anti-Human BAD Antikörper für Enzyme Immunoassay

Recommended BAD Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
BCL2-Associated Agonist of Cell Death (BAD) Antikörper
  • bad
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
  • BAD
  • BBC2
  • BCL2L8
  • AI325008
  • Bbc2
  • BCL2-associated agonist of cell death b
  • BCL2 associated agonist of cell death
  • BCL2-associated agonist of cell death
  • badb
  • BAD
  • Bad
Epitop
pSer99
58
58
47
46
46
45
44
39
32
22
19
19
19
19
19
19
18
18
18
18
18
18
18
18
18
18
18
18
17
17
15
14
14
11
11
11
10
9
9
8
7
7
6
5
5
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
778
469
301
19
19
8
5
4
3
2
2
1
1
1
1
1
1
Wirt
Kaninchen
988
46
2
1
Klonalität
Polyklonal
Konjugat
Dieser BAD Antikörper ist unkonjugiert
67
66
66
60
60
60
6
6
6
6
6
6
6
6
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
543
538
405
347
124
79
53
37
14
12
11
10
4
3
2
1
1
1
Hersteller
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Bilder

Produktnummer ABIN358087
$ 390.50
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN5552120 EIA IHC (p) WB Rabbit Ig Fraction pSer118 Anmelden zum Anzeigen Polyclonal 0
1 ABIN5552121 EIA IHC (p) WB Rabbit Ig Fraction pSer134 Anmelden zum Anzeigen Polyclonal 0
1 ABIN5552122 EIA IHC (p) WB Rabbit Ig Fraction pSer75 Anmelden zum Anzeigen Polyclonal 0
1 ABIN356355 EIA WB Mouse IgG1 pSer155, pSer118 Anmelden zum Anzeigen 27AT937 0
1 ABIN356354 EIA WB Mouse IgG1 pSer155, pSer118 Anmelden zum Anzeigen 27AT381 0
1 ABIN5552012 EIA IHC (p) Rabbit Ig Fraction AA 100-130, BH3 Domain Anmelden zum Anzeigen Polyclonal 0
1 ABIN5552009 EIA WB Rabbit Middle Region Anmelden zum Anzeigen Polyclonal 0
1 ABIN265323 EIA IHC (p) WB Rabbit Anmelden zum Anzeigen Polyclonal 0
1 ABIN5554581 EIA IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN317970 EIA IHC (p) WB Rabbit pSer118, pSer155 Anmelden zum Anzeigen Polyclonal 0
1 ABIN317969 EIA IHC (p) WB Rabbit pSer136, pSer99 Anmelden zum Anzeigen Polyclonal 0
1 ABIN2716580 EIA Rabbit IgG Anmelden zum Anzeigen Polyclonal 0

General

Antigen BCL2-Associated Agonist of Cell Death (BAD) Antikörper
Epitop pSer99
(58), (58), (47), (46), (46), (45), (44), (39), (32), (22), (19), (19), (19), (19), (19), (19), (18), (18), (18), (18), (18), (18), (18), (18), (18), (18), (18), (18), (17), (17), (15), (14), (14), (11), (11), (11), (10), (9), (9), (8), (7), (7), (6), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(778), (469), (301), (19), (19), (8), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Wirt Kaninchen
(988), (46), (2), (1)
Klonalität
Polyklonal
Konjugat Dieser BAD Antikörper ist unkonjugiert
(67), (66), (66), (60), (60), (60), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Applikation Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(543), (538), (405), (347), (124), (79), (53), (37), (14), (12), (11), (10), (4), (3), (2), (1), (1), (1)
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-BAD Antikörper

Target Details BAD Anwendungsinformationen Handhabung Referenzen für anti-BAD Antikörper (ABIN358087) Bilder
Spezifität This antibody detects Bcl-2-like 8/BAD pSer99. Predicted to cross react with Mouse (100 % Antigen Homology).
Reinigung Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S99 of human Bad.
Isotyp Ig Fraction
Plasmids, Primers & others

Target Details BAD

Produktdetails anti-BAD Antikörper Anwendungsinformationen Handhabung Referenzen für anti-BAD Antikörper (ABIN358087) Bilder zurück nach oben
Antigen
Hintergrund Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.Synonyms: BAD, BBC6, BCL2L8, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-XL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-L-8
Molekulargewicht 18392 Da
Gen-ID 572, 9606
UniProt Q92934
Pathways MAPK Signalweg, PI3K-Akt Signalweg, RTK Signalweg, Apoptose, Fc-epsilon Rezeptor Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events

Anwendungsinformationen

Produktdetails anti-BAD Antikörper Target Details BAD Handhabung Referenzen für anti-BAD Antikörper (ABIN358087) Bilder zurück nach oben
Applikations-hinweise ELISA: 1/1,000. Western Blot: 1/100-1/500. Dot Blot: 1/500. Immunohistochemistry: 1/50-1/100.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-BAD Antikörper Target Details BAD Anwendungsinformationen Referenzen für anti-BAD Antikörper (ABIN358087) Bilder zurück nach oben
Format Liquid
Konzentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Referenzen für anti-BAD Antikörper (ABIN358087)

Produktdetails anti-BAD Antikörper Target Details BAD Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Hurbin, Coll, Dubrez-Daloz, Mari, Auberger, Brambilla, Favrot: "Cooperation of amphiregulin and insulin-like growth factor-1 inhibits Bax- and Bad-mediated apoptosis via a protein kinase C-dependent pathway in non-small cell lung cancer cells." in: The Journal of biological chemistry, Vol. 280, Issue 20, pp. 19757-67, 2005

Antignani, Youle: "A chimeric protein induces tumor cell apoptosis by delivering the human Bcl-2 family BH3-only protein Bad." in: Biochemistry, Vol. 44, Issue 10, pp. 4074-82, 2005

Ying, Seiffert, Häcker, Fischer: "Broad degradation of proapoptotic proteins with the conserved Bcl-2 homology domain 3 during infection with Chlamydia trachomatis." in: Infection and immunity, Vol. 73, Issue 3, pp. 1399-403, 2005

Seo, Chen, Ivanovska, Ranger, Hong, Dawson, Korsmeyer, Bellows, Fannjiang, Hardwick: "BAD is a pro-survival factor prior to activation of its pro-apoptotic function." in: The Journal of biological chemistry, Vol. 279, Issue 40, pp. 42240-9, 2004

Lee, Soung, Kim, Nam, Kim, Cho, Lee, Kim, Park, Kim, Lee, Yoo, Lee: "Inactivating mutations of proapoptotic Bad gene in human colon cancers." in: Carcinogenesis, Vol. 25, Issue 8, pp. 1371-6, 2004

Bilder

Produktdetails anti-BAD Antikörper Target Details BAD Anwendungsinformationen Handhabung Referenzen für anti-BAD Antikörper (ABIN358087) zurück nach oben
Bilder des Herstellers
 image for anti-BCL2-Associated Agonist of Cell Death (BAD) (pSer99) antibody (ABIN358087) anti-BCL2-Associated Agonist of Cell Death (BAD) (pSer99) antibody
 image for anti-BCL2-Associated Agonist of Cell Death (BAD) (pSer99) antibody (ABIN358087) anti-BCL2-Associated Agonist of Cell Death (BAD) (pSer99) antibody (Image 2)