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YWHAE ELISA Kit

YWHAE Reaktivität: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN6976228
  • Target Alle YWHAE ELISA Kits anzeigen
    YWHAE (14-3-3 epsilon (YWHAE))
    Reaktivität
    • 3
    • 1
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    • 1
    • 1
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    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    78 pg/mL - 5000 pg/mL
    Untere Nachweisgrenze
    78 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    For the quantitative determination of human 14-3-3 protein epsilon (YWHAE) concentrations in serum, plasma, tissue homogenates, cell lysates.
    Proben
    Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of human YWHAE. No significant cross-reactivity or interference between human YWHAE and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human YWHAE and all the analogues, therefore, cross reaction may still exist.
    Sensitivität
    19.5 pg/mL
    Bestandteile
    • Assay plate
    • Standard
    • HRP-avidin (100 x concentrate)
    • Biotin-antibody (100 x concentrate)
    • Sample Diluent
    • HRP-avidin Diluent
    • Biotin-antibody Diluent
    • Wash Buffer (25 x concentrate)
    • TMB Substrate
    • Stop Solution
    • Adhesive Strip
    Top Product
    Discover our top product YWHAE ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Probenmenge
    100 μL
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare reagents, samples and standards as instructed.
    2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C.
    3. Remove the liquid of each well, don't wash.
    4. Add 100 µL Biotin-antibody (1x) to each well. Incubate 1 hour at 37 °C.
    5. Aspirate and wash 3 times.
    6. Add 100 µL HRP-avidin (1x) to each well. Incubate 1 hour at 37 °C
    7. Aspirate and wash 5 times.
    8. Add 90 μL of TMB Substrate to each well. Incubate for 15-30 minutes at 37 °C. Protect from light.
    9. Add 50 µL Stop Solution to each well. Read at 450 nm within 5 minutes.
    Aufbereitung der Reagenzien
    1. Biotin-antibody (1x) - Centrifuge the vial before opening. Biotin-antibody requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of Biotin-antibody + 990 μL of Biotin-antibody Diluent.
    2. HRP-avidin (1x) - Centrifuge the vial before opening. HRP-avidin requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of HRP-avidin + 990 μL of HRP-avidin Diluent.
    3. Wash Buffer (1x) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 mL of Wash Buffer Concentrate (25 x) into deionized or distilled water to prepare 500 mL of Wash Buffer (1 x).
    4. Standard Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 mL of Sample Diluent. Do not substitute other diluents. This reconstitution produces a stock solution of 5000 pg/mL. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. Pipette 250 μL of Sample Diluent into each tube (S0-S6). Use the stock solution to produce a 2-fold dilution series (below). Mix each tube thoroughly before the next transfer. The undiluted Standard serves as the high standard (5000 pg/mL). Sample Diluent serves as the zero standard (0 pg/mL).
    Note:
    • Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit.
    • Bring all reagents to room temperature (18-25 °C) before use for 30 min.
    • Prepare fresh standard for each assay. Use within 4 hours and discard after use.
    • Making serial dilution in the wells directly is not permitted.
    • Please carefully reconstitute Standards according to the instruction, and avoid foaming and mix gently until the crystals have completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10 μL for once pipetting.
    • Distilled water is recommended to be used to make the preparation for reagents. Contaminated water or container for reagent preparation will influence the detection result.
    Aufbereitung der Proben
    • It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
    • If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
    • If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
    • Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).
    Testpräzision
    Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.
    Haltbarkeit
    6 months
  • Target Alle YWHAE ELISA Kits anzeigen
    YWHAE (14-3-3 epsilon (YWHAE))
    Andere Bezeichnung
    tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (YWHAE Produkte)
    Synonyme
    14-3-3 ELISA Kit, 14-3-3 epsilon ELISA Kit, 14-3-3-e ELISA Kit, 14-3-3-epsilon ELISA Kit, 14-3-30epsilon ELISA Kit, 14-3-3EPSILON ELISA Kit, 14-3-3e ELISA Kit, 14-3-3omicron ELISA Kit, 1433epsilon ELISA Kit, 143E_DROME ELISA Kit, CG31196 ELISA Kit, CT24092 ELISA Kit, D14-3-3e ELISA Kit, D14-3-3epsilon ELISA Kit, Dmel\\CG31196 ELISA Kit, EK3-5 ELISA Kit, PAR-5 ELISA Kit, PAR5 ELISA Kit, Par-5 ELISA Kit, SR3-9 ELISA Kit, Su(Raf)3B ELISA Kit, anon-WO0172774.141 ELISA Kit, anon-WO02059370.52 ELISA Kit, d14-3-3epsilon ELISA Kit, eps ELISA Kit, l(3)j2B10 ELISA Kit, par-5 ELISA Kit, 14-3-3epsilon ELISA Kit, kcip-1 ELISA Kit, mdcr ELISA Kit, mds ELISA Kit, LOC581376 ELISA Kit, Ywhae ELISA Kit, ACYPI002517 ELISA Kit, YWHAE ELISA Kit, 14-3-3E ELISA Kit, KCIP-1 ELISA Kit, MDCR ELISA Kit, MDS ELISA Kit, AU019196 ELISA Kit, fe38c12 ELISA Kit, wu:fb48f06 ELISA Kit, wu:fe38c12 ELISA Kit, wu:fi22e10 ELISA Kit, ywhae ELISA Kit, zgc:55377 ELISA Kit, zgc:77062 ELISA Kit, CG31196 gene product from transcript CG31196-RD ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon ELISA Kit, 14-3-3 epsilon ELISA Kit, 14-3-3 epsilon protein ELISA Kit, 14-3-3 protein epsilon ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon S homeolog ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide 1 ELISA Kit, 14-3-3epsilon ELISA Kit, ywhae ELISA Kit, LOC581376 ELISA Kit, Ywhae ELISA Kit, HCAG_04173 ELISA Kit, PTRG_02836 ELISA Kit, PTRG_04459 ELISA Kit, UREG_07864 ELISA Kit, YWHAE ELISA Kit, ywhae.S ELISA Kit, ywhae1 ELISA Kit
    Hintergrund

    Abbreviation: YWHAE

    Alias: 14-3-3E, FLJ45465, KCIP-1, MDCR, MDS, 14-3-3 epsilon|mitochondrial import stimulation factor L subunit|protein kinase C inhibitor protein-1|tyrosine 3/tryptophan 5 -monooxygenase activation protein,

    UniProt
    P62258
    Pathways
    Neurotrophin Signalübertragung, Myometrial Relaxation and Contraction, M Phase
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