anti-Maus BAD Antikörper für Western Blotting

Recommended BAD Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
BCL2-Associated Agonist of Cell Death (BAD) Antikörper
  • bad
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
  • BAD
  • BBC2
  • BCL2L8
  • AI325008
  • Bbc2
  • BCL2-associated agonist of cell death b
  • BCL2 associated agonist of cell death
  • BCL2-associated agonist of cell death
  • badb
  • BAD
  • Bad
Epitop
AA 140-168
60
59
47
47
47
47
44
39
34
22
19
19
19
19
19
19
18
18
18
18
18
18
18
18
18
18
18
18
17
17
15
15
14
11
11
11
10
9
9
8
7
7
6
6
5
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
793
484
317
21
19
8
5
4
3
2
2
1
1
1
1
1
1
Wirt
Kaninchen
1004
46
2
1
Klonalität
Polyklonal
Konjugat
Dieser BAD Antikörper ist unkonjugiert
67
66
66
60
60
60
6
6
6
6
6
6
6
6
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
555
553
405
352
140
78
53
42
14
13
12
10
4
3
2
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN674709
$ 306.90
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
3.0650759 ABIN2779324 WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 1
3.0650759 ABIN2779311 WB Rabbit Middle Region Anmelden zum Anzeigen Polyclonal 2
3.0650759 ABIN2705560 IHC WB Rabbit pSer136 Anmelden zum Anzeigen Polyclonal 0
3.0650759 ABIN2704518 IHC WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0
3.0650759 ABIN1531523 IHC ELISA WB Rabbit IgG AA 61-110, pSer91 Anmelden zum Anzeigen Polyclonal 1
3.0650759 ABIN1531524 IF IHC ELISA WB Rabbit IgG AA 100-149, pSer134 Anmelden zum Anzeigen Polyclonal 1
3.0650759 ABIN1532540 IHC ELISA WB Rabbit IgG AA 100-149 Anmelden zum Anzeigen Polyclonal 1
3.0650759 ABIN1531780 IHC ELISA WB Rabbit IgG AA 119-168, pSer118 Anmelden zum Anzeigen Polyclonal 0
3.0650759 ABIN1531781 IHC ELISA WB Rabbit IgG AA 78-127, pSer75 Anmelden zum Anzeigen Polyclonal 0
3.0650759 ABIN1532810 IHC ELISA WB Rabbit IgG AA 119-168 Anmelden zum Anzeigen Polyclonal 0
3.0650759 ABIN1532812 IHC ELISA WB Rabbit IgG AA 102-151 Anmelden zum Anzeigen Polyclonal 0
3.0650759 ABIN1532811 IHC ELISA WB Rabbit IgG AA 78-127 Anmelden zum Anzeigen Polyclonal 0
3.0650759 ABIN1531782 IHC ELISA WB Rabbit IgG AA 102-151, pSer99 Anmelden zum Anzeigen Polyclonal 0
3.0650759 ABIN388118 FACS IHC (p) WB Rabbit Ig Fraction AA 92-127, BH3 Domain Anmelden zum Anzeigen Polyclonal 3
3.0650759 ABIN1536078 IHC ELISA WB Rabbit IgG AA 21-70, Cleaved-Asp71 Anmelden zum Anzeigen Polyclonal 0
3.0650759 ABIN2862599 IHC ELISA WB Rabbit IgG pSer136 Anmelden zum Anzeigen Polyclonal 0
3.0650759 ABIN356355 EIA WB Mouse IgG1 pSer155, pSer118 Anmelden zum Anzeigen 27AT937 0
3.0650759 ABIN356354 EIA WB Mouse IgG1 pSer155, pSer118 Anmelden zum Anzeigen 27AT381 0
3.0650759 ABIN4282857 IHC IHC (p) WB Rabbit Anmelden zum Anzeigen Polyclonal 1
3.0650759 ABIN1870003 WB Rabbit IgG pSer75 Anmelden zum Anzeigen Polyclonal 0

General

Antigen BCL2-Associated Agonist of Cell Death (BAD) Antikörper
Epitop AA 140-168
(60), (59), (47), (47), (47), (47), (44), (39), (34), (22), (19), (19), (19), (19), (19), (19), (18), (18), (18), (18), (18), (18), (18), (18), (18), (18), (18), (18), (17), (17), (15), (15), (14), (11), (11), (11), (10), (9), (9), (8), (7), (7), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus
(793), (484), (317), (21), (19), (8), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Wirt Kaninchen
(1004), (46), (2), (1)
Klonalität
Polyklonal
Konjugat Dieser BAD Antikörper ist unkonjugiert
(67), (66), (66), (60), (60), (60), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Applikation Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(555), (553), (405), (352), (140), (78), (53), (42), (14), (13), (12), (10), (4), (3), (2), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-BAD Antikörper

Target Details BAD Anwendungsinformationen Handhabung Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human Bad
Isotyp IgG
Plasmids, Primers & others

Target Details BAD

Produktdetails anti-BAD Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Bad (BAD Antibody Abstract)
Hintergrund

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Subcellular location: Cytoplasm, Mitochondrion

Synonyms: BBC2, BCL2L8, Bcl2-associated agonist of cell death, BAD, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl2-L-8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, BBC6

Gen-ID 572
UniProt Q92934
Pathways MAPK Signalweg, PI3K-Akt Signalweg, RTK Signalweg, Apoptose, Fc-epsilon Rezeptor Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events

Anwendungsinformationen

Produktdetails anti-BAD Antikörper Target Details BAD Handhabung Bilder zurück nach oben
Applikations-hinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-BAD Antikörper Target Details BAD Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

Bilder

Produktdetails anti-BAD Antikörper Target Details BAD Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Lane 1:Mouse kidney lysate Lane 2: Mouse brain lysate probed with Rabbit Anti-Bad Pol...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-Bad Polyclonal A...
Western Blotting (WB) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Image provided by the Independent Validation Program (badge number 29802). Lane 1: MC...
'Independent Validation' Siegel
Chargennummer 090915
Validierte Anwendung Western Blotting
Positivkontrolle MCF7 cells
Negativkontrolle C6/36 cells (non-reactive species)
Bewertung A strong band was observed in the positive control sample at the correct molecular weight. No bands were observed in the negative control sample.
Primärantikörper
Sekundärantikörper
Kontrollen
  • Positive control: MCF7 cells
  • Negative control: C6/36 cells
Protokoll
  • 1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
  • 2. 15 μl of heated extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • 5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
  • 6. The membrane was rinsed with TBST once.
  • 7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 16 hours at 4°C.
  • 8. The membrane was rinsed in TBST thrice for 5 minutes each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
  • 11. The membrane was rinsed in TBS twice for 30 seconds each.
  • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 600 seconds.
  • 13. The membrane was rinsed three times TBST.
  • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
  • 15. The membrane was washed in TBST 2 times for 10 minutes each.
  • 16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.
Anmerkungen - No experimental challenges noted.
Validierungsbilder
Western Blotting validation image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Figure 1: Western Blot for BAD. Arrowhead indicates the expected molecular weight of ...