anti-Human C9 Antikörper für Western Blotting

Recommended C9 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Complement Component C9 (C9) Antikörper
  • LOC100037951
  • LOC100136130
  • c9
  • wu:fb60b05
  • wu:fd50e04
  • zgc:112272
  • complement C9
  • complement component 9
  • complement component 9 L homeolog
  • C9
  • c9
  • c9.L
Epitop
AA 191-220, Center
15
9
8
4
3
2
2
2
2
1
1
Reaktivität
Human
117
18
14
2
Wirt
Kaninchen
65
46
7
2
1
1
Klonalität
Polyklonal
Konjugat
Dieser C9 Antikörper ist unkonjugiert
6
6
6
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
85
63
46
23
16
13
12
7
6
2
2
2
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN657704
$ 335.50
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
3.0797327 ABIN4299959 ICC IF WB Rabbit IgG Center Anmelden zum Anzeigen Polyclonal 1
3.0797327 ABIN1534796 ELISA WB Rabbit IgG AA 181-230 Anmelden zum Anzeigen Polyclonal 0
3.0797327 ABIN3180514 ELISA WB Rabbit IgG Internal Region Anmelden zum Anzeigen Polyclonal 0
3.0797327 ABIN1866979 ICC IHC WB Rabbit IgG AA 22-265 Anmelden zum Anzeigen Polyclonal 0
3.0797327 ABIN5530731 IHC (p) WB Rabbit Ig Fraction AA 191-220 Anmelden zum Anzeigen Polyclonal 0
3.0797327 ABIN5692808 ELISA WB Rabbit AA 289-515 Anmelden zum Anzeigen Polyclonal 0
3.0797327 ABIN1876823 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
3.0797327 ABIN5609870 ELISA WB Goat Internal Region Anmelden zum Anzeigen Polyclonal 0
3.0797327 ABIN2896757 IF/ICC IHC IP WB Rabbit AA 22-265 Anmelden zum Anzeigen Polyclonal 0
3.0797327 ABIN5774093 IHC (p) WB Mouse IgG1 kappa Anmelden zum Anzeigen 64E9 0
3.0797327 ABIN5573491 WB Rabbit Anmelden zum Anzeigen Polyclonal 0
3.0797327 ABIN4299958 IHC IHC (p) WB Rabbit IgG Center Anmelden zum Anzeigen Polyclonal 0
3.0797327 ABIN3048399 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
3.0797327 ABIN5859483 ELISA WB Goat AA 205-216 Anmelden zum Anzeigen Polyclonal 0
3.0797327 ABIN2997536 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
3.0797327 ABIN1851158 ELISA WB Rabbit Internal Region Anmelden zum Anzeigen Polyclonal 0
1 ABIN316351 ELISA IF IHC WB Mouse IgG1 kappa Anmelden zum Anzeigen C9 53 0
1 ABIN4994039 ELISA WB Mouse IgG1 kappa Anmelden zum Anzeigen 22 0
1 ABIN4299956 FACS IA IHC IHC (fro) IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen X197 1
1 ABIN1714714 IF (p) IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0

General

Antigen Complement Component C9 (C9) Antikörper
Epitop AA 191-220, Center
(15), (9), (8), (4), (3), (2), (2), (2), (2), (1), (1)
Reaktivität Human
(117), (18), (14), (2)
Wirt Kaninchen
(65), (46), (7), (2), (1), (1)
Klonalität (Klon)
Polyklonal   ( )
Konjugat Dieser C9 Antikörper ist unkonjugiert
(6), (6), (6), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(85), (63), (46), (23), (16), (13), (12), (7), (6), (2), (2), (2), (1), (1)
Pubmed 3 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-C9 Antikörper

Target Details C9 Anwendungsinformationen Handhabung Referenzen für anti-C9 Antikörper (ABIN657704) Bilder
Spezifität This C9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 190-220 amino acids from the Central region of human C9.
Reinigung This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This C9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-220 AA from the Central region of human C9.
Klon RB33918
Isotyp Ig Fraction
Plasmids, Primers & others

Target Details C9

Produktdetails anti-C9 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-C9 Antikörper (ABIN657704) Bilder zurück nach oben
Antigen
Andere Bezeichnung C9 (C9 Antibody Abstract)
Hintergrund This gene encodes the final component of the complement system. It participates in the formation of the Membrane Attack Complex (MAC). The MAC assembles on bacterial membranes to form a pore, permitting disruption of bacterial membrane organization. Mutations in this gene cause component C9 deficiency. [provided by RefSeq].
Synonyms: Complement component C9,C9,
Molekulargewicht 63173 DA
Gen-ID 735
NCBI Accession NP_001728
UniProt P02748
Pathways Komplementsystem

Anwendungsinformationen

Produktdetails anti-C9 Antikörper Target Details C9 Handhabung Referenzen für anti-C9 Antikörper (ABIN657704) Bilder zurück nach oben
Applikations-hinweise WB = 1:1000, IHC (p) = 1:10-50
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-C9 Antikörper Target Details C9 Anwendungsinformationen Referenzen für anti-C9 Antikörper (ABIN657704) Bilder zurück nach oben
Format Liquid
Konzentration 0.36 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung C9 Antibody (Center) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Haltbarkeit 6 months

