hES-TW1(human embryonic stem cell) whole cell lysate (non-denatured)

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Proteinspezies
Human
Lysatspezies
Human
Applikation
Immunoprecipitation (IP), Western Blotting (WB)
Optionen
Hersteller
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Verwendungszweck Whole cell lysate
Spezifität Non-denatured
Produktmerkmale hES-TW1(human embryonic stem cell) whole cell lysate (non-denatured)
Used Tissue: Embryonic
Lysate Fraction Whole Cell Lysate
Lysate Type Cell Lysate
Hintergrund Full Gene Name:
Synonyms:
Applikations-hinweise Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Kommentare

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
Handhabung Aliquot to avoid repeated freezing and thawing.
Lagerung -80 °C
Informationen zur Lagerung Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
Bilder des Herstellers
 image for hES-TW1(human embryonic stem cell) whole cell lysate (non-denatured) (ABIN1339633) hES-TW1(human embryonic stem cell) whole cell lysate (non-denatured)
Produkt verwendet in: Ram, Goren, Amit, Shoresh, Yosef, Ernst, Kellis, Gymrek, Issner, Coyne, Durham, Zhang, Donaghey, Epstein, Regev, Bernstein: "Combinatorial patterning of chromatin regulators uncovered by genome-wide location analysis in human cells." in: Cell, Vol. 147, Issue 7, pp. 1628-39, 2011 (PubMed).