anti-Ratte (Rattus) Tumor Necrosis Factor Antikörper für Immunohistochemistry (Frozen Sections)

Recommended Tumor Necrosis Factor Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Tumor Necrosis Factor (TNF) Antikörper
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Epitop
AA 100-200, Internal Region
51
44
27
27
24
21
19
16
15
11
11
10
10
8
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
1273
458
355
144
136
119
118
62
54
41
29
17
14
12
9
7
6
4
4
4
3
2
1
1
1
1
Wirt
Kaninchen
948
528
177
50
10
9
8
7
5
3
2
Klonalität
Polyklonal
Konjugat
Dieser Tumor Necrosis Factor Antikörper ist unkonjugiert
172
96
73
51
49
33
31
28
28
26
26
26
26
25
25
25
24
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Free Floating) (IHC (ff)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
916
864
621
323
288
229
223
189
143
107
97
67
29
29
26
22
20
18
14
11
9
7
6
6
6
6
5
5
3
3
3
3
2
2
2
2
2
1
1
1
1
1
Hersteller
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Produktnummer ABIN446837
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN119358 EIA Func FACS IHC (fro) IHC (p) Rat IgG1 Anmelden zum Anzeigen MP6-XT22 7
1 ABIN3201322 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 80-235 Anmelden zum Anzeigen 1
1 ABIN217631 FACS IHC (fro) ELISA Rat IgG1 Anmelden zum Anzeigen 0
1 ABIN4360942 FACS ICC IF IHC (fro) IHC (p) Mouse IgM kappa N-Term Anmelden zum Anzeigen TNF706 0
1 ABIN4360941 FACS ICC IF IHC (fro) IHC (p) Mouse IgM kappa N-Term Anmelden zum Anzeigen TNF706 0
1 ABIN4360943 FACS ICC IF IHC (fro) IHC (p) Mouse IgM kappa N-Term Anmelden zum Anzeigen TNF706 0
1 ABIN4361078 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgM kappa N-Term Anmelden zum Anzeigen TNF706 0
1 ABIN4361079 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgM kappa N-Term Anmelden zum Anzeigen TNF706 0
1 ABIN4361082 ELISA ICC IF IHC (fro) IHC (p) Biotin Mouse IgM kappa N-Term Anmelden zum Anzeigen TNF706 0
1 ABIN4361085 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgM kappa N-Term Anmelden zum Anzeigen TNF706 0
1 ABIN4361077 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgM kappa N-Term Anmelden zum Anzeigen TNF706 0
1 ABIN4361083 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgM kappa N-Term Anmelden zum Anzeigen TNF706 0
1 ABIN4361080 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgM kappa N-Term Anmelden zum Anzeigen TNF706 0
1 ABIN4361084 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgM kappa N-Term Anmelden zum Anzeigen TNF706 0
1 ABIN4361087 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgM kappa N-Term Anmelden zum Anzeigen TNF706 0
1 ABIN4361092 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgM kappa N-Term Anmelden zum Anzeigen TNF706 0
1 ABIN4361091 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgM kappa N-Term Anmelden zum Anzeigen TNF706 0
1 ABIN4361090 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgM kappa N-Term Anmelden zum Anzeigen TNF706 0
1 ABIN4360935 FACS ICC IF IHC (fro) IHC (p) Mouse IgM kappa Anmelden zum Anzeigen 4C6-H8 0
1 ABIN4360938 FACS ICC IF IHC (fro) IHC (p) Mouse IgM kappa Anmelden zum Anzeigen P-T2 0

General

Antigen Tumor Necrosis Factor (TNF) Antikörper
Epitop AA 100-200, Internal Region
(51), (44), (27), (27), (24), (21), (19), (16), (15), (11), (11), (10), (10), (8), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(1273), (458), (355), (144), (136), (119), (118), (62), (54), (41), (29), (17), (14), (12), (9), (7), (6), (4), (4), (4), (3), (2), (1), (1), (1), (1)
Wirt Kaninchen
(948), (528), (177), (50), (10), (9), (8), (7), (5), (3), (2)
Klonalität
Polyklonal
Konjugat Dieser Tumor Necrosis Factor Antikörper ist unkonjugiert
(172), (96), (73), (51), (49), (33), (31), (28), (28), (26), (26), (26), (26), (25), (25), (25), (24), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Free Floating) (IHC (ff)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(916), (864), (621), (323), (288), (229), (223), (189), (143), (107), (97), (67), (29), (29), (26), (22), (20), (18), (14), (11), (9), (7), (6), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 13 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Tumor Necrosis Factor Antikörper

