HVEM ELISA Kit

Produktnummer ABIN6730493

Produktdetails HVEM ELISA Kit

Target: HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse HVEM/TNFRSF14 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse HVEM.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu HVEM

Target: HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))

Andere Bezeichnung: HVEM (TNFRSF14 (TNFRSF14 Produkte)

Synonyme: ATAR ELISA Kit, CD270 ELISA Kit, HVEA ELISA Kit, HVEM ELISA Kit, LIGHTR ELISA Kit, TR2 ELISA Kit, Atar ELISA Kit, HveA ELISA Kit, Hvem ELISA Kit, Tnfrs14 ELISA Kit, TNF receptor superfamily member 14 ELISA Kit, tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) ELISA Kit, Tnfrsf14 ELISA Kit, TNFRSF14 ELISA Kit

Substanzklasse: Viral Protein

UniProt: Q80WM9

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints