p) WB | Rabbit | IgG | AA 45-75 | Polyclonal | 0 | ||
-0.80611944 | ABIN271164 | IHC (p) | Rabbit | Polyclonal | 2 |
General |
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Antigen | Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2) Antikörper |
Epitop | Ligand Binding Domain Alternativen |
Reaktivität | Hamster, Human, Affe, Maus, Kaninchen Alternativen |
Wirt | Kaninchen Alternativen |
Klonalität | |
Konjugat | Dieser NR0B2 Antikörper ist unkonjugiert Alternativen |
Applikation |
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Alternativen
|
Hersteller | |
Produktdetails anti-NR0B2 AntikörperTarget Details NR0B2 Anwendungsinformationen Handhabung Bilder |
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Marke | IHC-plus™ |
Spezifität | Human NR0B2. BLAST analysis of the peptide immunogen showed no homology with other human proteins. |
Homologie | Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Rabbit (100%) Rat, Bovine, Bat (94%) Elephant, Panda, Horse, Pig, Opossum (88%) Dog (81%). |
Reinigung | Immunoaffinity purified |
Immunogen |
Synthetic 16 amino acid peptide from ligand-binding domain of human NR0B2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Rabbit (100%), Rat, Bovine, Bat (94%), Elephant, Panda, Horse, Pig, Opossum (88%), Dog (81%). Type of Immunogen: Synthetic peptide |
Plasmids, Primers & others | |
Target Details NR0B2Produktdetails anti-NR0B2 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben |
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Antigen | |
Andere Bezeichnung | NR0B2 / SHP (NR0B2 Antibody Abstract) |
Hintergrund |
Name/Gene ID: NR0B2 Subfamily: NR0 Knirps-like Family: NHR Synonyms: NR0B2, Short heterodimer partner, SHP, Nuclear receptor SHP, Orphan nuclear receptor SHP, Small heterodimer partner, SHP1 |
Gen-ID | 8431 |
Pathways | Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway |
AnwendungsinformationenProduktdetails anti-NR0B2 Antikörper Target Details NR0B2 Handhabung Bilder zurück nach oben |
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Applikations-hinweise | Approved: ICC, IF, IHC, IHC-P (4 μg/mL) |
Kommentare |
Target Species of Antibody: Human |
Testdurchführung |
The IHC-pro Immunohistochemistry Protocol Tissue Preparation Formalin fixation and embedding in paraffin wax Tissue Sectioning Make 4-µm sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-drying oven for 45 minutes at 60°C Deparaffinization Wash slides in 3 changes of xylene – 5 minutes each at room temperature. Rehydration Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature. Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature. Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature. Rinse slides in gentle running distilled water – 5 minutes at room temperature. Antigen retrieval Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes Remove from heat and let stand at room temperature in buffer - 20 minutes Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature. Immunostaining Do not allow tissues to dry at any time during the staining procedure. Apply a universal protein block – 20 minutes at room temperature. Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature. Rinse slides in 1X TBST - 1 minute at room temperature. Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature. Rinse slides 1X TBST – 1 minute at room temperature. Apply alkaline phosphatase streptavidin – 30 minutes at room temperature. Rinse slides in 1X TBST - 1 minute at room temperature. Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature. Wash slides in distilled water – 1 minute at room temperature. Dehydrate This method should only be used if the chromogen substrate is alcohol insoluble. Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature. Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature. Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature. Wash slides in 3 changes of xylene – 1 minute each at room temperature. Apply coverslip |
Beschränkungen | Nur für Forschungszwecke einsetzbar |
HandhabungProduktdetails anti-NR0B2 Antikörper Target Details NR0B2 Anwendungsinformationen Bilder zurück nach oben |
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Format | Liquid |
Konzentration | Lot specific |
Buffer | PBS, less than 0.1 % sodium azide. |
Konservierungs-mittel | Sodium azide |
Vorsichtsmaßnahmen | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Lagerung | 4 °C,-20 °C |
Informationen zur Lagerung | Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles. |
Haltbarkeit | 12 months |
BilderProduktdetails anti-NR0B2 Antikörper Target Details NR0B2 Anwendungsinformationen Handhabung zurück nach oben |
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Bilder des Herstellers |