anti-Human F2R Antikörper für Immunohistochemistry

Recommended F2R Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Coagulation Factor II (thrombin) Receptor (F2R) Antikörper
  • CF2R
  • HTR
  • PAR-1
  • PAR1
  • TR
  • Par1
  • TRGPC
  • EMK1
  • Par-1
  • Par1b
  • AI482343
  • Cf2r
  • ThrR
  • f2r-a
  • par1
  • coagulation factor II thrombin receptor
  • coagulation factor II (thrombin) receptor
  • microtubule affinity regulating kinase 2
  • coagulation factor 2 (thrombin) receptor L homeolog
  • F2R
  • F2r
  • MARK2
  • f2r.L
Epitop
N-Term, AA 61-76, Extracellular Loop
51
15
14
13
11
9
5
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reaktivität
Human
188
46
25
3
2
2
2
2
2
1
1
Wirt
Kaninchen
99
64
25
1
Klonalität
Polyklonal
Konjugat
Dieser F2R Antikörper ist unkonjugiert
6
6
5
5
5
4
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
139
95
41
36
30
26
22
13
7
2
2
1
1
1
1
1
Optionen
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Bilder

Produktnummer ABIN1742020
$ 678.57
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
9.067091 ABIN1742019 ICC IF IHC WB Rabbit N-Term, AA 61-76, Extracellular Loop Anmelden zum Anzeigen Polyclonal 1
9.067091 ABIN4343604 ELISA ICC IF IHC IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen N2-11 2
9.067091 ABIN1584987 IHC ELISA WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 2
9.067091 ABIN4899991 CyTOF FACS IHC WB Mouse IgG2b AA 27-102, AA 375-425 Anmelden zum Anzeigen 731115 0
9.067091 ABIN5683626 ELISA FACS IHC WB Mouse IgG1 AA 42-176 Anmelden zum Anzeigen 5E2E6 0
9.067091 ABIN2431755 IHC ELISA WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
9.067091 ABIN2426323 IHC ELISA Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN1876842 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN5683627 ELISA FACS IHC WB Mouse IgG1 AA 42-176 Anmelden zum Anzeigen 5E2E6 0
1 ABIN797958 IHC WB Rabbit N-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN2343289 IHC ELISA WB Goat Anmelden zum Anzeigen Polyclonal 0
1 ABIN2374650 IHC Rabbit N-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN4343610 ELISA ICC IF IHC IHC (p) FITC Mouse IgG1 Anmelden zum Anzeigen N2-11 0
1 ABIN4343611 ELISA ICC IF IHC IHC (p) WB DyLight 405 Mouse IgG1 Anmelden zum Anzeigen N2-11 0
1 ABIN4343616 ELISA ICC IF IHC IHC (p) WB DyLight 755 Mouse IgG1 Anmelden zum Anzeigen N2-11 0
1 ABIN4343626 ELISA ICC IF IHC IHC (p) WB Alexa Fluor 488 Mouse IgG1 Anmelden zum Anzeigen N2-11 0
1 ABIN4343625 ELISA ICC IF IHC IHC (p) WB Alexa Fluor 405 Mouse IgG1 Anmelden zum Anzeigen N2-11 0
1 ABIN4343628 ELISA ICC IF IHC IHC (p) WB Alexa Fluor 700 Mouse IgG1 Anmelden zum Anzeigen N2-11 0
1 ABIN4343603 ELISA ICC IF IHC IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen N2-11 0
1 ABIN4343605 ELISA ICC IF IHC IHC (p) WB Biotin Mouse IgG1 Anmelden zum Anzeigen N2-11 0

General

Antigen Coagulation Factor II (thrombin) Receptor (F2R) Antikörper
Epitop N-Term, AA 61-76, Extracellular Loop
(51), (15), (14), (13), (11), (9), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(188), (46), (25), (3), (2), (2), (2), (2), (2), (1), (1)
Wirt Kaninchen
(99), (64), (25), (1)
Klonalität
Polyklonal
Konjugat Dieser F2R Antikörper ist unkonjugiert
(6), (6), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(139), (95), (41), (36), (30), (26), (22), (13), (7), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-F2R Antikörper

Target Details F2R Anwendungsinformationen Handhabung Referenzen für anti-F2R Antikörper (ABIN1742020) Bilder
Sequenz (C)KNESGLTEYR LVSINK
Kreuzreaktivität (Details) Monkey - identical. Unlikely to recognize mouse or rat samples.
Produktmerkmale Peptide Confirmation:
Confirmed by amino acid analysis.

Buffer after reconstitution:
Phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.025 % NaN3.

