HAGH HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
-
- Target Alle HAGH Produkte
- HAGH (Hydroxyacylglutathione Hydrolase (HAGH))
- Proteinspezies
- Human
- Lysatspezies
- Human Cells
- Applikation
- Immunoprecipitation (IP), Western Blotting (WB)
- Verwendungszweck
- Transient Overexpression Lysate
- Spezifität
- Non-denatured
- Produktmerkmale
-
HAGH HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-HAGH full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
-
- Applikationshinweise
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Kommentare
-
Product Quality tested by: Transient overexpression cell lysate was tested with Anti-HAGH antibody by Western Blots.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- 2 mg/mL
- Buffer
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handhabung
- Aliquot to avoid repeated freezing and thawing.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
-
- Target
- HAGH (Hydroxyacylglutathione Hydrolase (HAGH))
- Andere Bezeichnung
- HAGH (HAGH Produkte)
- Synonyme
- glo-2 Lysate, glo2 Lysate, glx2 Lysate, glxii Lysate, hagh1 Lysate, DDBDRAFT_0186675 Lysate, DDBDRAFT_0230988 Lysate, DDB_0186675 Lysate, DDB_0230988 Lysate, DDBDRAFT_0183924 Lysate, DDBDRAFT_0230991 Lysate, DDB_0183924 Lysate, DDB_0230991 Lysate, BC019817 Lysate, Glo-2 Lysate, Glo2 Lysate, Rsp29 Lysate, RSP29 Lysate, fa66a03 Lysate, wu:fa66a03 Lysate, zgc:73161 Lysate, GLO2 Lysate, GLX2 Lysate, GLXII Lysate, HAGH1 Lysate, hydroxyacylglutathione hydrolase L homeolog Lysate, hydroxyacylglutathione hydrolase Lysate, beta-lactamase domain-containing protein Lysate, hydroxyacyl glutathione hydrolase Lysate, hagh.L Lysate, CND02510 Lysate, gloB1 Lysate, gloB2 Lysate, Fbal_1466 Lysate, Hagh Lysate, hagh Lysate, HAGH Lysate
- Hintergrund
-
Full Gene Name: hydroxyacylglutathione hydrolase
Synonyms: GLO2,GLX2,GLXII,HAGH1 - Gen-ID
- 3029
- NCBI Accession
- NP_005317, NM_005326
-