HUVEC(human umbilical vein) whole cell lysate (non-denatured)

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Proteinspezies
Human
Lysatspezies
Human Cells
Applikation
Immunoprecipitation (IP), Western Blotting (WB)
Optionen
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Verwendungszweck Whole cell lysate
Spezifität Non-denatured
Produktmerkmale HUVEC(human umbilical vein) whole cell lysate (non-denatured)
Used Tissue: Human Umbilical Vein
Lysate Fraction Whole Cell Lysate
Lysate Type Cell Lysate
Lysed Cells HUVEC Cells
Hintergrund Full Gene Name:
Synonyms:
Applikations-hinweise Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Kommentare

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
Handhabung Aliquot to avoid repeated freezing and thawing.
Lagerung -80 °C
Informationen zur Lagerung Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
Bilder des Herstellers
 image for HUVEC(human umbilical vein) whole cell lysate (non-denatured) (ABIN1339669) HUVEC(human umbilical vein) whole cell lysate (non-denatured)
Produkt verwendet in: Acevedo, Caro-Oleas, Álvarez-Márquez, Sobrino, Lage-Gallé, Aguilera, Núñez-Roldán: "Antibodies against heterogeneous nuclear ribonucleoprotein K in patients with cardiac allograft vasculopathy." in: The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation, Vol. 30, Issue 9, pp. 1051-9, 2011 (PubMed).