Antibody concentration after reconstitution:
0.8 mg/mL

Preadsorption Control:
1 µg peptide per 1 µg antibody.

GPCR
Reinigung Affinity purified on immobilized antigen.
Immunogen Peptide corresponding to amino acid residues 61-76 of human PAR-1. Extracellular, N terminal.

Target Details F2R

Produktdetails anti-F2R Antikörper Anwendungsinformationen Handhabung Referenzen für anti-F2R Antikörper (ABIN1742020) Bilder zurück nach oben
Antigen
Andere Bezeichnung Protease-activated Receptor-1 (F2R Antibody Abstract)
Hintergrund Synonyms:
PAR-1, Thrombin receptor

Protease-activated receptor 1 (PAR-1) belongs to a family of four G protein-coupled receptors (PAR-1 - 4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their name. In this novel modality of activation, a specific protease cleaves the PAR receptor within a defined sequence in its extracellular N-terminal domain. This results in the creation of a new N-terminal tethered ligand, which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several signal transduction pathways.-3 Different PARs are activated by different proteases. Hence, PAR-1 is activated by thrombin (and is in fact also known as the thrombin receptor), as are PAR-3 and PAR-4, while PAR-2 is activated by trypsis.-3 PAR-1 can be also cleaved and activated by other proteases such as plasmin, Factor Xa, cathepsin G, and others. The intramolecular nature of PAR activation and the continuous presence of the tethered ligand that cannot diffuse away imply the existence of several mechanisms for the rapid termination of PAR signaling. Indeed, following receptor activation, there is rapid phosphorylation of the C-terminal end of the receptor, followed by receptor internalization and degradation. In addition, several proteases can cleave away the tethered ligand, thereby \\
Gen-ID 2149
UniProt P25116
Pathways Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus

Anwendungsinformationen

Produktdetails anti-F2R Antikörper Target Details F2R Handhabung Referenzen für anti-F2R Antikörper (ABIN1742020) Bilder zurück nach oben
Applikationshinweise Recommended Dilution:
WB: approx. 1:200,
IHC: approx. 1:100,
ICC: approx. 1:50,
IF: approx. 1:20
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-F2R Antikörper Target Details F2R Anwendungsinformationen Referenzen für anti-F2R Antikörper (ABIN1742020) Bilder zurück nach oben
Format Lyophilized
Rekonstitution 50 µL deionized water.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid multiple freezing and thawing.
Centrifuge all antibody preparations before use (10000 x g 5 min).
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The reconstituted solution can be stored at 4 °C for up to 2 weeks. For longer periods, small aliquots should be stored at -20 °C or below. The further dilutions should be made using a carrier protein such as BSA (1 %).

Referenzen für anti-F2R Antikörper (ABIN1742020)

Produktdetails anti-F2R Antikörper Target Details F2R Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Ossovskaya, Bunnett: "Protease-activated receptors: contribution to physiology and disease." in: Physiological reviews, Vol. 84, Issue 2, pp. 579-621, 2004

Bilder

Produktdetails anti-F2R Antikörper Target Details F2R Anwendungsinformationen Handhabung Referenzen für anti-F2R Antikörper (ABIN1742020) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Coagulation Factor II (thrombin) Receptor (F2R) (AA 61-76), (Extracellular Loop), (N-Term) antibody (ABIN1742020) Western blot analysis of human promyelocytic leukemia HL-60 (lanes 1 and 4), human T-...
Western Blotting (WB) image for anti-Coagulation Factor II (thrombin) Receptor (F2R) (AA 61-76), (Extracellular Loop), (N-Term) antibody (ABIN1742020) Western blot analysis of human colon cancer HT-29 (lanes 1 and 3) and Colo-205 (lanes...
Immunohistochemistry (IHC) image for anti-Coagulation Factor II (thrombin) Receptor (F2R) (AA 61-76), (Extracellular Loop), (N-Term) antibody (ABIN1742020) Expression of PAR-1 in normal human breast and human breast carcinoma Immunohistochem...
Immunocytochemistry (ICC) image for anti-Coagulation Factor II (thrombin) Receptor (F2R) (AA 61-76), (Extracellular Loop), (N-Term) antibody (ABIN1742020) Expression of PAR-1 in human prostate PC-3 cell line Immunocytochemical staining of h...
Flow Cytometry (FACS) image for anti-Coagulation Factor II (thrombin) Receptor (F2R) (AA 61-76), (Extracellular Loop), (N-Term) antibody (ABIN1742020) Indirect Flow cytometry analysis of live intact HL-60 (human promyelocytic leukemia) ...