anti-Human p65 Antikörper für Immunofluorescence

Recommended p65 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Nuclear Factor-KB P65 (NFkBP65) Antikörper
  • C-Rel
  • NFKB3
  • p65
  • NFkB
  • c-rel
  • zgc:100833
  • REL proto-oncogene, NF-kB subunit
  • RELA proto-oncogene, NF-kB subunit
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel avian reticuloendotheliosis viral oncogene homolog
  • REL
  • RELA
  • Rela
  • Rel
  • rel
Epitop
Center
131
85
69
63
49
45
41
39
38
37
26
25
25
18
17
16
15
15
13
13
13
12
11
10
8
8
7
6
6
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
1151
667
520
72
56
55
30
25
25
22
13
8
8
6
4
2
1
1
Wirt
Kaninchen
1008
132
17
10
1
Klonalität
Polyklonal
Konjugat
Dieser p65 Antikörper ist unkonjugiert
30
29
23
20
18
18
17
13
12
12
12
8
8
8
8
8
8
8
8
4
4
3
3
2
2
2
2
2
2
1
Applikation
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
853
445
331
257
205
162
105
92
87
28
19
18
15
12
11
6
6
6
5
5
4
2
2
2
1
Optionen
Hersteller
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Bilder

Produktnummer ABIN2855287
$ 358.60
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
11.758385 ABIN257452 ICC IF IHC (p) IHC WB Rabbit pSer276 Anmelden zum Anzeigen Polyclonal 2
11.758385 ABIN2705043 IC IF IP IHC WB Rabbit C-Term, pSer435 Anmelden zum Anzeigen Polyclonal 0
11.758385 ABIN2706680 IC IF IHC WB Rabbit pSer311 Anmelden zum Anzeigen Polyclonal 0
11.758385 ABIN2706682 IC IF IHC WB Rabbit Center Anmelden zum Anzeigen Polyclonal 0
11.758385 ABIN2706683 ChIP IC IF IHC WB Rabbit acLys310 Anmelden zum Anzeigen Polyclonal 0
11.758385 ABIN1535550 IF IHC ELISA WB Rabbit IgG AA 275-324 Anmelden zum Anzeigen Polyclonal 2
11.758385 ABIN4349861 ELISA ICC IF IHC IHC (p) WB Mouse IgG2a kappa C-Term Anmelden zum Anzeigen 27F9-G4 2
11.758385 ABIN4349848 ICC IF WB Rabbit Anmelden zum Anzeigen Polyclonal 1
11.758385 ABIN1535530 IF IHC ELISA WB Rabbit IgG AA 275-324, acLys310 Anmelden zum Anzeigen Polyclonal 0
11.758385 ABIN256430 ICC IF IHC (p) IP IHC WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal 1
11.758385 ABIN4349864 ICC IF IHC IHC (p) WB Rabbit AA 300-400, Internal Region Anmelden zum Anzeigen Polyclonal 3
11.758385 ABIN2852659 IC IF IP IHC WB Mouse Anmelden zum Anzeigen 0
11.758385 ABIN1870574 IF IHC WB Rabbit IgG pThr254 Anmelden zum Anzeigen Polyclonal 0
11.758385 ABIN1870578 IF IHC WB Rabbit IgG pSer536 Anmelden zum Anzeigen Polyclonal 0
11.758385 ABIN1870585 IF WB Rabbit IgG pSer468 Anmelden zum Anzeigen Polyclonal 0
11.758385 ABIN1870587 IF IHC WB Rabbit IgG pThr435 Anmelden zum Anzeigen Polyclonal 0
11.758385 ABIN1870581 IF IHC WB Rabbit IgG pSer529 Anmelden zum Anzeigen Polyclonal 0
11.758385 ABIN1870583 IF IHC WB Rabbit IgG pSer311 Anmelden zum Anzeigen Polyclonal 0
11.758385 ABIN1537682 IF WB Rabbit Ig Fraction AA 517-539, C-Term Anmelden zum Anzeigen Polyclonal 0
11.758385 ABIN4349846 ICC IF IHC IHC (p) WB Rabbit pSer529 Anmelden zum Anzeigen Polyclonal 0

General

Antigen Nuclear Factor-KB P65 (NFkBP65) Antikörper
Epitop Center
(131), (85), (69), (63), (49), (45), (41), (39), (38), (37), (26), (25), (25), (18), (17), (16), (15), (15), (13), (13), (13), (12), (11), (10), (8), (8), (7), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus
(1151), (667), (520), (72), (56), (55), (30), (25), (25), (22), (13), (8), (8), (6), (4), (2), (1), (1)
Wirt Kaninchen
(1008), (132), (17), (10), (1)
Klonalität
Polyklonal
Konjugat Dieser p65 Antikörper ist unkonjugiert
(30), (29), (23), (20), (18), (18), (17), (13), (12), (12), (12), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Applikation Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(853), (445), (331), (257), (205), (162), (105), (92), (87), (28), (19), (18), (15), (12), (11), (6), (6), (6), (5), (5), (4), (2), (2), (2), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-p65 Antikörper

Target Details p65 Anwendungsinformationen Handhabung Bilder
Kreuzreaktivität Rind (Kuh), Schwein, Ratte (Rattus)
Kreuzreaktivität (Details) Pig (98 %), Rat (99 %), Bovine (98 %)
Produktmerkmale Rabbit Polyclonal antibody to NFkB p65 (v-rel reticuloendotheliosis viral oncogene homolog A (avian))
NFkB p65 antibody
Reinigung Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human NFkB p65. The exact sequence is proprietary.
Isotyp IgG

Target Details p65

Produktdetails anti-p65 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung NFkB p65 (NFkBP65 Antibody Abstract)
Hintergrund NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).[supplied by OMIM]

Cellular Localization: Nucleus , Cytoplasm
Molekulargewicht 60 kDa
Gen-ID 5970
Pathways NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-p65 Antikörper Target Details p65 Handhabung Bilder zurück nach oben
Applikationshinweise Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:10000*
Kommentare

Positive Control: A431 , HepG2 , human NFkB p65-transfected 293T

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1.03 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
Konservierungsmittel ProClin
Vorsichtsmaßnahmen This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Bilder

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Nuclear Factor-KB P65 (NFkBP65) (Center) antibody (ABIN2855287) ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde...
Western Blotting (WB) image for anti-Nuclear Factor-KB P65 (NFkBP65) (Center) antibody (ABIN2855287) WB Image NFkB p65 antibody detects NFkB p65 protein by western blot analysis. Non-tra...
Immunohistochemistry (IHC) image for anti-Nuclear Factor-KB P65 (NFkBP65) (Center) antibody (ABIN2855287) IHC-P Image NFkB p65 antibody detects NFkB p65 protein at cytosol and nucleus on huma...
Western Blotting (WB) image for anti-Nuclear Factor-KB P65 (NFkBP65) (Center) antibody (ABIN2855287) WB Image Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the...
Immunohistochemistry (IHC) image for anti-Nuclear Factor-KB P65 (NFkBP65) (Center) antibody (ABIN2855287) IHC-P Image NFkB p65 antibody detects NFkB p65 protein at cytosol on mouse heart by i...
Western Blotting (WB) image for anti-Nuclear Factor-KB P65 (NFkBP65) (Center) antibody (ABIN2855287) WB Image Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) wer...