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Rattete brain tissue lysate (non-denatured)

IP, WB Whole Tissue Lysate Tissue Lysate Reaktivität: Ratte
Produktnummer ABIN1339624
  • Proteinspezies
    Ratte
    Lysatspezies
    Ratte
    Applikation
    Immunoprecipitation (IP), Western Blotting (WB)
    Verwendungszweck
    Whole tissue lysate
    Spezifität
    Non-denatured
    Produktmerkmale
    Rat brain tissue lysate (non-denatured)
    Used Tissue: Brain
    Lysate Type
    Tissue Lysate
    Lysate Fraction
    Whole Tissue Lysate
  • Applikationshinweise
    Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
    Kommentare

    Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
    Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
    Handhabung
    Aliquot to avoid repeated freezing and thawing.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
  • Májek, Riedelová-Reicheltová, Pecánková, Dyr: "Improved coomassie blue dye-based fast staining protocol for proteins separated by SDS-PAGE." in: PLoS ONE, Vol. 8, Issue 11, pp. e81696, (2013) (PubMed).

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