YWHAE ELISA Kit

Produktnummer ABIN6730561

Produktdetails YWHAE ELISA Kit

Target: YWHAE (14-3-3 epsilon (YWHAE))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.34 ng/mL - 80 ng/mL

Untere Nachweisgrenze: 0.34 ng/mL

Applikation: ELISA

Verwendungszweck: Human YWHAE ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human YWHAE.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Reagenzien:

Recommended Dilution for serum and plasma samples2 fold

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu YWHAE

Target: YWHAE (14-3-3 epsilon (YWHAE))

Andere Bezeichnung: YWHAE (YWHAE Produkte)

Synonyme: 14-3-3 ELISA Kit, 14-3-3 epsilon ELISA Kit, 14-3-3-e ELISA Kit, 14-3-3-epsilon ELISA Kit, 14-3-30epsilon ELISA Kit, 14-3-3EPSILON ELISA Kit, 14-3-3e ELISA Kit, 14-3-3omicron ELISA Kit, 1433epsilon ELISA Kit, 143E_DROME ELISA Kit, CG31196 ELISA Kit, CT24092 ELISA Kit, D14-3-3e ELISA Kit, D14-3-3epsilon ELISA Kit, Dmel\\CG31196 ELISA Kit, EK3-5 ELISA Kit, PAR-5 ELISA Kit, PAR5 ELISA Kit, Par-5 ELISA Kit, SR3-9 ELISA Kit, Su(Raf)3B ELISA Kit, anon-WO0172774.141 ELISA Kit, anon-WO02059370.52 ELISA Kit, d14-3-3epsilon ELISA Kit, eps ELISA Kit, l(3)j2B10 ELISA Kit, par-5 ELISA Kit, 14-3-3epsilon ELISA Kit, kcip-1 ELISA Kit, mdcr ELISA Kit, mds ELISA Kit, LOC581376 ELISA Kit, Ywhae ELISA Kit, ACYPI002517 ELISA Kit, YWHAE ELISA Kit, 14-3-3E ELISA Kit, KCIP-1 ELISA Kit, MDCR ELISA Kit, MDS ELISA Kit, AU019196 ELISA Kit, fe38c12 ELISA Kit, wu:fb48f06 ELISA Kit, wu:fe38c12 ELISA Kit, wu:fi22e10 ELISA Kit, ywhae ELISA Kit, zgc:55377 ELISA Kit, zgc:77062 ELISA Kit, CG31196 gene product from transcript CG31196-RD ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon ELISA Kit, 14-3-3 epsilon ELISA Kit, 14-3-3 epsilon protein ELISA Kit, 14-3-3 protein epsilon ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon S homeolog ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide 1 ELISA Kit, 14-3-3epsilon ELISA Kit, ywhae ELISA Kit, LOC581376 ELISA Kit, Ywhae ELISA Kit, HCAG_04173 ELISA Kit, PTRG_02836 ELISA Kit, PTRG_04459 ELISA Kit, UREG_07864 ELISA Kit, YWHAE ELISA Kit, ywhae.S ELISA Kit, ywhae1 ELISA Kit

UniProt: P62258

Pathways: Neurotrophin Signalübertragung, Myometrial Relaxation and Contraction, M Phase