YWHAB ELISA Kit

Produktnummer ABIN6951750

Produktdetails YWHAB ELISA Kit

Target: YWHAB (14-3-3 alpha + beta (YWHAB))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human YWHAB ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu YWHAB

Target: YWHAB (14-3-3 alpha + beta (YWHAB))

Andere Bezeichnung: YWHAB (YWHAB Produkte)

Synonyme: GW128 ELISA Kit, HS1 ELISA Kit, KCIP-1 ELISA Kit, YWHAA ELISA Kit, 1300003C17Rik ELISA Kit, wu:fb22g04 ELISA Kit, wu:fr86a12 ELISA Kit, ywhab ELISA Kit, ywhab2 ELISA Kit, zgc:64092 ELISA Kit, 14-3-3beta ELISA Kit, fb80c08 ELISA Kit, fk41f08 ELISA Kit, wu:fb80c08 ELISA Kit, wu:fd11d01 ELISA Kit, wu:fk41f08 ELISA Kit, ywhab1 ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide b ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta S homeolog ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta L homeolog ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide a ELISA Kit, YWHAB ELISA Kit, Ywhab ELISA Kit, ywhabb ELISA Kit, ywhab.S ELISA Kit, ywhab.L ELISA Kit, ywhaba ELISA Kit

Gen-ID: 7529

UniProt: P31946

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Myometrial Relaxation and Contraction, Maintenance of Protein Location