Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

100bp DNA Ladder

100-1500 bp Fluorometric
Produktnummer ABIN1540468
  • Nachweismethode
    Fluorometric
    Detektionsbereich
    100-1500 bp
    Untere Nachweisgrenze
    100 bp
    Applikation
    Agarose Gel Electrophoresis (AGE)
    Verwendungszweck
    100bp DNA Ladder (Quantitative) is ideal for determining the size of double-stranded DNA from 100 to 1500 base pairs.
    Produktmerkmale
    The ladder consists of 11 linear double-stranded DNA fragments, chromatography-purified. The intensity of the 500bp has been increased to serve as a reference for easy identification. All fragments are precisely quantified and mixed during the production. For 5 µL loading, all fragments except 500bp are at 40ng. The 500bp fragment is at 100ng. This ladder is pre-mixed with loading dye and is ready to use.
    11 DNA Fragments (bp): 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, and 1500
    500bp Band: 100ng/5 µL
    Other Bands: 40ng/5 µL
    Range: 100bp-1.5kb
    Bands: 11
  • Applikationshinweise
    Recommended Loading: 5 µL/Lane.
    Add 5 µL ladder directly to each well. You may need more or less than 5 µL of ladder, depending on well size and level of intensity needed to visualize the bands.
    Recommended Loading: 5 µL/Lane, 8cm, 1.7% Agarose Gel, 0.5×TBE, 5V/cm, 1h.
    Kommentare

    Do not heat before loading.
    For quantification, adjust the concentration of the sample to equalize.
    Visualize DNA by staining with ethidium bromide or with SYBR Green I.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Lagerung
    -20 °C
    Informationen zur Lagerung
    1 year at room temperature, for long term storage, please keep at -20°C.
    Haltbarkeit
    12 months
Sie sind hier:
Kundenservice