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ROBO1 ELISA Kit

ROBO1 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6730772
  • Target Alle ROBO1 ELISA Kits anzeigen
    ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))
    Reaktivität
    • 2
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human ROBO1 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Human ROBO1.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product ROBO1 ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle ROBO1 ELISA Kits anzeigen
    ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))
    Andere Bezeichnung
    ROBO1 (ROBO1 Produkte)
    Synonyme
    DUTT1 ELISA Kit, SAX3 ELISA Kit, AW494633 ELISA Kit, AW742721 ELISA Kit, Gm310 ELISA Kit, ROBO1 ELISA Kit, dutt1 ELISA Kit, roundabout ELISA Kit, sax3 ELISA Kit, MGC122264 ELISA Kit, roundabout-1 ELISA Kit, roundabout guidance receptor 1 ELISA Kit, roundabout, axon guidance receptor, homolog 1 (Drosophila) ELISA Kit, roundabout guidance receptor 1 L homeolog ELISA Kit, ROBO1 ELISA Kit, Robo1 ELISA Kit, robo1 ELISA Kit, robo1.L ELISA Kit
    UniProt
    Q9Y6N7
    Pathways
    Positive Regulation of Endopeptidase Activity
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