Target Details Tumor Necrosis Factor Anwendungsinformationen Handhabung Referenzen für anti-Tumor Necrosis Factor Antikörper (ABIN446837) Bilder
Reinigung Immunogen affinity purified
Immunogen A synthetic peptide made to an internal portion of the human TNF alpha protein (between residues 100-200) [UniProt P01375]

Target Details Tumor Necrosis Factor

Produktdetails anti-Tumor Necrosis Factor Antikörper Anwendungsinformationen Handhabung Referenzen für anti-Tumor Necrosis Factor Antikörper (ABIN446837) Bilder zurück nach oben
Antigen
Andere Bezeichnung TNF-alpha (TNF Antibody Abstract)
Hintergrund Gene Symbol: TNF
Gen-ID 7124
UniProt P01375
Pathways NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Anwendungsinformationen

Produktdetails anti-Tumor Necrosis Factor Antikörper Target Details Tumor Necrosis Factor Handhabung Referenzen für anti-Tumor Necrosis Factor Antikörper (ABIN446837) Bilder zurück nach oben
Applikationshinweise Western Blot 1:250 - 1:500, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:200 - 1:300, Immunohistochemistry-Paraffin 1:200, Immunohistochemistry-Frozen, Immunohistochemistry Free-FloatingWestern Blot data from customer review and citation (PMID: 24223475). Use in Immunohistochemistry-free floating reported in scientific literature (PMID 26219646) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 26725948)
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Immunohistochemistry Protocol specific for TNF alpha antibody Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
1. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.Immunocytochemistry Protocol specific for TNF alpha antibody Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Tumor Necrosis Factor Antikörper Target Details Tumor Necrosis Factor Anwendungsinformationen Referenzen für anti-Tumor Necrosis Factor Antikörper (ABIN446837) Bilder zurück nach oben
Format Liquid
Konzentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Referenzen für anti-Tumor Necrosis Factor Antikörper (ABIN446837)

Produktdetails anti-Tumor Necrosis Factor Antikörper Target Details Tumor Necrosis Factor Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Tucker, Riggin, Connizzo, Mauck, Steinberg, Kuntz, Soslowsky, Bernstein: "Effect of overuse-induced tendinopathy on tendon healing in a rat supraspinatus repair model." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 34, Issue 1, pp. 161-6, 2016 (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Vieira Ramos, Pinheiro, Messa, Delfino, Marqueti, Salvini, Durigan: "Cryotherapy Reduces Inflammatory Response Without Altering Muscle Regeneration Process and Extracellular Matrix Remodeling of Rat Muscle." in: Scientific reports, Vol. 6, pp. 18525, 2016 (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunohistochemistry (Frozen Sections)

Xu, Romero, Miller, Kadam, Mial, Plazyo, Garcia-Flores, Hassan, Xu, Tarca, Drewlo, Gomez-Lopez: "An M1-like Macrophage Polarization in Decidual Tissue during Spontaneous Preterm Labor That Is Attenuated by Rosiglitazone Treatment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 196, Issue 6, pp. 2476-91, 2016 (Probematerial (Species): Human). Weitere Details: Flow Cytometry,Immunohistochemistry (Frozen Sections)

Szylberg, Janiczek, Popiel, Marszałek: "Expression of COX-2, IL-1β, TNF-α and IL-4 in epithelium of serrated adenoma, adenoma and hyperplastic polyp." in: Archives of medical science : AMS, Vol. 12, Issue 1, pp. 172-8, 2016 (Probematerial (Species): Human). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Veruva, Lanman, Isaza, Freeman, Kurtz, Steinbeck: "Periprosthetic UHMWPE Wear Debris Induces Inflammation, Vascularization, and Innervation After Total Disc Replacement in the Lumbar Spine." in: Clinical orthopaedics and related research, 2016 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry

Acosta, Tajiri, Hoover, Kaneko, Borlongan: "Intravenous Bone Marrow Stem Cell Grafts Preferentially Migrate to Spleen and Abrogate Chronic Inflammation in Stroke." in: Stroke, Vol. 46, Issue 9, pp. 2616-27, 2015 (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunohistochemistry (Free Floating)

Piao, Yuan, Wang, Sun, Liang, Ma: "α-catenin acts as a tumour suppressor in E-cadherin-negative basal-like breast cancer by inhibiting NF-κB signalling." in: Nature cell biology, Vol. 16, Issue 3, pp. 245-54, 2014 (Probematerial (Species): Human). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Thomas, Sarver, Yannascoli, Tucker, Kelly, Ahima, Barbe, Soslowsky: "Effect of isolated hyperglycemia on native mechanical and biologic shoulder joint properties in a rat model." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 32, Issue 11, pp. 1464-70, 2014 (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Castoldi, di Gioia, Bombardi, Maestroni, Carletti, Steckelings, Dahlöf, Unger, Zerbini, Stella: "PREVENTION OF DIABETIC NEPHROPATHY BY COMPOUND 21, SELECTIVE AGONIST OF THE ANGIOTENSIN TYPE 2 RECEPTORS, IN ZUCKER DIABETIC FATTY RATS." in: American journal of physiology. Renal physiology, pp. ajprenal.00247.2014, 2014

Chen, Shi, Zhang, Lei, Long, Su, Pei, Huang: "Scutellarin attenuates hypertension-induced expression of brain Toll-like receptor 4/nuclear factor kappa B." in: Mediators of inflammation, Vol. 2013, pp. 432623, 2013 (Probematerial (Species): Rat (Rattus)). Weitere Details: Western Blotting

Kwon, Jung, Hwang, Choi: "Evaluation of Bronchiolar and Alveolar Cell Injuries Induced by Short- and Long-term Exposure to Sidestream Smoke." in: Korean journal of pathology, Vol. 46, Issue 2, pp. 151-61, 2012

Mohamed, Coucha, Elshaer, Artham, Lemtalsi, El-Remessy: "Inducible overexpression of endothelial proNGF as a mouse model to study microvascular dysfunction." in: Biochimica et biophysica acta, Vol. 1864, Issue 3, pp. 746-757, 1970 (Probematerial (Species): Mouse (Murine)).

Özdemir, Altun, Arslan et al.: "Imidacloprid exposure cause the histopathological changes, activation of TNF-α, iNOS, 8-OHdG biomarkers, and alteration of caspase 3, iNOS, CYP1A, MT1 gene expression levels in common carp (Cyprinus ..." in: Toxicology reports, Vol. 5, pp. 125-133, 1970 Von den Autoren verwendete Methode: Immunofluorescence (fixed cells) (IF/ICC) (Probematerial (Species): Fish).

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Produktdetails anti-Tumor Necrosis Factor Antikörper Target Details Tumor Necrosis Factor Anwendungsinformationen Handhabung Referenzen für anti-Tumor Necrosis Factor Antikörper (ABIN446837) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tumor Necrosis Factor (TNF) (AA 100-200), (Internal Region) antibody (ABIN446837) Immunohistochemistry-Paraffin: TNF-alpha Antibody [NBP1-19532] - Staining of TNF alph...
Immunofluorescence (IF) image for anti-Tumor Necrosis Factor (TNF) (AA 100-200), (Internal Region) antibody (ABIN446837) Immunocytochemistry/Immunofluorescence: TNF-alpha Antibody [NBP1-19532] - TNF alpha a...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Tumor Necrosis Factor (TNF) (AA 100-200), (Internal Region) antibody (ABIN446837) Immunohistochemistry-Paraffin: TNF-alpha Antibody - IHC analysis of a formalin fixed...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Tumor Necrosis Factor (TNF) (AA 100-200), (Internal Region) antibody (ABIN446837) Immunohistochemistry-Paraffin: TNF-alpha Antibody - IHC analysis of a formalin fixed...