Rattete brain tissue lysate (non-denatured)
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- Proteinspezies
- Ratte
- Lysatspezies
- Ratte
- Applikation
- Immunoprecipitation (IP), Western Blotting (WB)
- Verwendungszweck
- Whole tissue lysate
- Spezifität
- Non-denatured
- Produktmerkmale
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Rat brain tissue lysate (non-denatured)
Used Tissue: Brain - Lysate Type
- Tissue Lysate
- Lysate Fraction
- Whole Tissue Lysate
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- Applikationshinweise
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Kommentare
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Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handhabung
- Aliquot to avoid repeated freezing and thawing.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
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Improved coomassie blue dye-based fast staining protocol for proteins separated by SDS-PAGE." in: PLoS ONE, Vol. 8, Issue 11, pp. e81696, (2013) (PubMed).
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Improved coomassie blue dye-based fast staining protocol for proteins separated by SDS-PAGE." in: PLoS ONE, Vol. 8, Issue 11, pp. e81696, (2013) (PubMed).
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- Hintergrund
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