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PPM1G ELISA Kit

PPM1G Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951685
  • Target Alle PPM1G Produkte
    PPM1G (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human PPM1G ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle PPM1G Produkte
    PPM1G (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G))
    Andere Bezeichnung
    PPM1G (PPM1G Produkte)
    Synonyme
    AI385652 ELISA Kit, Fin13 ELISA Kit, PP2CG ELISA Kit, PP2CGAMMA ELISA Kit, PPP2CG ELISA Kit, protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform ELISA Kit, protein phosphatase, Mg2+/Mn2+ dependent 1G ELISA Kit, protein phosphatase, Mg2+/Mn2+ dependent, 1G ELISA Kit, Ppm1g ELISA Kit, PPM1G ELISA Kit
    Gen-ID
    5496
    UniProt
    O15355
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