Referenzen für anti-C9 Antikörper (ABIN657704)

Produktdetails anti-C9 Antikörper Target Details C9 Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Rajaraman, Brenner, Neta, Pfeiffer, Wang, Yeager, Thomas, Fine, Linet, Rothman, Chanock, Inskip: "Risk of meningioma and common variation in genes related to innate immunity." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 19, Issue 5, pp. 1356-61, 2010

Bunkenborg, Pilch, Podtelejnikov, Wi?niewski: "Screening for N-glycosylated proteins by liquid chromatography mass spectrometry." in: Proteomics, Vol. 4, Issue 2, pp. 454-65, 2004

Hofsteenge, Blommers, Hess, Furmanek, Miroshnichenko: "The four terminal components of the complement system are C-mannosylated on multiple tryptophan residues." in: The Journal of biological chemistry, Vol. 274, Issue 46, pp. 32786-94, 2000

Bilder

Produktdetails anti-C9 Antikörper Target Details C9 Anwendungsinformationen Handhabung Referenzen für anti-C9 Antikörper (ABIN657704) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Complement Component C9 (C9) (AA 191-220), (Center) antibody (ABIN657704) C9 Antibody (Center) (ABIN657704) western blot analysis in ZR-75-1 cell line lysates ...
Western Blotting (WB) image for anti-Complement Component C9 (C9) (AA 191-220), (Center) antibody (ABIN657704) Anti-C9 Antibody (Center) at 1:1000 dilution + human liver lysate Lysates/proteins at...
'Independent Validation' Siegel
Antigen C9
Validierte Anwendung Western Blotting
Positivkontrolle Human endothelial cells with nanoparticle treatment
Negativkontrolle Human endothelial cells and astrocytes without nanoparticle treatment
Bewertung

Passed. ABIN657704 specifically recognizes C9 in human endothelial cells.

Primärantikörper ABIN657704
Sekundärantikörper anti-rabbit IgG HRP-linked antibody (Cell Signaling, 7074S)
Protokoll
  • Grow human endothelial cells and astrocytes in EndoGRO-MV Complete Media kit (Millipore, SCME004, lot 2885245) supplemented with serum (Millipore, lot 1000958) at 37°C and 5% CO2 in 8ml on a petri dish to 80-90 % confluency.
  • Add PVP coated silver nanoparticles (50nm Silver Nanospheres, Nanocomposix USA lot no JRC0234) to the endothelial cells for 24h and 48h.
  • Lyse 106 cells in 100µl per well cold lysis buffer (6M Urea, 2M Thiourea, 10mM DTT).
  • Determine total protein content of the lysates using Qubit Protein Assay Kit (ThermoFisher Scientific, Q33211).
  • Denature 20µg of total protein for 5min at 95°C in 5µl Pierce LDS Sample Buffer, Non-Reducing (Thermo Scientific, b31010, lot 151109002) and separate proteins in an XCell SureLock Mini-Cell (Life Technologies, EI0001, lot 007469934) in a Bolt 4-12% Bis-Tris Plus gel (Thermo scientific, NW04125BOX, lot 17120471) for 60min at 100V.
  • Transfer proteins onto PVDF membrane (Sigma-Aldrich, IPFL00005) with a Western blotting system for 20min at 18V.
  • Block the membrane with TBST and 5% non-fat milk powder (Sigma-Aldrich, 70166-500G, lot SZBF3420V) for 1h at RT.
  • Incubation with primary rabbit anti-C9 antibody (antibodies-online, ABIN657704, lot SA110711AL) diluted 1:1000 TBST and 5% non-fat milk powder for ON at 4°C.
  • Wash membrane 3x for 15min with TBST.
  • Incubation with secondary anti-rabbit IgG HRP-linked antibody (Cell Signaling, 7074S) diluted 1:10000 in TBST and 5% non-fat milk powder for 2h at RT.
  • Wash membrane 3x for 15min with TBS.
  • Reveal protein bands using Luminata Forte Western HRP Substrate (Millipore, WBLUF0100) on a UVP multispectral imaging system (AH diagnostics, Denmark).
  • Incubate the membrane in Restore Plus Western blot Stripping Buffer (Thermo Scientific, 46430, lot RL241559) for 20min at RT.
  • Wash membrane 3x for 15min with TBST.
  • Incubation with rabbit anti-GAPDH antibody (Cell Signaling Technology, 2118) diluted 1:5000 in TBST and 5% non-fat milk powder for 2h at RT.
  • Wash membrane 3x for 15min with TBS.
  • Reveal protein bands as described above.
Anmerkungen

Upon addition of the nanoparticles, the endothelial cells’ proteome revealed high expression of C9 at the 24h time point and reduced expression at the 48h time point. We validated the proteomics results by western blotting and we confirm that the C9 antibody ABIN657704 reveals a protein of the expected molecular weight (60kDa) in lysates of endothelial cells. The protein band is only visible in the nanoparticle treated cells but not the negative controls.

Validierungsbilder
Western Blotting validation image for anti-Complement Component C9 (C9) (AA 191-220), (Center) antibody (ABIN657704)

Immunoblot using ABIN657704 on human endothelial cell and human astrocytes lysates...