anti-Human Kallikrein 13 Antikörper für Immunoprecipitation

Recommended Kallikrein 13 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Kallikrein-Related Peptidase 13 (KLK13) Antikörper
  • KLK-L4
  • KLKL4
  • Egfbp-2
  • mGk-13
  • kallikrein related peptidase 13
  • kallikrein related-peptidase 13
  • KLK13
  • Klk13
Epitop
AA 19-262
11
6
2
2
2
2
2
1
1
1
1
1
1
1
Reaktivität
Human
72
21
20
4
3
3
3
1
Wirt
Ziege
49
22
3
Klonalität
Polyklonal
Konjugat
Dieser Kallikrein 13 Antikörper ist unkonjugiert
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunoprecipitation (IP), Neutralization (Neut), ELISA, Western Blotting (WB)
53
19
13
11
8
3
2
2
1
1
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Produktnummer ABIN292875
$ 1,123.83
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN2294898 IP Neut ELISA WB Goat IgG AA 19-262 Anmelden zum Anzeigen Polyclonal 0

General

Antigen Kallikrein-Related Peptidase 13 (KLK13) Antikörper
Epitop AA 19-262
(11), (6), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(72), (21), (20), (4), (3), (3), (3), (1)
Wirt Ziege
(49), (22), (3)
Klonalität
Polyklonal
Konjugat Dieser Kallikrein 13 Antikörper ist unkonjugiert
(4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunoprecipitation (IP), Neutralization (Neut), ELISA, Western Blotting (WB)
(53), (19), (13), (11), (8), (3), (2), (2), (1), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Kallikrein 13 Antikörper

Target Details Kallikrein 13 Anwendungsinformationen Handhabung Bilder
Spezifität Selected for its ability to recognize human KLK13 in direct ELISAs and Western Blots. In these formats, this antibody shows ~10 % cross-reactivity with rhKallikrein 8, and less than 5 % cross-reactivity with rhKallikrein 11, rhKallikrein 14, and rhKallikrein 15.
Reinigung Affinity purified
Immunogen NS0-derived rhKLK13 (aa19-262).
Isotyp IgG

Target Details Kallikrein 13

Produktdetails anti-Kallikrein 13 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung KLK13 / Kallikrein 13 (KLK13 Antibody Abstract)
Hintergrund Name/Gene ID: KLK13
Subfamily: Serine S1
Family: Protease

Synonyms: KLK13, Kallikrein 13, Kallikrein-13, KLK-L4, KLKL4, Kallikrein-like protein 4, Kallikrein-like gene 4
Gen-ID 26085
UniProt Q9UKR3
Pathways Komplementsystem

Anwendungsinformationen

Produktdetails anti-Kallikrein 13 Antikörper Target Details Kallikrein 13 Handhabung Bilder zurück nach oben
Applikationshinweise Approved: ELISA, IP, Neut, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human KLK13. The detection limit for rhKLK13 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rhKLK13 from conditioned media of transfected NS0 cells. The recovered rhKLK13 can be detected by western blot analysis using a monoclonal antibody. Neutralization: This antibody was used to inhibit/neutralize the enzymatic activity of rhKLK13. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate, Boc-VPR-AMC. 50 % of the proteolytic activity was inhibited by the antibody at approximately 8.6 μg/mL (IC50) under conditions in which the enzyme was present at 1 μg/mL and the substrate concentration was 100 M. Considering the molecular masses of the enzyme (28 kD) and the antibody (150 kD), IC50 was achieved at approximately 2:1 Molar ratio of the antibody to the enzyme. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human KLK13. The detection limit for rhKLK13 is approximately 1.0 ng/well.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Kallikrein 13 Antikörper Target Details Kallikrein 13 Anwendungsinformationen Bilder zurück nach oben
Format Lyophilized
Rekonstitution deionized water
Konzentration Lot specific
Buffer Lyophilized.