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CSNK2A1/CK II alpha ELISA Kit

CSNK2A1 Reaktivität: Maus Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6730466
  • Target Alle CSNK2A1/CK II alpha (CSNK2A1) ELISA Kits anzeigen
    CSNK2A1/CK II alpha (CSNK2A1) (Casein Kinase 2 alpha 1 (CSNK2A1))
    Reaktivität
    • 3
    • 3
    • 2
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Mouse CSNK2A1 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Mouse CSNK2A1.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle CSNK2A1/CK II alpha (CSNK2A1) ELISA Kits anzeigen
    CSNK2A1/CK II alpha (CSNK2A1) (Casein Kinase 2 alpha 1 (CSNK2A1))
    Andere Bezeichnung
    CSNK2A1 (CSNK2A1 Produkte)
    Synonyme
    CK2A2 ELISA Kit, CSNK2A1 ELISA Kit, CK2A1 ELISA Kit, CKII ELISA Kit, CSNK2A3 ELISA Kit, CK2A ELISA Kit, Ck2a ELISA Kit, BmCK2a ELISA Kit, wu:fi38e04 ELISA Kit, wu:fi38h03 ELISA Kit, csnk2a1 ELISA Kit, CK-II ELISA Kit, CK2 ELISA Kit, Ckiialpha ELISA Kit, Csnk2a1-rs4 ELISA Kit, ck2a1 ELISA Kit, casein kinase 2 alpha 2 ELISA Kit, casein kinase 2 alpha 1 ELISA Kit, casein kinase 2 alpha subunit ELISA Kit, casein kinase 2, alpha 1 polypeptide ELISA Kit, CK2 protein kinase alpha 2 ELISA Kit, casein kinase II alpha subunit ELISA Kit, Casein kinase II alpha subunit ELISA Kit, casein kinase 2, alpha 1 polypeptide S homeolog ELISA Kit, CSNK2A2 ELISA Kit, CSNK2A1 ELISA Kit, ck2a ELISA Kit, Ck2a ELISA Kit, csnk2a1 ELISA Kit, cka2 ELISA Kit, Ckiialpha ELISA Kit, CK2A1 ELISA Kit, Csnk2a1 ELISA Kit, csnk2a1.S ELISA Kit
    UniProt
    Q60737
    Pathways
    SARS-CoV-2 Protein Interaktom
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