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BMPR2 ELISA Kit
BMPR2
Reaktivität: Maus
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6730584
Produktdetails BMPR2 ELISA Kit
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Target
Alle BMPR2 ELISA Kits anzeigen
BMPR2
(Bone Morphogenetic Protein Receptor, Type II (serine/threonine Kinase) (BMPR2))
Reaktivität
Alle Reaktivitäten für BMPR2 ELISA Kits
Maus
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Mouse BMPR-II ELISA Kit.
Proben
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Mouse BMPR-II.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Optimal working dilution should be determined by the investigator.
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu BMPR2
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Target
Alle BMPR2 ELISA Kits anzeigen
BMPR2
(Bone Morphogenetic Protein Receptor, Type II (serine/threonine Kinase) (BMPR2))
Andere Bezeichnung
BMPR-II (BMPR2 Produkte )
Synonyme
BMPR-II ELISA Kit, BMPR3 ELISA Kit, BMR2 ELISA Kit, BRK-3 ELISA Kit, PPH1 ELISA Kit, T-ALK ELISA Kit, 2610024H22Rik ELISA Kit, AL117858 ELISA Kit, AW546137 ELISA Kit, BB189135 ELISA Kit, BMP-2 ELISA Kit, BMPR-2 ELISA Kit, BMPRII ELISA Kit, Gm20272 ELISA Kit, Bmpr-II ELISA Kit, bmprii ELISA Kit, bone morphogenetic protein receptor type 2 ELISA Kit, bone morphogenetic protein receptor, type II b (serine/threonine kinase) ELISA Kit, bone morphogenetic protein receptor, type II (serine/threonine kinase) ELISA Kit, BMPR2 ELISA Kit, bmpr2b ELISA Kit, Bmpr2 ELISA Kit
UniProt
O35607
Pathways
Growth Factor Binding
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