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HAGH HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

HAGH IP, WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
Produktnummer ABIN1339747
  • Target Alle HAGH Produkte
    HAGH (Hydroxyacylglutathione Hydrolase (HAGH))
    Proteinspezies
    Human
    Lysatspezies
    Human Cells
    Applikation
    Immunoprecipitation (IP), Western Blotting (WB)
    Verwendungszweck
    Transient Overexpression Lysate
    Spezifität
    Non-denatured
    Produktmerkmale
    HAGH HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
    Used Plasmid: pCMV-HAGH full length
    Lysed Cells
    HEK 293T Cells
    Lysate Type
    Overexpression Lysate
    Lysate Fraction
    Whole Cell Lysate
  • Applikationshinweise
    Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
    Kommentare

    Product Quality tested by: Transient overexpression cell lysate was tested with Anti-HAGH antibody by Western Blots.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    2 mg/mL
    Buffer
    Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
    Handhabung
    Aliquot to avoid repeated freezing and thawing.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
  • Target
    HAGH (Hydroxyacylglutathione Hydrolase (HAGH))
    Andere Bezeichnung
    HAGH (HAGH Produkte)
    Synonyme
    glo-2 Lysate, glo2 Lysate, glx2 Lysate, glxii Lysate, hagh1 Lysate, DDBDRAFT_0186675 Lysate, DDBDRAFT_0230988 Lysate, DDB_0186675 Lysate, DDB_0230988 Lysate, DDBDRAFT_0183924 Lysate, DDBDRAFT_0230991 Lysate, DDB_0183924 Lysate, DDB_0230991 Lysate, BC019817 Lysate, Glo-2 Lysate, Glo2 Lysate, Rsp29 Lysate, RSP29 Lysate, fa66a03 Lysate, wu:fa66a03 Lysate, zgc:73161 Lysate, GLO2 Lysate, GLX2 Lysate, GLXII Lysate, HAGH1 Lysate, hydroxyacylglutathione hydrolase L homeolog Lysate, hydroxyacylglutathione hydrolase Lysate, beta-lactamase domain-containing protein Lysate, hydroxyacyl glutathione hydrolase Lysate, hagh.L Lysate, CND02510 Lysate, gloB1 Lysate, gloB2 Lysate, Fbal_1466 Lysate, Hagh Lysate, hagh Lysate, HAGH Lysate
    Hintergrund
    Full Gene Name: hydroxyacylglutathione hydrolase
    Synonyms: GLO2,GLX2,GLXII,HAGH1
    Gen-ID
    3029
    NCBI Accession
    NP_005317, NM_005326
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